Characterization of Avocado (Persea americana Mill) Extract Nanoparticles on the Growth of Propionibacterium acne
Main Article Content
Abstract
Propionibacterium acne is one of the bacteria that causes acne. One of the plants that can be used as an alternative herbal medicine is avocado (Persea americana Mill). Nanoparticles are solid colloidal particles with diameter ranging from 1-1000 nm. One of the techniques commonly used in making extract nanoparticles is the ionic gelation technique whisch has benefits such as a simple process, the use of non-organic solvents, and ease of process control. This study aims to compare the effectiveness of avocado fruit extract maceration with extract nanoparticles (Persea americana Mill) on the growth of Propionibacterium acne. Avocado fruit were extracted by maceration and characterization nanoparticle with PSA (Particle Size Analyzer) test and FTIR (Fouerier Transform Infra Red). Finally measure the inhibition zone using the diffusion method. The results showed that the inhibitory power of avocado extract at a concentration of 25% was 16,50 mm, at a concentration of 50% was 13,26 mm, and at a concentration of 75% was 32,27 mm. Meanwhile, avocado extract nanoparticles at concentrations of 25%, 50%, and 75% produced the same inhibitory power, which was 6 mm. The results of statistical analysis using the Friedman nonparametric test showed a value (p<0.05) is 0.000 indicating a significant difference between the inhibition zone of avocado fruit extract and avocado fruit extract nanoparticles.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
All rights reserved. This publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording.
References
Ajizah, A. (2004). Sensitivitas Salmonella typhimurium Terhadap Ekstrak Daun Psidium guajava L. Bioscientiae, 1(1), 31–38. https://doi.org/https://doi.org/10.20527/b.v1i1.130
Akbar, M. R. V., Budiarti, L. Y., & Edyson, E. (2016). Perbandingan Efektivitas Antibakteri Antara Ekstrak Metanol Kulit Batang Kasturi Dengan Ampisilin Terhadap Staphylococcus aureus in Vitro. Berkala Kedokteran, 12(1), 1–9. https://doi.org/10.20527/jbk.v12i1.350
Aristyanti, N. P. P., Wartini, N. M., & Gunam, I. B. W. (2017). Rendemen dan Karakteristik Ekstrak Pewarna Bunga Kenikir (Tagetes erecta L.) Pada Perlakuan Jenis Pelarut dan Lama Ekstraksi. Jurnal Rekayasa Dan Manajemen Agroindustri, 5(3), 13–23. https://ojs.unud.ac.id/index.php/jtip/article/view/34075
Bouarab-Chibane, L., Forquet, V., Lantéri, P., Clément, Y., Léonard-Akkari, L., Oulahal, N., Degraeve, P., & Bordes, C. (2019). Antibacterial Properties of Polyphenols: Characterization and QSAR (Quantitative Structure-Activity Relationship) Models. Frontiers in microbiology, 10, 829. https://doi.org/10.3389/fmicb.2019.00829
Clinical and Laboratory Standards Institute. (2011). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement. CLSI document M100-S21, 31(1). https://www.microrao.com/
Dwitarani, N., Amin, R. R., Sofyah, T. M., Ramadhani, D. N., & Sutoyo, S. (2021). Sintesis dan Karakterisasi Nanoherbal Ekstrak Etanol Kayu Secang (Caesalpinia sappan L.). Jurnal Kimia Riset, 6(2), 102-108. https://doi.org/10.20473/jkr.v6i2.30883
Fahira, N., Rahayu, Y. P., Nasution, H. M., & Nasution, M. P. (2023). Uji Aktivitas Antibakteri Nanopartikel Ekstrak Etanol Daun Matoa (Pometia pinnata J.R. Forst & G. Forst) Terhadap Bakteri Streptococcus mutans. Jurnal Riset Kefarmasian Indonesia, 5(1), 100–119. https://doi.org/10.33759/jrki.v5i1.329
Fitri, D., Kiromah, N. Z. W., & Widiastuti, T. C. (2020). Formulasi Dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Salam (Syzygium polyanthum) Pada Berbagai Variasi Komposisi Kitosan Dengan Metode Gelasi Ionik. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 5(1), 61-69. https://doi.org/10.20961/jpscr.v5i1.39269
Fitriyanti, Norhavid, M. F. R., & Ramadhan, H. (2020). Uji Daya Hambat Ekstrak Etanol 70% Biji Pepaya (Carica papaya L.) Terhadap Bakteri Propionibacterium acnes Penyebab Jerawat. Pharmacoscript, 3(2), 143–149. https://doi.org/10.36423/pharmacoscript.v3i2.400
Hoten, H. V. (2020). Analisis Karakterisasi Serbuk Biokeramik dari Cangkang Telur Ayam Broiler. Jurnal ROTOR, 13(1), 1–10. https://doi.org/10.19184/rotor.v13i1.18874
Julianawati, T., Hendarto, H., & Widjiati. (2020). Penetapan Total Flavonoid, Aktivitas Antioksidan, dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.). Jurnal Penelitian Kesehatan Suara Forikes, 11(1), 49-54. http://dx.doi.org/10.33846/sf11110
Kumar, A., & Mathew, L. (2014). Comparative Account of the Preliminary Phytochemical Aspects of Helicanthes elastica (Desr) Danser Growing on Two Different Hosts. Journal of Pharmacognosy and Phytochemistry, 3(1), 218–221. https://www.phytojournal.com/
Kumowal, S., Fatimawali, & Jayanto, I. (2019). Uji Aktivitas Antibakteri Nanopartikel Ekstrak Lengkuas Putih (Alpinia galanga (L.) Willd) Terhadap Bakteri Klebsiella pneumoniae. Pharmacon, 8(4), 781–790. https://doi.org/10.35799/pha.8.2019.29354
Kurniasari, D., & Atun, S. (2017). Pembuatan dan Karakterisasi Nanopartikel Ekstrak Etanol Temu Kunci (Boesenbergia pandurata) Pada Berbagai Variasi Komposisi Kitosan. Jurnal Sains Dasar, 6(1), 31–35. https://doi.org/10.21831/jsd.v6i1.13610
Lenny, A. A. (2016). Daya Hambat Ekstrak Buah Alpukat (Persea americana Mill) Terhadap Pertumbuhan Staphylococcus aureus dan Staphylococcus epidermidis. In Skripsi. Fakultas Ilmu Keperawatan dan Kesehatan, Universitas Muhammadiyah Semarang : Semarang. http://repository.unimus.ac.id/id/eprint/115
Mailuhu, M., Runtuwene, M. R. J., & Koleangan, H. S. J. (2017). Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Metanol Kulit Batang Soyogik (Saurauia bracteosa DC). Chemistry Progress, 10(1), 1-6. https://doi.org/10.35799/cp.10.1.2017.27967
Marini, Nurnabhilah, E., & Pratiwi, D. (2020). Formulasi dan Evaluasi Sediaan Krim Ekstrak Etanol Buah Alpukat (Persea americana Mill). Journal of Herbs and Farmacological, 2(1), 31–35. https://doi.org/https://doi.org/10.55093/herbapharma.v2i1.123
Monalisa, D. T., Handayani., & Sukmawati, D. 2011. Uji Daya Antibakteri Ekstrak Daun Tapak Liman (Elephantopus sacber L.) Terhadap Staphylococcus aureus dan Salmonella typhi. Jurnal BIOMA, 9(2), 13-20.
Muchyar, D. S. R., Pangemanan, D. H. C., & Supit, A. S. R. (2018). Uji Daya Hambat Perasan Daging Buah Alpukat (Persea americana Mill.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus. Jurnal e-GiGi (eG), 6(1), 34–38. https://doi.org/10.35790/eg.6.1.2018.19653
Noviyani, A. (2022). Review : Potensi Tanaman Alpukat (Persea americana) Sebagai Zat Aktif Dalam Formulasi Sediaan Krim Anti Jerawat. Prosiding Workshop Dan Seminar Nasional Farmasi, 1(1), 371–384. https://doi.org/https://doi.org/10.24843/WSNF.2022.v01.i01.p29
Putriani, K., Permata Dewi, A., Lestari, R., & Ade Syamsuri, N. (2022). Uji Aktivitas Antibakteri Daging Buah Alpukat dan Ekstrak Etanol Daging Buah Alpukat (Persea americana Mill) Terhadap Escherichia coli. Jurnal Ilmiah Farmasi Simplisia, 2(1), 23–29. https://doi.org/10.30867/jifs.v2i1.13
Rachmawaty, F. J., Mahardina, D. A. C., Nirwani, B., Nurmasitoh, T., & Bowo, E. T. (2009). Manfaat Sirih Merah (Piper crocatum) sebagai Agen Anti Bakterial terhadap Bakteri Gram Positif dan Bakteri Gram Negatif. JKKI : Jurnal Kedokteran dan Kesehatan Indonesia, 1(1), 12–20. https://journal.uii.ac.id/JKKI/article/view/543
Rahmah, F. T. (2017). Uji Toksisitas Tanaman Anting-Anting (Acalypha indica Linn) Hasil Ekstraksi Ultrasonikasi dengan Variasi Pelarut dan Lama Ekstraksi. In Skripsi. Fakultas Sains dan Teknologi, Universitas Islam Negeri Maulana Malik Ibrahim Malang: Malang. http://etheses.uin-malang.ac.id/13881/1/14630056
Ramdani, R., & Sibero, H. T. (2015). Treatment for Acne Vulgaris. J Majority, 4(2), 87–95. https://juke.kedokteran.unila.ac.id/index.php/majority/article/view/531
Rumagit, H. M. (2015). Uji Fitokimia dan Uji Aktivitas Antioksidan dari Ekstrak Etanol Spons Lamellodysidea Herbacea. Pharmacon, 4(3), 183–192. https://doi.org/10.35799/pha.4.2015.8858
Sangi, M., Runtuwene, M. R. J., Simbala, H. E. I., & Makang, V. M. A. (2008). Analisis Fitokimia Tumbuhan Obat di Kabupaten Minahasa Utara. Journal Unsrat, 1(1): 47-53. https://doi.org/10.35799/cp.1.1.2008.26
Sari, I. P., Wibowo, M. A., & Arreneuz, S. (2015). Aktivitas Antibakteri Ekstrak Teripang Butoh Keling (Holothuria Leucospilota) dari Pulau Lemukutan terhadap Bakteri Propionibacterium Acnes dan Staphylococcus Epidermidis. Journal JKK, 4(4): 21–28. https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/10868
Saryanti, D., & Nugraheni, D. (2019). Uji Aktivitas Antibakteri Nanopartikel Ekstrak Etanol Daun Sirih (Piper betle Linn) Terhadap Staphylococcus aureus. Prosiding APC (Annual Pharmacy Conference), 4(1), 99–103. https://jurnal.uns.ac.id/apc/article/view/35466/0
Shaikh, J. R., & Patil, M. K. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603-608. https://doi.org/10.22271/chemi.2020.v8.i2i.8834
Siregar, H. N., Rahayu, Y. P., Nasution, H. M., & Nasution, M. P. (2023). Uji Aktivitas Antibakteri Nanopartikel Ekstrak Etanol Daun Matoa (Pometia pinnata J.R. Forst & G. Forst) Terhadap Bakteri Escherichia coli. Jurnal Riset Kefarmasian Indonesia, 5(1), 24–41. https://doi.org/10.33759/jrki.v5i1.329
Suharto, M. A. P., Edy, H. J., & Dumanauw, J. M. (2012). Isolasi dan Identifikasi Senyawa Saponin Dari Ekstrak Metanol Batang Pisang Ambon (Musa var. sapientum L.). Pharmacon, 1(2), 86–92. https://doi.org/10.35799/pha.1.2012.914
Utami, P. 2008. Buku Pintar Tanaman Obat. Jakarta: Agromedia Pustaka.
Venkatachalam, K. (2019). Changes in Phytochemicals and Antioxidant Properties of Kaffir Lime Leaves under Chlling Storage. Khon Kaen Agriculutural Journal, 47 (1), 531-536. https://www.researchgate.net/publication/330845256
Wijaya, H., Jubaidah, S., & Rukayyah, R. (2022). Perbandingan Metode Esktraksi Terhadap Rendemen Ekstrak Batang Turi (Sesbania grandiflora L.) Dengan Menggunakan Metode Maserasi dan Sokhletasi. Indonesian Journal of Pharmacy and Natural Product, 5(1), 1–11. https://doi.org/10.35473/ijpnp.v5i1.1469
Yuliana, B., Suleman, A. W., Alyidrus, R., & Pratiwi, R. I. (2023). Formulasi dan Uji Aktivitas Sediaan Gel Anti Jerawat Ekstrak Etanol Daun Alpukat (Persea americana Mill) Terhadap Propionibacterium acne. Jurnal Ilmiah Jophus : Journal of Pharmacy UMUS, 4(02), 49–56. https://doi.org/https://doi.org/10.46772/jophus.v4i02.899