Analisis Aktivitas Antibakteri Ekstrak Daun Kelor (Moringa oleifera Lam.) Terhadap Pertumbuhan Pseudomonas aeruginosa
Main Article Content
Abstract
Moringa leaves (Moringa oleifera Lam.) are known to have various health benefits, one of which is their potential as an antibacterial agent. This study aims to activate the antibacterial activity of moringa leaf extract against the growth of Pseudomonas aeruginosa. The test was carried out with five treatments, namely moringa leaf extract with concentrations of 15%, 30%, 50%, positive control, and negative control. The well diffusion method was used in the test, with incubation at 37°C for 24 hours. The diameter of the inhibition zone was measured as an indicator of effectiveness. The results showed that a concentration of 15% did not produce an inhibition zone, while concentrations of 30% and 50% showed an average inhibition zone of 0.87 mm and 2.85 mm, respectively. Based on the CLSI criteria, all results were divided into resistant because the size of the inhibition zone was ≤ 15 mm. Thus, it can be concluded that moringa leaf extract has not shown optimal effectiveness in inhibiting the growth of Pseudomonas aeruginosa..
Downloads
Article Details

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
All rights reserved. This publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording.
References
Donkor ES, Nortey T, Opintan J, Akyeh ML. 2007. Antimicrobial susceptibility of Salmonella typhi and Staphylococcus aureus isolates and the effect of some media on susceptibility testing results. The Internet Journal Microbiology, 4:1 –6
Dzoyem, J. P., Hamamoto, H., Ngameni, B., Ngadjui, B. T., & Sekimizu, K. 2013. Antimicrobial action mechanism of flavonoids from Dorstenia species. Drug Discoveries & Therapeutics. 7(2) : 66–72.
Fauziyah, Rohmatul. (2021). Uji Aktivitas Antibakteri Terhadap Bakteri Pseudomonas aeruginosa serta Uji Toksisitas Ekstrak Daun Kelor (Moringa oleifera lamk) Hasil Sonikasi Dengan Variasi Preparasi Sampel. Skripsi. Program Studi Kimia. Universitas Islam Negeri Maulana Malik Ibrahim. Malang.
Listyorini, D. 2019. Uji Daya Hambat Antibakteri Kombinasi Ekstrak Daun Sirih Merah (Piper crocatum) dan Daun Kelor (Moringa oleifera L.) Terhadap Bakteri Escherichia coli. KTI. STIKES Bakti Husada Mulia Madiun, Madiun.
Nassar, M. S., Hazzah, W. A., & Bakr, W. M. (2019). Evaluation of antibiotic susceptibility test results: how guilty a laboratory could be? Journal of the Egyptian Public Health Association, 1-5.
Oxoid. 2022. CM1135 Brain Heart Infusion. United Kingdom.
Oxoid. 2022. CM0337 Mueller Hinton Agar. United Kingdom.
Oxoid. 2022. CM0003 Nutrient Agar. United Kingdom.
Priono, A., Yanti, N. A. & Darlian, L., 2016. Perbandingan Efektivitas Antibakteri Ekstrak Etanol Daun Kelor (Moringaoleiferalamck.) Dan Ekstrak Daun Kirinyuh (Chromolaenaodorata L.). J. AMPIBI. 1: 1-6.
Rusmita, H. (2017). Uji Daya Hambat Ekstrak Etanol Akar Kelakai (Stenochleaena palustris (Burn. f.) Bedd.) Terhadap Bakteri Escherichia coli. KTI. Universitas Muhammadiyah Palangkaraya. Palangkaraya
Thai, T., Salisbury, B. H. & Zito, P. M., 2022. National Library of Medicine. National Center for Biotechnology Information. Ciprofloxacin. [Online] Available at: https://www.ncbi.nlm.nih.gov/books/NBK535454/#_article-19562_s11_ [Accessed 06 July 2022]
Wardaningrum, R. Y. 2020. Perbandingan Aktivitas Antioksidan Ekstrak Etanol Terpurifikasi Ubi Jalar Ungu (Ipomoea batatas .L) Dengan Vitamin E. Skripsi. Universitas Ngudi Waluyo, Semarang.
Yunita, E., Galuh , D. P. & Lestari, D., 2020. Antibacterial Activity Of Moringa Leaves Extract Against Pseudomonas aeruginosa. Jurnal Ilmiah Farmako Bahari. 11 : 189-195.