@article{Ma’arif_Muslikh_Amalia_Mahardiani_Muchlasi_Riwanti_Taek_Laswati_Agil_2022, place={Palangka Raya, Indonesia}, title={Metabolite Profiling of the Environmental-Controlled Growth of Marsilea crenata Presl. and Its In Vitro and In Silico Antineuroinflammatory Properties}, volume={5}, url={https://journal.umpr.ac.id/index.php/bjop/article/view/3262}, DOI={10.33084/bjop.v5i3.3262}, abstractNote={<p style="text-align: justify;"><span data-preserver-spaces="true">This study was aimed to evaluate the metabolite contents and antineuroinflammatory potential of </span><em><span data-preserver-spaces="true">Marsilea crenata</span></em><span data-preserver-spaces="true"> Presl. grown under a controlled environmental condition. The antineuroinflammatory test has been carried out </span><em><span data-preserver-spaces="true">in vitro</span></em><span data-preserver-spaces="true"> using ethanolic extract of </span><em><span data-preserver-spaces="true">M. crenata</span></em><span data-preserver-spaces="true"> leaves on HMC3 microglia cells. An <em>in silico</em> approach was applied to predict the active compounds of the extract. The HMC3 microglia cells were induced with IFNγ to create prolonged inflammatory conditions and then treated with 96% ethanolic extract of the </span><em><span data-preserver-spaces="true">M. crenata</span></em><span data-preserver-spaces="true"> leaves of 62.5, 125, and 250 μg/mL. The expression of MHC II was analyzed using the ICC method with the CLSM instrument. Metabolites of the extract were profiled using UPLC-QToF-MS/MS instrument and MassLynx 4.1 software. </span><em><span data-preserver-spaces="true">In silico</span></em><span data-preserver-spaces="true"> evaluation was conducted with molecular docking on 3OLS protein using PyRx 0.8 software, and physicochemical properties of the compounds were analyzed using SwissADME webtool. The ethanolic extract of </span><em><span data-preserver-spaces="true">M. crenata</span></em><span data-preserver-spaces="true"> leaves could reduce the MHC II expression in HMC3 microglia cells in all concentrations with the values 97.458, 139.574, and 82.128 AU. The result of metabolite profiling found 79 compounds in the extract. </span><em><span data-preserver-spaces="true">In silico</span></em><span data-preserver-spaces="true"> evaluation showed that 19 compounds gave agonist interaction toward 3OLS, and three met all parameters of physicochemical analysis. The ethanolic extract of the environmental-controlled growth of </span><em><span data-preserver-spaces="true">M. crenata</span></em><span data-preserver-spaces="true"> leaves antineuroinflammatory activity on HMC3 microglia cells. The extract was predicted to contain some phytoestrogen compounds which act as 3OLS agonists.</span></p>}, number={3}, journal={Borneo Journal of Pharmacy}, author={Ma’arif, Burhan and Muslikh, Faisal Akhmal and Amalia, Dilla and Mahardiani, Anisah and Muchlasi, Luthfi Achmad and Riwanti, Pramudita and Taek, Maximus Markus and Laswati, Hening and Agil, Mangestuti}, year={2022}, month={Aug.}, pages={209–228} }