TY - JOUR AU - Yamin, Yamin AU - Ruslin, Ruslin AU - Sabarudin, Sabarudin AU - Sida, Nurramadhani A AU - Kasmawati, Henny AU - Diman, La Ode Muhammad PY - 2020/11/30 Y2 - 2024/03/29 TI - Determination of Antiradical Activity, Total Phenolic, and Total Flavonoid Contents of Extracts and Fractions of Langsat (Lansium domesticum Coor.) Seeds JF - Borneo Journal of Pharmacy JA - Borneo J Pharm VL - 3 IS - 4 SE - Natural Product Development DO - 10.33084/bjop.v3i4.1500 UR - https://journal.umpr.ac.id/index.php/bjop/article/view/1500 SP - 249-256 AB - <p style="text-align: justify;"><em>Lansium domesticum</em> Coor. is a fruit species from the Meliaceae family, which is a tropical plant native to Southeast Asia. Local citizens call it&nbsp;<em>langsat</em>, <em>longkong</em>, or <em>duku</em> and have used it as traditional medicine. The seeds of <em>L. domesticum </em>are used as a fever medicine, its bark is used to treat scorpion sting, and its leaves are used to repel mosquitoes. Because of its various uses, it is necessary to explore the antiradical potential of <em>L. domesticum </em>seeds. This study aims to determine the antiradical potential of <em>L. domesticum </em>seeds extract and fractions by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and to discover compounds that act as antiradical. <em>Lansium domesticum </em>seed powder was macerated with methanol, and then the extract was concentrated using a rotary evaporator and fractionated by <em>n</em>-hexane and ethyl acetate. The antiradical assay was conducted on extract and fractions by using DPPH&nbsp;<span style="font-size: calc(var(--rem) * 1px * 1.0625); letter-spacing: 0px;">radicals</span><span style="font-size: calc(var(--rem) * 1px * 1.0625); letter-spacing: 0px;">. Phenolic and flavonoid contents from extract and fractions were also tested. The ethyl acetate fraction obtained strong antiradical potential with an IC</span><sub style="letter-spacing: 0px;">50</sub><span style="font-size: calc(var(--rem) * 1px * 1.0625); letter-spacing: 0px;"> value of 8.938 ± 0.031 µg/mL. Total phenolic and flavonoid contents of ethyl acetate fraction were higher with values ​​of 58.25 ± 0.501 mgGAE/g sample and 75.123 ± 0.175 mgQE/g sample, respectively. Correlation of phenolic and flavonoid contents, which inhibited radicals had R</span><sup style="letter-spacing: 0px;">2</sup><span style="font-size: calc(var(--rem) * 1px * 1.0625); letter-spacing: 0px;"> values ​​of 0.9182 and 0.7658. Ethyl acetate fraction of </span><em>L. domesticum </em><span style="font-size: calc(var(--rem) * 1px * 1.0625); letter-spacing: 0px;">seeds had very strong antiradical activity. Further isolation is expected to be conducted to discover which compounds are the most responsible as antiradical.</span></p> ER -