Aktivitas Antibakteri Kombinasi Ekstrak Biji Manjakani (Quercus infectoria) Dan Daun Sirih Merah (Piper crocatum) Terhadap Pertumbuhan Bakteri Klebsiella pneumoniae: Antibacterial Activity Combination of Manjakani Seed Extract (Quercus infectoria) and Red Betel Leaf (Piper crocatum) Against the Growth of Klebsiella pneumoniae.

Ayu Ashari; Tiara Dini Harlita; Ganea Qorry Aina

doi:10.33084/bjmlt.v6i1.6075

Authors

Ayu Ashari Politeknik Kesehatan Kementerian Kesehatan Kalimantan Timur
Tiara Dini Harlita Politeknik Kesehatan Kementerian Kesehatan Kalimantan Timur
Ganea Qorry Aina Politeknik Kesehatan Kementerian Kesehatan Kalimantan Timur

DOI:

https://doi.org/10.33084/bjmlt.v6i1.6075

Keywords:

Antibacterial, Ciprofloxacin, Klebsiella pneumoniae, Manjakani Seeds (Quercus infectoria), Red Betel (piper crocatum)

Abstract

Potential plants found in Indonesia that can be used for the treatment of urinary tract infections include: Manjakani seeds (Quercus infectoria) and red betel leaves (Piper crocatum). Manjakani seeds are pharmacologically claimed to have biological activities such as anti-inflammatory, antibacterial and antifungal. Red betel leaf has the potential as an antibacterial in the treatment of infectious diseases. The purpose of this study was to determine the antibacterial activity of the combination of manjakani seed extract and red betel leaf in inhibiting the growth of Klebsiella pneumoniae bacteria. The type of research used istrue experiment study with a completely randomized design (CRD) with three factors, namely the type of solvent n-hexane, ethyl acetate, ethanol 96%, ratio (1:1,1:2,2:1) and concentration (75%,100%) with each treatment being repeated twice. This research was conducted in the microbiology laboratory, Labkes, East Kalimantan. The population in this study is the manjakani plant and red betel plant and tested using the agar diffusion technique method Kirby Bauer with positive control (Ciprofloxacin) and negative control (aquadest). Data analysis used is One Way ANOVA and advanced test methods Duncan with a significance level of 0,05. The results obtained showed that there was an effect of antibacterial activity of Manjakani seed extract and red betel leaf on growth K. pneumoniae. The most effective concentration was found in ethanol 96% extract, (2:1 combination of 100% concentration) of 13,97 mm and an effectiveness of 55,74% with a positive control ciprofloxacin 5µg of 25,06 mm. It can be concluded that the combined extract of manjakani seeds and red betel leaves has antibacterial activity against bacteria Klebsiella pneumoniae,but the potential is still low of ciprofloxacin.

Downloads

Download data is not yet available.

References

Asparinda, I., & Juwitaningsih, T. 2020. Uji Aktivitas Antibakteri dan Uji Toksisitas Fraksi Non Polar Gal Manjakani (Quercus infectoria). Acta Pharm Indo, 8(2), 69–79.

Harlita, T., & Aina, G. 2023. The Antibacterial Activity of Dayak Onion Ethanol Extract (Eleutherine Palmifolia (L.) Merr) and Red Ginger (Zingiber Officinale Rosc Var. Rubrum) on Growth Gi Tract Pathogen Bacteria. Jurnal Analis Medika Biosans (JAMBS), 10(1), 61–69.

Harlita, T., Aina, G., Kartini, R. (2022). Aktivitas Antibakteri Kombinasi Ekstrak Etanol Bawang Dayak (Eleuthrerine palmifolia (L.) dan Jahe Merah (Zingiber officinale Rosc Var. Rubrum) Terhadap Pertumbuhan Salmonella typhy. Sains Medisina, 1(2), 109–117.

Harlita, T. D., Oedjijono, & Asnani, A. 2018. The Antibacterial Activity of Dayak Onion (Eleutherine palmifolia (L.) merr) Towards Pathogenic Bacteria. Tropical Life Sciences Research, 29(2), 39–52. https://doi.org/10.21315/tlsr2018.29.2.4

Himalaya, D. 2017. Pengaruh Pemberian Ekstrak Biji Manjakani (Quercus infectoria Gall) Terhadap Bakteri Vginosis dan Candida Penyebab Keputihan (Leukorrhea). Journal of Midwifery, 5(1), 38–44.

Klau, M. L. C., Indriarini, D., & Nurina, R. L. 2021. Uji Aktivitas Ekstrak Etanol Daun Kemangi (Ocimum Sanctum L.) Terhadap Pertumbuhan Bakteri Escherichia Coli Secara in Vitro. Cendana Medical Journal (CMJ), 9(1), 102–111. https://doi.org/10.35508/cmj.v9i1.4942

Listyorini, D. (2019). Uji Daya Hambat Antibakteri Kombinasi Ekstrak Daun Sirih Merah (Piper crocatum) dan Daun Kelor (Moringa oleifera L.) Terhadap Bakteri Escherichia coli. Karya Tulis Ilmiah. STikes Bhakti Husada Mulia Madiun.

Lubis, A. A. G. (2019). Uji Resistensi Aantibiotika Terhadap Bakteri Penyebab Infeksi Saluran Kemih (ISK) Di RSUP H. Adam Malik Medan. Karya Tulis Ilmiah. Politeknik Kesehatan Kemenkes RI Medan.

Muntasir, Abdulkadir, W. S., Harun, A. I., Priska Ernestina Tenda, Makkasau, Muliyadi, Saksosno, R. Y., Fernandez, S., & Wonga, T. M. (2021). Antibiotik dan Resistensi Antibiotik. Rizmedia Pustaka Indonesia.

Nemin, A. M. (2020). Karakteristik Pasien Infeksi Saluran Kemih Dirumah Sakit Umum Lasinrang Kabupaten Pinrang Provinsi Sulawesi Selatan Tahun 2018-2019. Skripsi. Universitas Hasanuddin, Makassar.

Nurhadi, G. (2015). Pengaruh Konsentrasi Tween 80 Terhadap Stabilitas Fisik Obat Kumur Minyak Atsiri Herba Kemangi (Ocimum americanum L). Skripsi. UIN Syarif Hidayatullah Jakarta.

Pratiwi, D. S. (2013). Kajian Uji Resistensi Dan Sensitivitas Antibiotik Ceftriaxon dan Ciprofloxacin pada Penderita Infeksi Saluran Kemih di RSUP Fatmawati. Skripsi, UIN Syarif Hidayatullah Jakarta.

Rohadi, D., Zamzam, M. Y., & Rachmany, L. S. (2019). Uji Daya Hambat Ekstrak Etanol Daun Sirih Merah (Piper crocatum Ruiz & Pav.) Terhadap Pertumbuhan Bakteri Escherichia coli. Medimiuh, 1(2), 171–178.

Triandini, I. G. A. A. H., Ruqqayah, S., & Astuti, N. L. B. (2018). Jurnal In Vitro Tanaman yang Berpotensi Sebagai Antibiotik Alami Untuk Radang Payudara (Mastitis). Jurnal Sangkareang Mataram, 4(3), 14–17.

Wiladatika, M. (2013). Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Sirih Merah (Piper crocatum Ruiz and Pav.) Dan Siprofloksasin Terhadap Staphylococcus aureus, Pseudomonas aeruginosa, dan Klebsiella pneumoniae Beserta Bioautografinya. Naskah Publikasi. Universitas Muhammadiyah Surakarta.

Yanti, N. (2016). Uji Aktivitas Antifungi Ekstrak Etanol Gal Manjakani (Quercus infectoria) Terhadap Candida albicans. Jurnal Ilmiah Mahasiswa Pendidikan Biologi, 1(1), 1–9. https://medium.com/@arifwicaksanaa/pengertian-use-case-a7e576e1b6bf