Introduksi Teknologi DNA Barcoding kepada Staf UPTD Perikanan Budidaya Air Payau dan Laut Karang Intan

Introduction of DNA Barcoding Technology to UPTD Staff of Brackish and Marine Aquaculture Karang Intan

Authors

DOI:

https://doi.org/10.33084/pengabdianmu.v9i4.7056

Keywords:

DNA, Barcoding, Fish

Abstract

Partners in community service activity are UPTD Brackish and Marine Aquaculture, Karang Intan, one of the centers under South Kalimantan Province that specializes in the hatchery of local fish such as snakehead (Channa striata), common carp (Cyprinus carpio), and climbing perch (Anabas testudineus). The problem in the partner is fish farming that still relies on seeds from nature, which causes several problems, such as slow seed growth, decreased production quality, disease attacks and hereditary abnormalities that are getting higher due to interbreeding. Based on these problems, the team collaborated with the UPTD for Brackish and Marine Aquaculture, Karang Intan, to improve the genetic quality of fish by introducing DNA barcoding techniques, especially at the spawning stage. The introduction of DNA barcoding technology is carried out through (1) classical counseling and group discussions, 2) training and assistance covering technical aspects of DNA barcoding (fish DNA isolation, DNA amplification, visualization of PCR products with electrophoresis, to the sequencing). Based on the evaluation, 100% of the participants were enthusiastic and realized the importance of DNA barcoding techniques to produce superior broodstock and fish fry, especially for snakehead, common carp, and climbing perch commodities.

Downloads

Download data is not yet available.

References

Hebert, P. D., Ratnasingham, S., & deWaard, J. R. 2003. Barcoding Animal Life: Cytochrome c Oxidase Subunit 1 Divergences among Closely Related Species. Proceedings Biological Sciences, 270(1):S96–S99. https://doi.org/10.1098/rsbl.2003.0025.

Harada, A. E., Lindgren, E. A., Hermsmeier, M. C., Rogowski, P. A., Terrill, E., Burton, R. S., & Bernardi, G. 2015. Monitoring Spawning Activity in a Southern California Marine Protected Area using Molecular Identification of Fish Eggs. PLoS ONE, 10(8):1–21. https://doi.org/10.1371/journal.pone.0134647.

Hofmann, T., Knebelsberger, T., Kloppmann, M., Ulleweit, J., & Raupach, M. J. 2017. Egg Identification of Three Economical Important Fish Species using DNA Barcoding in Comparison to a Morphological Determination. Journal of Applied Ichthyology, 33(5):925–932. https://doi.org/10.1111/jai.13389.

Panprommin, D., Soontornprasit, K., Tuncharoen, S., & Iamchuen, N. 2021. Efficacy of DNA Barcoding for the Identification of Larval Fish Species in the Upper and Middle Ing River, Thailand. Gene Reports, 23(12): 101057. https://doi.org/10.1016/j.genrep.2021.101057.

Rahayu, D, Q., Nugroho, E, D., & Listyorini, D. (2019). DNA Barcoding Ikan Introduksi Khas Telaga Sari, Kabupaten Pasuruan. Biotropika Journal of Tropical Biology 7(2):51-62 . http://dx.doi.org/10.21776/ub.biotropika.2019.007.02.2

Saleky, D., Sianturi, R., Dailami, M., & Kusuma, A. B. 2021. Kajian Molekuler Ikan Oreochromis spp. dari Perairan Daratan Merauke-Papua, Berdasarkan DNA Mitokondria Fragmen Gen Sitokrom Oksidase Subunit I. Jurnal Perikanan Universitas Gadjah Mada, 23(1):37. https://doi.org/10.22146/jfs.61026.

Suryaningtyas, T. I. 2017. Aplikasi Bioteknologi Molekuler dalam Budidaya Perairan. Oseana, 42(4):13–24. https://doi.org/10.14203/oseana.2017.Vol.42No.4.70.

Victor, B. C., Hanner, R., Shivji, M., Hyde, J., & Caldow, C. 2009. Identification of the Larval and Juvenile Stages of the Cubera Snapper, Lutjanus cyanoptems, using DNA Barcoding. Zootaxa, 36(2215):24–36. https://doi.org/10.11646/zootaxa.2215.1.2.

Downloads

Published

2024-04-30

How to Cite

Sasmita, R., Hidayaturrahmah, H., Badruzsaufari, B., Firnanda, M. R., & Fitri, M. (2024). Introduksi Teknologi DNA Barcoding kepada Staf UPTD Perikanan Budidaya Air Payau dan Laut Karang Intan: Introduction of DNA Barcoding Technology to UPTD Staff of Brackish and Marine Aquaculture Karang Intan. PengabdianMu: Jurnal Ilmiah Pengabdian Kepada Masyarakat, 9(4), 682–687. https://doi.org/10.33084/pengabdianmu.v9i4.7056

Issue

Vol. 9 No. 4 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat

Section

Articles

License

Copyright (c) 2024 Rani Sasmita, Hidayaturrahmah Hidayaturrahmah, Badruzsaufari Badruzsaufari, Muhammad Riyan Firnanda, Muhammad Fitri

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Any article on the copyright is retained by the author(s).
  2. Author grant the journal, right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share work with acknowledgment of the work authors and initial publications in this journal.
  3. Authors are able to enter into a separate, additional contractual arrangements for non-exclusive distribution of published articles of work (eg, post-institutional repository) or publish it in a book, with acknowledgment of its initial publication in this journal.
  4. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to and during the submission process, as can lead to productive exchanges, as well as earlier and greater citation of published work.
  5. The article and any associated published material is distributed under the Creative Commons Attribution-ShareAlike 4.0 International License