Telaah Metode Pengujian Aktivitas Antioksidan pada Teh Hijau (Camellia sinensis (L.) Kuntze)
Study of Antioxidan Activity Testing Methods of Green Tea (Camellia sinensis (L.) Kuntze)
DOI:
https://doi.org/10.33084/jsm.v7i1.2024Keywords:
Green tea, Antioxidant activity testing, methods, DPPH, ABTS, FRAP, CUPRAC, ORACAbstract
Green tea (Camellia sinensis (L.) Kuntze) is a trendy herbal drink with many benefits, one as an antioxidant. Catechin from the flavonoid class (polyphenols) in green tea is responsible for determining tea's antioxidant properties. Much research measures antioxidant activity in green tea, so it has to compare the antioxidant activity testing method to see which one method is better based on their mechanism in action. The antioxidant activity testing methods were used are DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS, FRAP (Ferric Reducing Antioxidant Power), CUPRAC (Cupric Reducing Antioxidant Capacity), and ORAC (Oxygen Radical Absorbance Capacity). The results showed that the highest antioxidant activity in green tea was obtained using the ABTS method expressed in Trolox Equivalent, equal to 3210 μmol TE/g with a total phenol of 240.73 mg GAE/g.
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References
1. Fajar, R. I., Wrasiati, L. P., & Suhendra, L. (2018). Kandungan Senyawa Flavonoid Dan Aktivitas Antioksidan Ekstrak Teh Hijau Pada Perlakuan Suhu Awal Dan Lama Penyeduhan. Jurnal Rekayasa Dan Manajemen Agroindustri, 6(3), 196. https://doi.org/10.24843/jrma.2018.v06.i03.p02
2. Malik, A., Ahmad, A. R., & Najib, A. (2013). Daun Teh Hijau Dan Jati Belanda. Jurnal Fitofarmaka Indonesia, 4(2), 238–240.
3. Widyasanti A., Rohdiana D., E. N. (2016).
AKTIVITAS ANTIOKSIDAN EKSTRAK TEH PUTIH (Camellia sinensis) DENGAN METODE DPPH (2,2 Difenil -1- Pikrilhidrazil). 1(1), 1–9.
4. Falah, S. (2016). KETINGGIAN TEMPAT ANTIOXIDANT ACTIVITIES OF GMB 7 VARIETY OF TEA AT DIFFERENT ALTITUDE Balai Penelitian Tanaman Industri dan Penyegar Jalan Raya Pakuwon Km 2 Parungkuda , Sukabumi 43357 Indonesia Fakultas Matematika dan Ilmu Pengetahuan Alam , Departemen. 3, 53–60.
5. Natalia, F., Widyantika, D., Pengajar, S., Teknologi, J., Pertanian, H., Semarang, U., … Semarang, U. (2018). METODE PENYEDUHAN DAN AKTIVITAS ANTIOKSIDATIF MINUMAN TEH ( Camellia sinensis Linn .). 7, 241–249.
6. Nibir, Y. M., Sumit, A. F., Akhand, A. A., Ahsan, N., & Hossain, M. S. (2017). Comparative assessment of total polyphenols, antioxidant and antimicrobial activity of different tea varieties of Bangladesh. Asian Pacific Journal of Tropical Biomedicine, 7(4), 352–357. https://doi.org/10.1016/j.apjtb.2017.01.005
7. Purwanti, L. (2019). PERBANDINGAN AKTIVITAS ANTIOKSIDAN DARI SEDUHAN 3 MERK TEH HITAM (Camellia sinensis (L.) Kuntze) DENGAN METODE SEDUHAN BERDASARKAN SNI 01-1902-1995. Jurnal Ilmiah Farmasi Farmasyifa, 2(1), 19–25. https://doi.org/10.29313/jiff.v2i1.4207
8. Maryam, S., Pratama, R., Effendi, N., & Naid, T. (2016). DENGAN METODE CUPRIC ION REDUCING ANTIOXIDANT CAPACITY Fakultas Farmasi Universitas Muslim Indonesia , Makassar. Jurnal Fitofarmaka Indonesia, 2(1), 90–93.
9. Rachman, S. D., Mukhtari, Z., & Soedjanaatmadja, R. U. M. . (2017). Chimica et Natura Acta. Chimica et Natura Acta, 5(3), 124–131. https://doi.org/10.24198/cna.v6.n1.14791
10. Sardarodiyan, M., & Mohamadi Sani, A. (2016). Natural antioxidants: sources, extraction and application in food systems. Nutrition and Food Science, 46(3), 363–373. https://doi.org/10.1108/NFS-01-2016-0005
11. Juniaty Towaha, B. (2013). KANDUNGAN SENYAWA KIMIA PADA DAUN TEH (Camellia sinensis). 19, 12–16.
12. Sukina B, Gwenny I.P, Suhartati, H. N. (2013). KATEKIN DAUN TEH HIJAU (Camelia sinensis) TERHADAP MALONDIALDEHYDE DAN SUPER OXIDE DISMUTASE. 19, 65–139.
13. Mukty, I., Wirjatmadi, B., & Purwanto, B. (2018). Pengaruh Pemberian Seduhan Teh Hijau ( Camellia Sinensis ) Terhadap Aktivitas Superoksida Dismutase ( SOD ) Effect Of Green Tea ( Camellia Sinensis ) Against Supeoxyde Dismutase ( SOD ) Activity. 2071(September), 158–164.
14. Shabri, & Rohdiana, D. (2016). Optimasi dan Karakterisasi Ekstrak Polifenol Teh Hijau dari Berbagai Pelarut. Jurnal Penelitian Teh Dan Kina, 19(1), 57–66.
15. Lee, L. S., Kim, S. H., Kim, Y. B., & Kim, Y. C. (2014). Quantitative analysis of major constituents in green tea with different plucking periods and their antioxidant activity. Molecules, 19(7), 9173–9186. https://doi.org/10.3390/molecules19079173
16. Peel, O. (2020). Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Daun Teh dan Kulit Jeruk Mandarin. 8(1), 1–7. https://doi.org/10.5281/zenodo.3881551
17. Rahmanisa, S., & Wulandari, R. (2016). Pengaruh Ekstrak Teh Hijau terhadap Penurunan Berat Badan pada Remaja The Effect of Green Tea Extract in Decreasing Weight in Adolescent. Majority, 5(2), 109.
18. Gupta, D. (2015). Methods for determination of antioxidant capacity: A review. International Journal of Pharmaceutical Sciences and Research, 6(2),546–566. https://doi.org/10.13040/IJPSR.09758232.6(2).546-66
19. Xiao, F., Xu, T., Lu, B., & Liu, R. (2020). Guidelines for antioxidant assays for food components. Food Frontiers, 1(1), 60–69. https://doi.org/10.1002/fft2.10
20. Pisoschi, A. M., Pop, A., Cimpeanu, C., & Predoi, G. (2016). Antioxidant capacity determination in plants and plant-derived products: A review. Oxidative Medicine and Cellular Longevity, 2016.
https://doi.org/10.1155/2016/9130976
21. Apak, R., Gorinstein, S., Böhm, V., Schaich, K. M., Özyürek, M., & Güçlü, K. (2013). Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC technical report). Pure and Applied Chemistry, 85(5), 957–998. https://doi.org/10.1351/PAC-REP-12-07-15
22. Molyneux, P. (2004). The use of the stable free radical diphenylpicryl- hydrazyl ( DPPH ) for estimating antioxidant activity. 50(December 2003), 211–219
23. Ácsová, A., Martiniaková, S., & Hojerová, J. (2020). Selectedin vitro methods to determine antioxidant activity of hydrophilic/lipophilic substances. Acta Chimica Slovaca, 12(2), 200–211. https://doi.org/10.2478/acs-2019-0028
24. Oliveira, S ; Souza, G.A; Eckert, C.R; Silva, T.A; Edmar Silva Sobra, E.S; Fávero, O.P; Ferreira, M.J.P; Romoff, P; Baader, W. . (2014). EVALUATION OF ANTIRADICAL ASSAYS USED IN DETERMINING THE ANTIOXIDANT CAPACITY OF PURE Artigo. Quim. Nova, 37(3), 497–503.
25. Windiawati, Bina Lohita Sari, dan S. W. (2015). AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL TEH PUTIH (Camellia sinensis L.) DAN BENALU TEH (Scurulla atropurpurea BL.Dans). 1–8.
26. Shalaby1, E. A., & * and Sanaa M. M. Shanab. (2014). African Journal of Pharmacy and Pharmacology Surfactants solubility, concentration and the other formulations effects on the drug release rate from a controlled-release matrix. 8(13), 364–371. https://doi.org/10.5897/AJPP2013
27. Sukweenadhi, J., Yunita, O., Setiawan, F., Siagian, M. T., Danduru, A. P., & Avanti, C. (2020). Antioxidant activity screening of seven Indonesian herbal extract. 21(5), 2062–2067. https://doi.org/10.13057/biodiv/d210532
28. Veljković, J. N., Pavlović, A. N., Mitić, S. S., Tošić, S. B., Stojanović, G. S., Kaličanin, B. M., … Brcanović, J. M. (2013). Evaluation of individual phenolic compounds and antioxidant properties of black, green, herbal and fruit tea infusions consumed in Serbia: Spectrophotometrical and electrochemical approaches. Journal of Food and Nutrition Research, 52(1), 12–24.
29. Dontha, S. (2016). A review on antioxidant methods. Asian Journal of Pharmaceutical and Clinical Research,9(2),14–32. https://doi.org/10.22159/ajpcr.2016.v9s2.13092
30. Pires, M. A., Munekata, P. E. S., Villanueva, N. D. M., Tonin, F. G., Baldin, J. C., Rocha, Y. J. P., … Trindade, M. A. (2017). The Antioxidant Capacity of Rosemary and Green Tea Extracts to Replace the Carcinogenic Antioxidant ( BHA ) in Chicken Burgers. 2017.
31. Choirunnisa, A. R., Fidrianny, I., & Ruslan, K. (2016). Comparison of Five Antioxidant Assays for Estimating Antioxidant Capacity from Three Solanum SP. Extracts. Asian Journal of Pharmaceutical and Clinical Research, 9, 123–128. https://doi.org/10.22159/ajpcr.2016.v9s2.13155
32. Jayanthi, P., & Lalitha, P. (2011). Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. International Journal of Pharmacy and Pharmaceutical Sciences, 3(SUPPL. 3), 126–128.
33. Pekal, A., Drozdz, P., & Pyrzynska, K. (2012). Comparison of the antioxidant properties of commonly consumed commercial teas. International Journal of Food Properties, 15(5), 1101–1109. https://doi.org/10.1080/10942912.2010.514642
34. Gülçin, I. (2012). Antioxidant activity of food constituents: An overview. Archives of Toxicology, 86(3), 345–391. https://doi.org/10.1007/s00204-011-0774-2
35. Anand Jigisha, Rai Nishant*, K. N. and G. P. (2012). Green tea : A magical herb with miraculous outcomes Review Article GREEN TEA : A MAGICAL HERB WITH MIRACULOUS OUTCOMES Anand Jigisha , Rai Nishant *, Kumar Navin and Gautam Pankaj. 3(May), 139–148.
36. Fryda Amalia, Nana Sutisna Achyadi, A. D. S. (2016). PENGARUH GRADE TEH HIJAU DAN KONSENTRASI GULA STEVIA (Stevia rebaudiana bertoni M.) TERHADAP KARAKTERISTIK SIRUP TEH HIJAU (GREEN TEA). 1–12.
37. Haryato Susilo1, Dwi Indriati2, A. R. (2012). 1 , 2 , 3 1. 2(2), 126–136.
38. Ramiréz-Aristizabal, L. S., Ortiz, A., & Ospina-Ocampo, L. F. (2015). Evaluation of the antioxidant capacity and characterization of phenolic compounds obtained from tea (Camellia sinensis) for products of different brands sold in Colombia. Pharmacologyonline, 3(2015-DECEMBER), 149–159.
2. Malik, A., Ahmad, A. R., & Najib, A. (2013). Daun Teh Hijau Dan Jati Belanda. Jurnal Fitofarmaka Indonesia, 4(2), 238–240.
3. Widyasanti A., Rohdiana D., E. N. (2016).
AKTIVITAS ANTIOKSIDAN EKSTRAK TEH PUTIH (Camellia sinensis) DENGAN METODE DPPH (2,2 Difenil -1- Pikrilhidrazil). 1(1), 1–9.
4. Falah, S. (2016). KETINGGIAN TEMPAT ANTIOXIDANT ACTIVITIES OF GMB 7 VARIETY OF TEA AT DIFFERENT ALTITUDE Balai Penelitian Tanaman Industri dan Penyegar Jalan Raya Pakuwon Km 2 Parungkuda , Sukabumi 43357 Indonesia Fakultas Matematika dan Ilmu Pengetahuan Alam , Departemen. 3, 53–60.
5. Natalia, F., Widyantika, D., Pengajar, S., Teknologi, J., Pertanian, H., Semarang, U., … Semarang, U. (2018). METODE PENYEDUHAN DAN AKTIVITAS ANTIOKSIDATIF MINUMAN TEH ( Camellia sinensis Linn .). 7, 241–249.
6. Nibir, Y. M., Sumit, A. F., Akhand, A. A., Ahsan, N., & Hossain, M. S. (2017). Comparative assessment of total polyphenols, antioxidant and antimicrobial activity of different tea varieties of Bangladesh. Asian Pacific Journal of Tropical Biomedicine, 7(4), 352–357. https://doi.org/10.1016/j.apjtb.2017.01.005
7. Purwanti, L. (2019). PERBANDINGAN AKTIVITAS ANTIOKSIDAN DARI SEDUHAN 3 MERK TEH HITAM (Camellia sinensis (L.) Kuntze) DENGAN METODE SEDUHAN BERDASARKAN SNI 01-1902-1995. Jurnal Ilmiah Farmasi Farmasyifa, 2(1), 19–25. https://doi.org/10.29313/jiff.v2i1.4207
8. Maryam, S., Pratama, R., Effendi, N., & Naid, T. (2016). DENGAN METODE CUPRIC ION REDUCING ANTIOXIDANT CAPACITY Fakultas Farmasi Universitas Muslim Indonesia , Makassar. Jurnal Fitofarmaka Indonesia, 2(1), 90–93.
9. Rachman, S. D., Mukhtari, Z., & Soedjanaatmadja, R. U. M. . (2017). Chimica et Natura Acta. Chimica et Natura Acta, 5(3), 124–131. https://doi.org/10.24198/cna.v6.n1.14791
10. Sardarodiyan, M., & Mohamadi Sani, A. (2016). Natural antioxidants: sources, extraction and application in food systems. Nutrition and Food Science, 46(3), 363–373. https://doi.org/10.1108/NFS-01-2016-0005
11. Juniaty Towaha, B. (2013). KANDUNGAN SENYAWA KIMIA PADA DAUN TEH (Camellia sinensis). 19, 12–16.
12. Sukina B, Gwenny I.P, Suhartati, H. N. (2013). KATEKIN DAUN TEH HIJAU (Camelia sinensis) TERHADAP MALONDIALDEHYDE DAN SUPER OXIDE DISMUTASE. 19, 65–139.
13. Mukty, I., Wirjatmadi, B., & Purwanto, B. (2018). Pengaruh Pemberian Seduhan Teh Hijau ( Camellia Sinensis ) Terhadap Aktivitas Superoksida Dismutase ( SOD ) Effect Of Green Tea ( Camellia Sinensis ) Against Supeoxyde Dismutase ( SOD ) Activity. 2071(September), 158–164.
14. Shabri, & Rohdiana, D. (2016). Optimasi dan Karakterisasi Ekstrak Polifenol Teh Hijau dari Berbagai Pelarut. Jurnal Penelitian Teh Dan Kina, 19(1), 57–66.
15. Lee, L. S., Kim, S. H., Kim, Y. B., & Kim, Y. C. (2014). Quantitative analysis of major constituents in green tea with different plucking periods and their antioxidant activity. Molecules, 19(7), 9173–9186. https://doi.org/10.3390/molecules19079173
16. Peel, O. (2020). Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Daun Teh dan Kulit Jeruk Mandarin. 8(1), 1–7. https://doi.org/10.5281/zenodo.3881551
17. Rahmanisa, S., & Wulandari, R. (2016). Pengaruh Ekstrak Teh Hijau terhadap Penurunan Berat Badan pada Remaja The Effect of Green Tea Extract in Decreasing Weight in Adolescent. Majority, 5(2), 109.
18. Gupta, D. (2015). Methods for determination of antioxidant capacity: A review. International Journal of Pharmaceutical Sciences and Research, 6(2),546–566. https://doi.org/10.13040/IJPSR.09758232.6(2).546-66
19. Xiao, F., Xu, T., Lu, B., & Liu, R. (2020). Guidelines for antioxidant assays for food components. Food Frontiers, 1(1), 60–69. https://doi.org/10.1002/fft2.10
20. Pisoschi, A. M., Pop, A., Cimpeanu, C., & Predoi, G. (2016). Antioxidant capacity determination in plants and plant-derived products: A review. Oxidative Medicine and Cellular Longevity, 2016.
https://doi.org/10.1155/2016/9130976
21. Apak, R., Gorinstein, S., Böhm, V., Schaich, K. M., Özyürek, M., & Güçlü, K. (2013). Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC technical report). Pure and Applied Chemistry, 85(5), 957–998. https://doi.org/10.1351/PAC-REP-12-07-15
22. Molyneux, P. (2004). The use of the stable free radical diphenylpicryl- hydrazyl ( DPPH ) for estimating antioxidant activity. 50(December 2003), 211–219
23. Ácsová, A., Martiniaková, S., & Hojerová, J. (2020). Selectedin vitro methods to determine antioxidant activity of hydrophilic/lipophilic substances. Acta Chimica Slovaca, 12(2), 200–211. https://doi.org/10.2478/acs-2019-0028
24. Oliveira, S ; Souza, G.A; Eckert, C.R; Silva, T.A; Edmar Silva Sobra, E.S; Fávero, O.P; Ferreira, M.J.P; Romoff, P; Baader, W. . (2014). EVALUATION OF ANTIRADICAL ASSAYS USED IN DETERMINING THE ANTIOXIDANT CAPACITY OF PURE Artigo. Quim. Nova, 37(3), 497–503.
25. Windiawati, Bina Lohita Sari, dan S. W. (2015). AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL TEH PUTIH (Camellia sinensis L.) DAN BENALU TEH (Scurulla atropurpurea BL.Dans). 1–8.
26. Shalaby1, E. A., & * and Sanaa M. M. Shanab. (2014). African Journal of Pharmacy and Pharmacology Surfactants solubility, concentration and the other formulations effects on the drug release rate from a controlled-release matrix. 8(13), 364–371. https://doi.org/10.5897/AJPP2013
27. Sukweenadhi, J., Yunita, O., Setiawan, F., Siagian, M. T., Danduru, A. P., & Avanti, C. (2020). Antioxidant activity screening of seven Indonesian herbal extract. 21(5), 2062–2067. https://doi.org/10.13057/biodiv/d210532
28. Veljković, J. N., Pavlović, A. N., Mitić, S. S., Tošić, S. B., Stojanović, G. S., Kaličanin, B. M., … Brcanović, J. M. (2013). Evaluation of individual phenolic compounds and antioxidant properties of black, green, herbal and fruit tea infusions consumed in Serbia: Spectrophotometrical and electrochemical approaches. Journal of Food and Nutrition Research, 52(1), 12–24.
29. Dontha, S. (2016). A review on antioxidant methods. Asian Journal of Pharmaceutical and Clinical Research,9(2),14–32. https://doi.org/10.22159/ajpcr.2016.v9s2.13092
30. Pires, M. A., Munekata, P. E. S., Villanueva, N. D. M., Tonin, F. G., Baldin, J. C., Rocha, Y. J. P., … Trindade, M. A. (2017). The Antioxidant Capacity of Rosemary and Green Tea Extracts to Replace the Carcinogenic Antioxidant ( BHA ) in Chicken Burgers. 2017.
31. Choirunnisa, A. R., Fidrianny, I., & Ruslan, K. (2016). Comparison of Five Antioxidant Assays for Estimating Antioxidant Capacity from Three Solanum SP. Extracts. Asian Journal of Pharmaceutical and Clinical Research, 9, 123–128. https://doi.org/10.22159/ajpcr.2016.v9s2.13155
32. Jayanthi, P., & Lalitha, P. (2011). Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. International Journal of Pharmacy and Pharmaceutical Sciences, 3(SUPPL. 3), 126–128.
33. Pekal, A., Drozdz, P., & Pyrzynska, K. (2012). Comparison of the antioxidant properties of commonly consumed commercial teas. International Journal of Food Properties, 15(5), 1101–1109. https://doi.org/10.1080/10942912.2010.514642
34. Gülçin, I. (2012). Antioxidant activity of food constituents: An overview. Archives of Toxicology, 86(3), 345–391. https://doi.org/10.1007/s00204-011-0774-2
35. Anand Jigisha, Rai Nishant*, K. N. and G. P. (2012). Green tea : A magical herb with miraculous outcomes Review Article GREEN TEA : A MAGICAL HERB WITH MIRACULOUS OUTCOMES Anand Jigisha , Rai Nishant *, Kumar Navin and Gautam Pankaj. 3(May), 139–148.
36. Fryda Amalia, Nana Sutisna Achyadi, A. D. S. (2016). PENGARUH GRADE TEH HIJAU DAN KONSENTRASI GULA STEVIA (Stevia rebaudiana bertoni M.) TERHADAP KARAKTERISTIK SIRUP TEH HIJAU (GREEN TEA). 1–12.
37. Haryato Susilo1, Dwi Indriati2, A. R. (2012). 1 , 2 , 3 1. 2(2), 126–136.
38. Ramiréz-Aristizabal, L. S., Ortiz, A., & Ospina-Ocampo, L. F. (2015). Evaluation of the antioxidant capacity and characterization of phenolic compounds obtained from tea (Camellia sinensis) for products of different brands sold in Colombia. Pharmacologyonline, 3(2015-DECEMBER), 149–159.
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Published
2021-08-30
How to Cite
Aryanti, R., Perdana, F., & Syamsudin, R. A. M. R. (2021). Telaah Metode Pengujian Aktivitas Antioksidan pada Teh Hijau (Camellia sinensis (L.) Kuntze): Study of Antioxidan Activity Testing Methods of Green Tea (Camellia sinensis (L.) Kuntze). Jurnal Surya Medika (JSM), 7(1), 15–24. https://doi.org/10.33084/jsm.v7i1.2024
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