Perbedaan Hasil Pemeriksaan Hematologi yang Segera Dilakukan Pemeriksaan dan yang Ditunda pada Suhu Ruangan dan Refrigerator menggunakan Sysmex XP-300

The Difference Between the Hematology Examination that was Immediately Carried Out and which was Delayed at Room Temperature and Recommended using a Sysmex XP-300

Authors

  • Nathalya Dwi Kartika Sari Universitas Nahdlatul Ulama Surabaya
  • Gilang Nugraha Universitas Nahdlatul Ulama Surabaya https://orcid.org/0000-0002-6309-3430
  • Brina Thursina Dibiasi Universitas Nahdlatul Ulama Surabaya
  • Mirshall Abiedama Prayoga Universitas Nahdlatul Ulama Surabaya

DOI:

https://doi.org/10.33084/bjmlt.v4i2.2962

Keywords:

Hematology, Time, Temperature, Erythrocyte morphology

Abstract

An automatic hematology analyzer (hematology analyzer, ha) is a routine hematology examination tool used extensively in clinical laboratory services. Sysmex XP-300 is the HA 3-part diff that is the most straightforward ha impedance examination of the number of cells based solely on cell sizes. The storage temperature and incubation times affected the complete blood count examination. This study determined the difference between hematology and EDTA's immediate examination of blood samples and was delayed at room temperature and temperature using a Sysmex XP-300. Hopefully, this research could be developed with a method of validation of checks to minimize errors in clinical laboratories. Experimental research methods with a pre-experimental design through the one group afflicted posttest design with a sample of as many as 15 laboratories, and we analyzed with ANOVA. The results of this study have WBC, RBC, HGB, HCT, MCV, MCH, and MCHC different results at room temperatures 2, 24, 48, and 72 hours compared to temperature fluctuations; the PLT parameters alone show no significant difference when it comes to prompt examinations with delays in the application. Morphology of erythrocyte is influenced by temperature, and storage should be hypotensive.

Downloads

Download data is not yet available.

References

A.M Diks,et al.(2019) ‘Impact of blood storage and sample handling on quality of high dimensional flowcytometric data in multicenter clinical research’.Journal of Immunological Methods 475; 112616. https://doi.org/101016/j.jim.2019.06.007
Antwi-Baffour S, Quao E, Kyeremeh R(2013). Prolong store of blood in EDTA has an the effect on the morphology and osmotic fragility of erythrocyte. International Journal of Biomedical Science and Engineering; 1(2):20-23. https://doi.org/10.11648/j.ijbse.20130102.11
Briggs, C., Culp, N., Davis, B., d’Onofrio, G., Zini, G., & Machin, S. J. (2014). ICSH guidelines for the evaluation of blood cell analysers including those used for differential leucocyte and reticulocyte counting. International Journal of Laboratory Hematology, 36(6), 613–627. https://doi.org/10.1111/ijlh.12201
Daves, M., Zagler, E. M., Cemin, R., Gnech, F., Joos, A., Platzgummer, S., & Lippi, G. (2015). Sample stability for complete blood cell count using the Sysmex XN hematological analyzer. Blood Transfusion, 13(4), 576–582. https://doi.org/10.2450/2015.0007-15
Gian Luca Salvagno, Davide Demonte, Francesco Dima, Chiara Bovo, Giuseppe Lippi (2020)’Stability of refrigerated whole blood samples for osmotic fragility test’, Hematol Transfus Cell Ther;42(2); p 134-138. https://doi.org/10/1016/j/htct.2019.06.001
Gunawardena et al.(2017) ‘Reliability of Parameters of Complete Blood Count With Different Storage Conditions’, Journal of Clinical Laboratory Analysis 31:pp.1-6. Doi 10.1002/jcla.22042.
Jain, A. et al. (2018) ‘Storage stability of commonly used haematological parameters at 33°c’, Biochemia Medica. Biochemia Medica, Editorial Office, 28(2 Special Issue). doi: 10.11613/BM.2018.020901.
Keohane, E. M., Smith, L. J. and Walenga, J. M. (2016) Rodaks’s Hematology: Clinical Principles and Application. 5th edn. Missouri: Elsevier.
Rahmania, Y. L., Kuntjoro, T. and Suroto, V. (2019) ‘Proving the Accuracy and Legal Liability of Clinical Laboratory Examination Results Using Automatic Tools’, Jurnal Hukum Kesehatan, 5(2), pp. 358–376.
Rahmanitarini, A., Hernaningsih, Y. and Indrasari, Y. N. (2019) ‘The stability of sample storage for complete blood count (CBC) toward the blood cell morphology’, Bali Medical Journal, 8(2), p. 482. doi: 10.15562/bmj.v8i2.1369.
Ramya D, Vijayambika J and Eswari V, 2020’Effect of room temperature and refrigerated storage on automated hematological parameters’ Indian Journal of Pathology and Oncology; 7(4) p 625-630
Rodak BF, Fritsma GA, and Keohane FA. Hematology, Clinical Principles and Application 4th ed Elsevier Saunder. 2016: 176-180
Sevda Unalli Ozmen, Yesim Ozarda (2021)’ Stability of hematological analytes during 48 hours storage at thre temperatures using Cell-Dyn Hematology analyser, J Med Biochem 40; p 252-260. DOI : 10.5937/jomb0-27945
Tanaka Y, Gondo K, et al.(2014) Performance evaluation of platelet counting by novel fluorescent dye steining in the XN-series automated hematology analyzers. J Clin Lab Anal ; 28: p 341-8.
Tang, O. et al. (2019) ‘Short-Term Stability of Hematologic Parameters in Frozen Whole Blood’, The journal of applied laboratory medicine. NLM (Medline), 4(3), pp. 410–414. doi: 10.1373/jalm.2018.028357.
Tendulkar, A., Jain, P., Gujral, S., Tambe, M., Kenjale, R., & Ganesh, B. (2015). Stability of Selected Hematological Parameters in Stored Blood Samples. Journal of Cell Science & Therapy, 6(5). https://doi.org/10.4172/2157- 7013.1000220
Vives-Corrons, J. L. et al. (2014) ‘Effect of EDTA-anticoagulated whole blood storage on cell morphology examination: A need for standardization’, International Journal of Laboratory Hematology. Blackwell Publishing Ltd, pp. 222–226. doi: 10.1111/ijlh.12170.
Zini, G. (2014) ‘Stability of complete blood count parameters with storage: Toward defined specifications for different diagnostic applications’, International Journal of Laboratory Hematology. Blackwell Publishing Ltd, pp. 111–113. doi: 10.1111/ijlh.12181.

Downloads

Published

2022-04-30

How to Cite

Sari, N. D. K., Nugraha, G., Dibiasi, B. T., & Prayoga, M. A. (2022). Perbedaan Hasil Pemeriksaan Hematologi yang Segera Dilakukan Pemeriksaan dan yang Ditunda pada Suhu Ruangan dan Refrigerator menggunakan Sysmex XP-300: The Difference Between the Hematology Examination that was Immediately Carried Out and which was Delayed at Room Temperature and Recommended using a Sysmex XP-300. Borneo Journal of Medical Laboratory Technology, 4(2), 279–291. https://doi.org/10.33084/bjmlt.v4i2.2962