Uji Stabilitas Pooled Sera Yang Disimpan Di Suhu 2 - 8ºc Pada Pemeriksaan Albumin
The Pooled Sera Stability Test is Stored at a Temperature of 2 - 8ºc on Albumin
DOI:
https://doi.org/10.33084/bjmlt.v6i1.6073Keywords:
Albumin, Pooled sera, control serum, albumin stabilityAbstract
The health laboratory is one of the health services for the community in helping to establish a diagnosis, monitoring treatment results, and determining the prognosis of a disease. Control serum is very important for assessing performance in the laboratory, including instrument and reagent quality. Some laboratories use pooled serum as control serum for daily examination. Albumin is the most abundant plasma protein in the human body, which is about 55-60% and the total normal serum protein level is 3.8-5.0%. This study aims to test the stability of pooled sera on examination of albumin stored in the refrigerator at a temperature of 2 - 8ºC for 2 weeks. This research method uses observational research methods with precision data analysis techniques (accuracy). The results of the study of examining albumin levels using the Bromcresol Green (BCG) method obtained serum stability results on day 1 to day 5, where the CV value from the albumin level examination entered the maximum CV value on albumin, which was 6. The CV value for the 5th day covers, day 1 to day 2 = 1.99. Day 1 to day 3 = 2.17. Day 1 to day 4 = 4.27. And day 1 to day 5 = 4.84. On day 6 to day 14 and so on, the serum is found to be unstable and the value does not enter the maximum CV value.
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References
Allinson, S.P., Lobo, D.N., dan Stanga, Z., 2001. The Treatment of hypoalbuminaemia. Clinical Nutrition (Edinburgh, Scotland), 20: 275-279
Ahmad, Sabri. 2014. Strategi Belajar Mengajar Mikro Teaching. Ciputat: Quntum Teaching.
Depkes RI. 2004. Pedoman Praktik Laboratorium Yang Benar. Jakarta: Departemen Kesehatan
Depkes RI. 2008. Pedoman Praktik Laboratorium Kesehata Yang Benar. (Good Laboratorium Practice).
Depkes RI. 2015. Penyelenggaraan pemeriksaan laboratorium untuk ibu hamil, bersalin, dan nifas di fasilitas pelayanan kesehatan dan jaringan
pelayanannya.
Eaton D.C. and Pooler J.P, in: Vander’s Renal Physiology, 7th Ed, McGraw Hill Companies Inc. Atlanta, 2009, pp. 77-154.
Fulks M. Stout R. Dola V. 2010, Albumin and All-CauseMortality Risk In Insurance Applicants. Journal of Inasurance Medicine 42:11-17.
Gatta, A., Verardo, A., Bolognesi, M. 2012. Hypoalbuminenia, Internal and Emergency Medicine; 7(2): S193-S199
Gede Arie Wijaya, J. N. (2020). Impresisi Pooled Serum Freeze Dried Yang Tersimpan Pada Suhu -24 ºC Terhadap Parameter Albumin. Jurnal SainHealth Vol. 4 No. 2 Edisi September 2020, 4, 16
Handayati, A., Christyaningsih J., & Rini, T. 2014. Uji Stabilitas Pooled Sera Yang Disimpan Dalam Freezer Untuk Pemantapan Mutu Internal Di Laboratorium Klinik. Jurnal Penelitian Kesehatan, 12(1), 55-60.
Kee J.L., 2017, Uji Laboratorium dalam Pedoman Pemeriksaan Laboratorium dan Diagnostik, Edisi ke-6, Penerbit Buku Kedokteran EGC, Jakarta.
Khafaji Ali AL, dan Webb Andrew R. 2003. Should Albumin Be Used To Correct Hypoalbuminemia In The Critically III. tatm. 5: 392-396
Kesehatan, K. 2010. Peraturan Menteri Kesehatan tentang Laboratorium Klinik, 25 Maret 2010.
Miftahul Ilmiah, L. A. 2014. Metode Bromcresol Green (BCG) Dan Bromcresol Purple (BCP) Pada Sirosis Hati Yang Mendapat Infus Albumin. Vol. 20, No.2 Maret 2014.
Mochamad Rizaldi Sumarto, D. A. (n.d.). Pengaruh Wakru Penyimpanan Terhadap Stabilitas Kadar BUN/Blood Urea Nitrogen Dan Kreatinin Dalam Pooled Sera.
Permenkes. 2013. Pedoman Pelaksanaan Permenkes No. 66 Tahun 2013. Registrasi Penelitian Klinik
Rosita HB. 2013. Pemantapan Mutu Internal Tes Traksi Lipid Dirumah Sakit Pendidikan Universitas Hasanuddin (UNHAS). Makassar. Journal.
Sugiyono. 2018. Metode penelitian kuantitatif / prof. Dr. Sugiyono. PEKANBARU: Alfabeta.
Sukorini, Usi, Nugraha, Dwi Kurniawan, Rizki, Mohammad, & P.J. Bambang Hendriawan, 2010. Pemantapan Mutu Internal Laboratorium Klinik. Bagian Patologi Klinik Fakultas Kedokteran Universitas Gajah Mada & Instalansi Patologi Klinik RSU Dr. Sardjito. Penerbit Kanal Medika dan Alfa Media, Yogyakarta.
Sumarno. 2012. Albumin Ikan Gabus (Snakeheads fish) Dan Kesehatan. Jurnal Ilmiah Agri Bias Vol. 10 No 1 : Juni 2012 60-63, 60-63.
Sutedjo, A. 2007. Mengenal Penyakit Melalui Hasil Pemeriksaan Laboratorium. Jakarta Timur: Amara Book.
Widiya, R. Pengaruh Lama Penyimpanan Pooled Sera Pada Freezer Terhadap Mutu Pemeriksaan Kimia Klinik.
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