Pharmacognostic Study and Antioxidant Activity of Mundar (Garcinia forbesii King.) leaves from Banua Botanical Gardens of South Kalimantan

Keywords: Garcinia forbesii King., Mundar, Leaves, Pharmacognostic study, Antioxidant

Abstract

Mundar (Garcinia forbesii King.) is a plant from South Kalimantan. This plant has chemical contents that have potential as medicine. The purpose of this study is to provide a pharmacognostic picture of a specific, nonspecific and determine the antioxidant activity of G. forbesii leaves. Specific parameters include organoleptic, microscopic testing, thin-layer chromatography profiles, and phytochemical screening. Nonspecific parameters include total ash content, acid insoluble ash content, drying loss, water-soluble extract content, and ethanol-soluble extract content. Antioxidant activity was determined by the DPPH method based on IC50 values. Specific parameter test results are green powder, characteristic odor, and sour taste. Microscopic tests showed stomata, upper epidermis, lower epidermis, cell walls, xylem, phloem, palisade tissue, spongy tissue, and cuticles. Garcinia forbesii leaves contain alkaloids, flavonoids, phenols, tannins, and steroids. The TLC profile showed good separation of polar eluents, and yellow spots appeared after spraying DPPH reagents. The non-specific parameter test is the total ash content of 6.52±0.1%; acid insoluble ash content 1.06±0.08%; drying shrinkage 6.43±0.38%; water-soluble extracts 34.3±0.3%; and ethanol-soluble extracts 23.47±0.35%. Ethanol extract of G. forbesii leaves has antioxidant activity with IC50 of 65.7 ppm. Pharmacognostic study fulfills the requirements, and G. forbesii leaves extract has strong antioxidant activity.

Downloads

Download data is not yet available.

References

Alen, Y., Safitri, N., Dachriyanus, Ali, A.M., Ladjis, N.H., & Sargent, M.V. (2008). Rubraxhantone dari Garcinia forbesii king. dan Bioaktivitasnya. Jurnal Riset Kimia, 1(2), 192-201. doi:10.25077/jrk.v1i2.76
Ali, S., Khan, M.R., Irfanullah, Sajid, M., & Zahra, Z. (2018). Phytochemical investigation and antimicrobial appraisal of Parrotiopsis jacquemontiana (Decne) Rehder. BMC Complementary Medicine and Therapies, 18, 43. doi:10.1186/s12906-018-2114-z
Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., & Lightfoot, D.A. (2017). Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants, 6(4), 42. doi:10.3390/plants6040042
Andarini, F., Yasni, S., & Syamsir, E. (2018). Pengembangan Minuman Fungsional Dari Ekstrak Kulit Mundar. Jurnal Teknologi dan Industri Pangan, 29(1), 49-57. doi:10.6066/jtip.2018.29.1.49
Dewi, A.R. (2018). Penetapan Kadar Fenol Dan Tanin Total Serta Uji Aktivitas Antioksidan Metode Frap Pada Ekstrak Etanol Kulit Buah Mundar (Garcinia forbesii King.). Undergraduate Thesis. Banjarbaru, Indonesia: Universitas Lambung Mangkurat.
Hadadi, Z., Nematzadeh, G.A., & Ghahari, S. (2020). A study on the antioxidant and antimicrobial activities in the chloroformic and methanolic extracts of 6 important medicinal plants collected from North of Iran. BMC Chemistry, 14(1), 33. doi:10.1186/s13065-020-00683-5
Handayani, P.A., Ramadani, N.S., & Kartika, D. (2018). Pemungutan tanin propagul mangrove (rhizopora mucronata) dengan pelarut etanol dan aquades sebagai zat warna alami menggunakan metode microwave assisted extraction. Jurnal Kompetensi Teknik, 10(1), 22-27. doi:10.15294/jkomtek.v10i1.17366
Hardiana, R., Rudiyansyah, & Anita, T. (2012). Aktivitas Antioksidan Senyawa Golongan Fenol Dari Beberapa Jenis Tumbuhan Famili Malvaceae. E-Jurnal Kimia Khatulistiwa, 1(1), 8-13.
Kabera, J.N., Semana, E., Mussa, A.R., & He, X. (2014). Plant Secondary Metabolites: Biosynthesis, Classification, Function and Pharmacological Properties. Journal of Pharmacy and Pharmacology, 2(7), 377-392.
Kedare, S.B. & Singh, R.P. (2011). Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology, 48(4), 412-422. doi:10.1007/s13197-011-0251-1
Larasati, F. (2017). Uji Aktivitas Antibakteri Fraksi Dan Senyawa Aktif Daun Seburo (Garcinia forbesii King.) terhadap Escherichia coli ATCC 25922 dan Staphylococcus aureus ATCC 25923. Undergraduate Thesis. Palembang, Indonesia: Universitas Sriwijaya.
Lim, T.K. (2012). Garcinia forbesii. In: Edible Medicinal and Non-Medicinal Plants. Dordrecht, Netherlands: Springer. doi:10.1007/978-94-007-1764-0_6
McBirney, S.E., Trinh, K., Wong-Beringer, A., & Armani, A.M. (2016). Wavelength-normalized spectroscopic analysis of Staphylococcus aureus and Pseudomonas aeruginosa growth rates. Biomedical Optics Express, 7(10), 4034-4042. doi:10.1364/BOE.7.004034
Ministry of Health of the Republic of Indonesia. (2017). Farmakope Herbal Indonesia Edisi II. Jakarta, Indonesia: Directorate General of Pharmaceutical and Medical Devices, Ministry of Health, Republic of Indonesia.
Ministry of Health of the Republic of Indonesia. (1995). Materia Medika Indonesia Jilid VI. Jakarta, Indonesia: Ministry of Health, Republic of Indonesia.
Muchtadi, T.R. & Ayustaningwarno, F. (2010). Teknologi Proses Pengolahan Pangan. Bandung, Indonesia: Alfabeta.
Muthia, R., Saputri, R., & Verawati, S.A. (2019). Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Mundar (Garcinia forbesii king.) Menggunakan Metode DPPH (2,2-Diphenyl-1-Picrylhydrazil). Jurnal Pharmascience, 6(1), 74-82. doi:10.20527/jps.v6i1.6079
Muthia, R., Saputri, R., & Asfia, N. (2018). Uji Aktivitas Antioksidan Fraksi Etil Asetat Kulit Buah Mundar (Garcinia forbesii King) Menggunakan Metode DPPH (2,2-Difenil-1-pikrilhidrazil). Borneo Journal of Pharmascientech, 2(2), 48-58.
Ratnani, R.D., Hartati, I., Anas, Y., Endah, D.P., & Khilyati, D.D.D. (2017). Standardisasi Spesifik Dan Non Spesifik Ekstraksi Hidrotropi Andrographolid Dari Sambiloto (Andrographis paniculata). In: Prosiding Seminar Nasional Peluang Herbal Sebagai Alternatif Medicine Tahun 2015, 147-155. Semarang, Indonesia: Universitas Wahid Hasyim. doi:10.31942/jiffk.v0i0.1358
Salamah, N. & Widyasari, E. (2015). Aktivitas Antioksidan Ekstrak Metanol Daun Kelengkeng (Euphoria longan (L) Steud.) dengan Metode Penangkapan Radikal 2,2’-Difenil-1-Pikrilhidrazil. Pharmaciana: Jurnal Kefarmasian, 5(1), 25-34. doi:10.12928/pharmaciana.v5i1.2283
Sapna, S., Avinash, K., Mukul, T., & Pathak, A.K. (2008). Pharmacognostic and phytochemical investigation of Stevia rebaudiana. Pharmacognosy Magazine, 4(13), 89-94.
Zhang, Q.W., Lin, L.G., & Ye, W.C. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine, 13, 20. doi:10.1186/s13020-018-0177-x
Published
2020-11-30
How to Cite
Sutomo, S., Kamali, D. N., Arnida, A., Normaidah, N., & Sriyono, A. (2020). Pharmacognostic Study and Antioxidant Activity of Mundar (Garcinia forbesii King.) leaves from Banua Botanical Gardens of South Kalimantan. Borneo Journal of Pharmacy, 3(4), 209-215. https://doi.org/10.33084/bjop.v3i4.1541
Section
Pharmacognosy-Phytochemistry