Borneo Journal of Medical Laboratory Technology

Uji Stabilitas Pooled Sera Yang Disimpan Di Suhu 2 - 8ºc Pada Pemeriksaan Albumin

2023-11-11T01:51:12+00:00

The health laboratory is one of the health services for the community in helping to establish a diagnosis, monitoring treatment results, and determining the prognosis of a disease. Control serum is very important for assessing performance in the laboratory, including instrument and reagent quality. Some laboratories use pooled serum as control serum for daily examination. Albumin is the most abundant plasma protein in the human body, which is about 55-60% and the total normal serum protein level is 3.8-5.0%. This study aims to test the stability of pooled sera on examination of albumin stored in the refrigerator at a temperature of 2 - 8ºC for 2 weeks. This research method uses observational research methods with precision data analysis techniques (accuracy). The results of the study of examining albumin levels using the Bromcresol Green (BCG) method obtained serum stability results on day 1 to day 5, where the CV value from the albumin level examination entered the maximum CV value on albumin, which was 6. The CV value for the 5th day covers, day 1 to day 2 = 1.99. Day 1 to day 3 = 2.17. Day 1 to day 4 = 4.27. And day 1 to day 5 = 4.84. On day 6 to day 14 and so on, the serum is found to be unstable and the value does not enter the maximum CV value.

Imunogenesitas Protein Pilli Salmonella Typhi Pada Mencit Balb/C Dengan Mengukur Kadar TNF-α

2023-11-11T01:59:24+00:00

Typhoid fever, often called typhoid disease, is caused by Salmonella enterica subspecies enterica serevoar typhi (Salmonella typhi). The virulence of S.typhi bacteria depends on its ability to invade host cells consisting of several components such as cell wall antigens, flagellum antigens, Vi antigens and pilli antigens. Pilli is one of the virulence factors of S.typhi bacteria, which functions as a tool for adhesion or interaction between bacteria. The study was conducted to determine how the immunogenicity of pilli protein of S.typhi bacteria isolate BA07.4 was immunized in Balb/C mice at varying doses (2-6μg) by measuring TNF- α. The analysis carried out obtained the optimum results of TNF-α levels at a dose of 3μg with an average level of 62.152 pg / ml. In the research conducted, it was found that S.typhi pilli protein can induce specific antibodies against pilli antigens. This shows that the pilli protein is immunogenic, can cause an immune response and can induce antibodies that can recognize the immunized pilli protein sub-unit.

Aktivitas Antibakteri Kombinasi Ekstrak Biji Manjakani (Quercus infectoria) Dan Daun Sirih Merah (Piper crocatum) Terhadap Pertumbuhan Bakteri Klebsiella pneumoniae

2023-11-11T02:07:29+00:00

Potential plants found in Indonesia that can be used for the treatment of urinary tract infections include: Manjakani seeds (Quercus infectoria) and red betel leaves (Piper crocatum). Manjakani seeds are pharmacologically claimed to have biological activities such as anti-inflammatory, antibacterial and antifungal. Red betel leaf has the potential as an antibacterial in the treatment of infectious diseases. The purpose of this study was to determine the antibacterial activity of the combination of manjakani seed extract and red betel leaf in inhibiting the growth of Klebsiella pneumoniae bacteria. The type of research used istrue experiment study with a completely randomized design (CRD) with three factors, namely the type of solvent n-hexane, ethyl acetate, ethanol 96%, ratio (1:1,1:2,2:1) and concentration (75%,100%) with each treatment being repeated twice. This research was conducted in the microbiology laboratory, Labkes, East Kalimantan. The population in this study is the manjakani plant and red betel plant and tested using the agar diffusion technique method Kirby Bauer with positive control (Ciprofloxacin) and negative control (aquadest). Data analysis used is One Way ANOVA and advanced test methods Duncan with a significance level of 0,05. The results obtained showed that there was an effect of antibacterial activity of Manjakani seed extract and red betel leaf on growth K. pneumoniae. The most effective concentration was found in ethanol 96% extract, (2:1 combination of 100% concentration) of 13,97 mm and an effectiveness of 55,74% with a positive control ciprofloxacin 5µg of 25,06 mm. It can be concluded that the combined extract of manjakani seeds and red betel leaves has antibacterial activity against bacteria Klebsiella pneumoniae,but the potential is still low of ciprofloxacin.

Optimasi Konsentrasi Primer Dan Suhu Annealing Dalam Mendeteksi Gen Blaz Pada Bakteri Methicillin Resistant Staphylococcus Aureus Di Udara

2023-11-11T02:16:45+00:00

Staphylococcus aureus is a species of bacteria that is often identified as a source of infection and has increased the number of cases of resistance. Resistance to Penicillin-class antibiotics in S. aureus is associated with plasmids containing the blah-Z gene encoding ß-Lactamase. Some factors that can be modified to obtain optimal test results in PCR are annealing temperature and primary concentration. This study aims to identify the primary concentration and annealing temperature that produce PCR products with the best band in detecting the blaZ gene in Metichilin Resistant Staphylococcus aureus (MRSA) bacteria. The research design used used experimental methods. The results of the study obtained were PCR products at primary concentrations of 10 μM were clearly visible, thick and clean, compared to primary concentrations of 5 μM. Annealing temperature with the best band results at temperatures of 61.8 °C and 58.7°C. This study concluded the primary concentration to obtain a clearly visible band result of 10 μM. The annealing temperature of 61.8°C produced the best band in the sample code R.PG. U4, R.V.U2, and R.151.U2 temperatures. While the sample code R.IGD. U5 band results best at annealing temperature 58.7°C.

Hubungan Jumlah Monosit Dan Nilai Laju Endap Darah (LED) Terhadap Lama Pengobatan Pasien Tuberculosis

2023-11-11T03:35:41+00:00

Tuberculosis (TBC) is a bacterial infection caused by Mycobacterium tuberculosis. The duration of TBC treatment is routinely 6 months; otherwise, resistance will develop. TBC can increase leukocytes, including monocytosis as an inflammatory response and an elevated ESR. The aim of this study was to determine the relationship between the number of monocytes and the ESR value on the duration of tuberculosis treatment in patients at the Respira Lung Hospital, Yogyakarta City. The study was conducted in June and July 2023 at the Respira Pulmonary Hospital in Yogyakarta City. The study sample was 32 tuberculosis patients and blood was drawn to determine the monocyte number and ESR value. The results showed that the average number of monocytes was 7% (normal), the ESR value was 41.59 mm/hour (high) with the average duration of tuberculosis treatment for respondents was 3 months. Spearman test obtained Sig p<0.05. This study concluded that there is a correlation between the number of monocytes and the ESR value on the duration of TB treatment in patients at the Respira Lung Hospital in Yogyakarta City, with the direction of the relationship reversed.

Pemeriksaan Pediculus Humanus Capitis Pada Anak Sekolah Dasar Di Pesantren Hidayatullah Kota Palangka Raya Tahun 2023

2023-11-11T03:46:36+00:00

Head lice (Pediculus humanus capitis) are parasites that live on the hair and scalp of humans by sucking blood from the human scalp. The transmission of head lice can occur directly (hair to hair) and indirectly through shared accessories. A pesantren is one of the places where several people gather in the same location, and this is a primary factor in the spread of Pediculus humanus capitis infection. The research objective is to determine the number of children infected with Pediculus humanus capitis. The research method used is descriptive. The sample in this study consisted of 14 individuals taken using total sampling technique. The research results showed that there were 4 students (29%) infected with Pediculus humanus capitis at Pesantren Hidayatullah in Palangka Raya City, and 10 students (71%) were not infected with Pediculus humanus capitis. The positive results are believed to be due to the habit of using hair accessories such as shared hair combs, which increase the occurrence of Pediculus humanus capitis infection.

Potensi Ikan Seluang (Rasbora spp.) Dan Ikan Lais (Kryptopterus spp.) Dalam Meningkatkan Performa Tikus Wistar (Rattus Norvegicus) Malnutrisi

2023-11-11T04:13:57+00:00

Malnutrition caused by a lack of nutritional intake in children can have a negative impact on physical growth, cognitive function development and immune levels. Malnutrition conditions which are persistently allowed without special intensive and consistent treatment will result in stunting. The efforts that can be made to overcome the incidence of stunting are by fulfilling complex nutrition which can come from various sources, one of which is by consuming seluang fish and lais fish which have high calorie, protein and fat content since they have the potency to improve nutrition in children, especially children who suffer from stunting. The aim of this research was to see the potential of seluang and lais fish in improving performance in Wistar rats induced by malnutrition. This research type is a true experimental research with a pretest-posttest with control group design using 25 male white rats (Rattus norvegicus) Wistar strain aged 2-4 weeks which will be divided into 5 groups, namely K(+), K(-) , P1, P2, P3. The Wilcoxon test was also carried out to analyze the physical endurance of mice and it was found that there was a significant difference in the number of mice falls (p=0.000). The results showed that there was a significant increase physical endurance between before and after the treatment in malnutrition induced rats. The P3 group had the most effective dose in improving rat performance.

Pembuatan Sediaan Gel Arang Tempurung Kelapa Dan Uji Daya Hambat Terhadap Bakteri Klebsiella pneumoniae

2023-11-11T04:29:09+00:00

Klebsiella pneumoniae is a gram-negative rod-shaped bacterium that is a member of the Enterobacteriaceae group of bacteria and is pathogenic and can infect humans. Currently, many K. pneumoniae bacteria are resistant to antibiotics, giving rise to new problems in the health sector that must be addressed immediately by utilizing biodiversity in the form of medicinal plants such as coconut trees, which can produce coconut shells that can be made into charcoal. The results of the phytochemical analysis show that coconut shell charcoal contains many active compounds that have been used in the cosmetic and health industries. The purpose of this study was to obtain an optimal and effective coconut shell charcoal extract gel formula for inhibiting the growth of K. pneumoniae bacteria. This study included a true experimental post-only control group with different gel concentration groups, namely 3%, 6%, and 9%. The optimal formulation of coconut shell charcoal extract gel is a gel with a concentration of 9%, and the results of the one-way ANOVA statistical analysis show that the treatment of the inhibition test on K. pneumoniae bacteria from gels with different concentrations shows that there is a mean difference in the area of the inhibition zone with a significant value of 0.033 (p<0.05). The results of the LSD post-hoc test showed that several treatment groups had significant values (p <0.05). Based on the results of the study, it can be concluded that the optimal formulation of shell charcoal extract gel is a gel with a concentration of 9%, while the most effective gel for inhibiting the growth of K. pneumoniae bacteria is a gel with a concentration of 3%.

Optimasi Suhu Annealing Gen Blaz Dari Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) Pada Peralatan Medis

2023-11-11T04:41:31+00:00

Methicillin-resistant Staphylococcus aureus is a strain of S. aureus bacteria that is resistant to methicillin-type penicillins. MRSA bacteria can cause nosocomial infections, which can be spread through medical equipment. One of the genes that encodes the resistance trait of S. aureus is the blaZ gene. The detection of the blaZ gene requires optimizing the annealing temperature of the primer used to obtain the optimal PCR band. This study aims to optimize the annealing temperature for the amplification of the blaZ gene DNA from Methicillin-resistant Staphylococcus aureus (MRSA) bacteria on medical devices. The method used in this study used a kit for bacterial extraction and Polymerase Chain Reaction(PCR). The results of the blaZ gene DNA amplification in all samples showed clear bands at temperatures of 52.0°C, 54.2°C, 58.7°C, 61.8°C, and 64.0°C; no smears were formed, and the electrophoretic visualization results on the PCR product showed the results of bands that were according to the target, namely 173 bp. The most optimal annealing temperature for amplification of the blaZ gene in MRSA bacteria from hospital medical equipment is 61.8°C.

Hubungan Tingkat Pengetahuan Ibu Dan Usia Pemberian Mp-Asi Dengan Kejadian Diare Pada Balita Wilayah Kerja Puskesmas Tampa Kabupaten Barito Timur

2023-11-11T04:48:44+00:00

Diarrhea is defined as a digestive disorder that has the characteristics of defecation 3 times or more during one day which produces a watery stool consistency and can be followed by blood. diarrhea cases that occurred in the East Barito area in 2019 were around 3,428 people for all ages and 1,584 toddlers. maternal knowledge and age of complementary feeding is one of the factors that cause diarrhea. The purpose of the study was to determine the relationship between the level of maternal knowledge and the age of complementary feeding with the incidence of diarrhea in toddlers. This research method is a cross sectional analysis with purposive sampling technique totaling 100 samples. Data were obtained through questionnaires. The results showed a percentage distribution of good knowledge level of 75%. The percentage of the age of giving MP-ASI is exactly 76%. Maternal knowledge and the incidence of diarrhea have a significant relationship with a p value of 0.001. Age of complementary feeding and the incidence of diarrhea have a significant relationship with a p value of 0.001. It is hoped that this research can be an evaluation material in health services.

Hubungan Faktor Risiko Usia Dengan Angka Kejadian Kanker Payudara Dan Tumor Jinak Payudara Di RSUD Wangaya Kota Denpasar Tahun 2019-2022

2023-11-11T07:28:58+00:00

Breast cancer is a condition in which there is a loss of control and normal mechanisms in cells, which causes abnormal, uncontrolled, and rapid growth in breast/mammae tissue. One of the risk factors for the occurrence of breast cancer is age, where a person's risk of being diagnosed with breast cancer will continue to increase as a person gets older and peaks at the age of 80 years. In this study to analyze the correlation between age risk factors on the incidence of breast cancer and benign breast tumors. Analytical observational study with a cross-sectional design. The population is all medical record data for the diagnosis of breast cancer and benign breast tumors at the Wangaya Regional General Hospital, Denpasar City in January 2019 – December 2022. Samples were taken using a categorical descriptive method and analyzed using Chi-square. The highest frequency of breast cancer patients was at the age of 46 - 55 years, amounting to 20 patients (64.5%). The highest frequency of patients with benign breast tumors was at the age of 26-35 years, amounting to 24 patients (30.76%). Chi-square test results show that the probability value (p) <0.05 (p = 0.000). There is a significant correlation between age risk factors and the incidence of breast cancer and benign breast tumors at the Wangaya Regional General Hospital, Denpasar City.

Literature Review : Gambaran Efektivitas Pemeriksaan Keracunan Merkuri Dengan Sampel Biologis

2023-11-11T07:36:36+00:00

Mercury is one of the heavy metals with high toxicity and is included in the class of neurotoxic substances that can cause various damage to tissue and nerve cells. The proper use of biomarkers in blood, urine and hair can determine the effectiveness of the mercury poisoning examination in the laboratory. Purpose: This study aimed to describe the effectiveness of mercury poisoning examination using biological samples. Method: This study used a literature review with online database sources from the search engine Science Direct, PubMed, Google Scholar. Result, The cause of mercury poisoning was through the use of mercury in various industrial fields, mining activities, consumption of fish, shellfish and water that had been contaminated with mercury. Examination of mercury poisoning with blood and urine biomarkers was the main choice for acute exposure due to the direct use of mercury, especially in the work environment. Hair biomarkers were the main choice for chronic exposure, mainly fish consumption. The length of working time and the frequency of fish consumption had a significant influence on the high levels of mercury in the body, where the longer and more mercury exposure, the more risk a person was to experience mercury poisoning. Mercury levels in the blood can be detected within 2-3 days, in urine one week after exposure, and in hair, levels are persistent in the long term. Conclusion: The effectiveness of mercury poisoning examination using biological samples depends on the type and duration of exposure, where short-term exposure to high concentrations uses blood and urine biomarkers and long-term exposure using hair biomarkers.

Jamur Kontaminan Pada Nasi Berdasarkan Cara Penyimpanan

2023-11-11T07:46:34+00:00

Rice is a staple food for the people of Indonesia as a source carbohydrate. Improper rice storage can lead to contamination microorganisms. One of these microorganisms is fungus. Contaminant mold is an example of a type of fungus that can grow on foodstuffs such as rice. This study aims to determine the description of fungal contaminants in rice with various kinds of storage, namely rice cooker on, rice cooker off, and serving lid with storage time of 8 hours, 16 hours, and respectively 24 hours. This type of research is a descriptive observational approach cross sectional. The sample uses 3 brands of rice taken from the market Segiri Kota Samarinda with purposive sampling technique. Rice is processed then stored in various places and storage times. After that identification of contaminants macroscopically and microscopically. Data analyzed using univariate analysis. The results of the research show that there is 3 species of contaminating fungi namely Aspergillus sp, Muchor sp, and Penicillium sp. Based on the sample storage with rice cooker on, rice cooker off, and serving cap respectively indicate the percentage growth of the species the same fungus, namely 33.3%.

Gambaran Kadar Hemoglobin Pada Petani Buah Jeruk Pengguna Pestisida Di Desa Karangwidoro Kecamatan Dau Kabupaten Malang

2023-11-11T07:53:50+00:00

Hemoglobin is a protein that contains iron and is found in erythrocytes which functions as a carrier of oxygen from the lungs throughout the body. Hemoglobin also carries carbon dioxide back to the lungs to be exhaled out of the body. When the level of hemoglobin in the body is low, anemia is indicated. Low hemoglobin levels can be caused by the use of pesticides which are toxic chemicals and are often used by farmers to eradicate pests in the agricultural sector. Exposure to pesticides in the body causes the formation of sulfhemoglobin and methemoglobin binding which is the cause of the low amount of hemoglobin in red blood cells resulting in anemia. The purpose of this study was to describe the level of hemoglobin in citrus fruit farmers using pesticides in Karangwidoro Village, Dau District, Malang Regency. This research is a descriptive research with purposive sampling technique. The population is 76 farmers and the sample consists of 22 male farmers. Hemoglobin levels were checked using the Easy Touch Hemoglobin tool. The normal value of hemoglobin level is 13.0 g/dL-17.0 g/dL. The results showed that Hemoglobin levels in citrus fruit farmers using pesticides ranged on average 14.9 g/dL, the lowest value was 11.5 g/dL, and the highest value was 18.0 g/dL. The conclusion in this study is that almost all farmers who use pesticides have hemoglobin levels within normal limits. The suggestion for this research is to be able to add a series of complete blood profile examinations such as erythrocytes, hematocrit, leukocytes, and platelets.

Hubungan Antara Kadar Magnesium Serum Dengan HbA1c Pada Penderita Diabetes Mellitus Tipe 2 Di RSUP Fatmawati Jakarta

2023-11-11T07:59:51+00:00

Diabetes Mellitus (DM) is a collection of symptoms that arise in a person caused by an increase in blood glucose levels due to a progressive decrease in insulin secretion. magnesium (Mg) plays an important role in insulin secretion, insulin binding, and homeostasis. Mg Serum level is an important factor in the etiology and development of macrovascular and microvascular complications in diabetes mellitus patients. HbA1c in DM patients is used to monitor glycemic control and predict the progression of diabetes complications. The purpose of this study was to determine the relationship between serum magnesium and HbA1c levels in patients with type 2 diabetes mellitus. The design of this study was an analytical observational study with a cross-sectional design. The data used are secondary data obtained from patients with type 2 diabetes mellitus at Fatmawati General Hospital for the period 2019 – 2020. The research sample used was 84 data for patients with type 2 diabetes mellitus. Analysis of the data used includes descriptive analysis and then hypothesis testing using the Spearman correlation test. The result of this study showed an association between serum magnesium and HbA1c levels with a value of p = 0,000 (p ≤ 0,05) and r = 0,513. The conclusion obtained in this study is that there is a strong negative pattern relationship between serum magnesium and HbA1c levels in type 2 diabetes mellitus.

Perbedaan Hasil Pewarnaan Hematoxylin Eosin (HE) Pada Histologi Kulit Mencit (Mus Musculus) Berdasarkan Ketebalan Pemotongan 3 Μm, 6 Μm Dan 9 Μm

2023-11-11T08:05:43+00:00

Sectioning is a step that must be passed before staining Hematoxylin Eosin (HE). The aim of this study was to determine differences in the results of Hematoxylin Eosin (HE) staining in mice skin histology (Mus musculus) based on the thickness of the microtome sections of 3 μm, 6 μm, and 9 μm. Experimental research, true experimental research design post test only control group design. The research sample was mice skin preparations (Mus musculus). Primary data collection, cutting of skin preparations using a microtome and staining Hematoxylin Eosin (HE). Field readings were carried out at 400x magnification (40x objective). Data was processed using Kruskal Wallis and Man Whitney. The average value of 6 μm nuclear cutting was 1.44, cytoplasm was 1.15 and color uniformity was 3. The results showed an abnormal distribution, the Kruskal Wallis test (p=0.008) there was a difference in the quality of staining in the 3 μm, 6 μm and 9 μm. The Man Whitney test for the 3 μm and 6 μm microtome cutting groups (p=0.412) showed no difference in the group, the 6 μm and 9 μm microtome cutting groups (p=0.004) there were differences in the group, the 3 μm and 9 μm cutting groups ( p=0.004) there was a difference in the cutting group. The conclusion of this study was that 6 μm microtome cutting produced preparations with better staining quality compared to 3 μm and 9 μm microtome cutting.