Optimasi Konsentrasi Primer Dan Suhu Annealing Dalam Mendeteksi Gen Blaz Pada Bakteri Methicillin Resistant Staphylococcus Aureus Di Udara
Optimization of Primary Concentration and Annealing Temperature in Detecting Blaz Gene in Airborne Methicillin-resistant Staphylococcus aureus Bacteria
DOI:
https://doi.org/10.33084/bjmlt.v6i1.6076Keywords:
Gen blaZ, Konsentrasi Primer, PCR, Suhu AnnealingAbstract
Staphylococcus aureus is a species of bacteria that is often identified as a source of infection and has increased the number of cases of resistance. Resistance to Penicillin-class antibiotics in S. aureus is associated with plasmids containing the blah-Z gene encoding ß-Lactamase. Some factors that can be modified to obtain optimal test results in PCR are annealing temperature and primary concentration. This study aims to identify the primary concentration and annealing temperature that produce PCR products with the best band in detecting the blaZ gene in Metichilin Resistant Staphylococcus aureus (MRSA) bacteria. The research design used used experimental methods. The results of the study obtained were PCR products at primary concentrations of 10 μM were clearly visible, thick and clean, compared to primary concentrations of 5 μM. Annealing temperature with the best band results at temperatures of 61.8 °C and 58.7°C. This study concluded the primary concentration to obtain a clearly visible band result of 10 μM. The annealing temperature of 61.8°C produced the best band in the sample code R.PG. U4, R.V.U2, and R.151.U2 temperatures. While the sample code R.IGD. U5 band results best at annealing temperature 58.7°C.
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Akinola, O.T., Okedigba, I. and Elutade, O.O. (2022) ‘Occurrence, antibiotic susceptibility and resistance genes among Staphylococcus aureus isolated from keypads of automated teller machines (ATM) in a private university, Nigeria’, Scientific African, 15. Available at: https://doi.org/10.1016/j.sciaf.2022.e01111.
Anggisti, L. and Roslim, D.I. (2018) ‘Optimization of annealing temperature for amplification of actin gene in pandan (Pandanus sp)’, Jurnal Dinamika Pertanian, XXXIV, pp. 95–100. Available at: https://journal.uir.ac.id/index.php/dinamikapertanian/article/download/5411/2618/.
Erlin, E. et al. (2020) ‘Deteksi Methicilin Resistant Staphylococcus aureus (MRSA) Sebagai Penyebab Infeksi Nosokomial Pada Alat-Alat di Ruang Perawatan Bedah’, Quagga: Jurnal Pendidikan dan Biologi, 12(2), p. 137. Available at: https://doi.org/10.25134/quagga.v12i2.2671.
Fitri, R.R.A., Farida and Prasetio, E. (2021) ‘Perbandingan Metode PCR Konvensional Dengan Metode PCR Portable Kit Untuk Deteksi Wssv Pada Udang Vannamei’, Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan, 9(1), pp. 54–62. Available at: https://doi.org/10.29406/jr.v9i1.2615.
Halim, S.V. and Setiawan, E. (2020) ‘Seftarolin, Antibiotik Baru dengan Aktivitas Anti-MRSA: Sebuah Kajian Efektivitas, Keamanan, dan Biaya Penggunaan’, Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(1), pp. 160–180. Available at: https://doi.org/10.22487/j24428744.2020.v6.i1.15015.
Herman et al. (2017) ‘Optimasi Suhu Annealing Untuk Primer g-SSR Dan EST-SSR Pada Kacang Hijau (Vigna radiata L .)’, Jurnal Dinamika Pertanian, XXXIII(1), pp. 95–102.
Herman, Nainggolan, M. and Roslim, D.I. (2018) ‘Optimizing Temperature Annealing for Four Primary RAPD in Mung bean ( Vigna radiata L .)’, XXXIV(Yusuf 2010), pp. 41–46.
Hutabarat, M.S. et al. (2021) ‘Aplikasi Teknik Polymerase Chain Reaction (PCR) dalam Mendeteksi Streptococcus pneumoniae Menggunakan Gen Autolysin (LytA) sebagai Faktor Virulensi dari Spesimen Klinis Sputum’, Jurnal Biotek Medisiana Indonesia, 9(2), pp. 90–98. Available at: https://doi.org/10.22435/jbmi.v9i2.4412.
Nariska, H., Lestari, P. and Karima, R. (2022) ‘Optimasi Metode Polymerase Chain Reaction-Restriction Fragment Length Polymorphism(PCR-RFLP) untuk Deteksi Polimorfisme Gen MTHFR (C677T) pada Pasien Leukemia Limfoblastik Akut (LLA)Anak’, pp. 1–13.
Pratiwi, A., Sari, R. and Apridamayanti, P. (2019) ‘Optimasi suhu desain primer gen blaZ resistensi pada bakteri Staphylococcus aureus secara in silico’, Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN, 4(1), pp. 3–12.
Pristianingrum, S. et al. (2021) ‘Deteksi Metichilin Resistance Staphylococcus Aureus (MRSA)’, Jurnal Analis Medika Biosains (JAMBS), 8(1), pp. 7–12.
Setyawati, R. and Zubaidah, S. (2021) ‘Optimasi Konsentrasi Primer dan Suhu Annealing dalam Mendeteksi Gen Leptin pada Sapi Peranakan Ongole (PO) Menggunakan Polymerase Chain Reaction (PCR)’, Indonesian Journal of Laboratory, 4(1), p. 36. Available at: https://doi.org/10.22146/ijl.v4i1.65550.
Suyasa, I.B.O. and Mastra, N. (2020) ‘Gambaran Methicillin Resistant Staphylococcus aureus (MRSA) Pada Petugas Kesehatan RSUD Wangaya Kota Denpasar’, Meditory : The Journal of Medical Laboratory, 8(1), pp. 46–52. Available at: https://doi.org/10.33992/m.v8i1.1074.
Triyaningsih, T. et al. (2022) ‘Optimization of qRT-PCR Annealing Temperature of WRKY45 Gene for Detection of Resistance Genes Against Xanthomonas oryzae pv. oryzae on Black Rice Cempo Ireng’, Jurnal ILMU DASAR, 23(1), p. 23. Available at: https://doi.org/10.19184/jid.v23i1.24181.
Wahyudiningsih, T.S. and Sartika, D. (2020) ‘Gene detection optimization of Stelechocarpus burahol ( Bl .) Hook f . & Th . using direct PCR kit’, 14(2), pp. 95–101.
Wasiati, A.R., Bimantara, A. and Anindita, N.S. (2022) ‘Optimasi Dan Uji Sensitivitas Primer Polymerase Chain Reaction (PCR) Gen RNA-Dependent RNA Polymerase (RDRP) SARS-COV-2 Asal Indonesia’, Jurnal Universitas ’Aisyiyah Yogyakarta [Preprint].
Yuenleni, Y. (2019) ‘Langkah-Langkah Optimasi Pcr’, Indonesian Journal of Laboratory, 1(3), p. 51. Available at: https://doi.org/10.22146/ijl.v1i3.48723.
Yustinadewi, P.D., Yustiantara, P.S. and Narayani, I. (2018) ‘Mdr-1 Gene 1199 Variant Primer Design Techniques in Pediatric Patient Buffy Coat Samples With Lla’, Metamorfosa: Journal of Biological Sciences, 5(1), p. 105. Available at: https://doi.org/10.24843/metamorfosa.2018.v05.i01.p16.
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