Optimasi Purifikasi Darbepoetin Alfa Berdasarkan Metode Kromatografi Penukaran ION dengan Dua Variasi Resin
Optimization of Purification of Darbepoetin Alfa Based on ION Exchange Chromatography Method with Two Variations of Resin
Keywords:EPO, Kromatografi Penukaran ION, Pemurnian
Kidney failure cases are cases that increase every year. This case cannot be considered trivial because it can threaten someone's life. Indeed, kidney failure is not contagious like other diseases and is caused by a bad lifestyle. The number of cases in Indonesia itself has increased quite sharply. For example, in the Riau Islands the number of dialysis patients experienced a sharp jump from 85 people to 700 people and the increase reached 10 times. Darbepoetin (which is EPO which has more sugar) which is also known as hematopoietic, is a glycoprotein compound that controls the process of erythropoiesis (red blood cell production). This hormone is produced in the kidneys, and some is also produced in the liver. The aim of this research is to purify darbepoetin itself and remove several other proteins or interfering impurities so that darbepoetin itself is ready to be produced and help many people who experience kidney failure. The method used was ion exchange chromatography with Toyopearl DEAE 650 M resin. Optimization was then carried out with various variations, especially the resin variations used were Toyopearl DEAE 650 M and GE-DEAE resins. Based on the results, DPO can be purified with Toyopearl resin. After that, purification optimization was carried out and it was found that TOSOH-DEAE resin was indeed a better resin than GE-DEAE.
Brady, Pamlea N dan Megan A. Macnaughtan. (2015). Evaluation of Colorimetric Assaysfor Analyzing Reductively Methylated Proteins: Biases and Mechanistic Insights. Anal Biochem. 2015 December 15; 491: 43–51. Breidbach Andreas, Don H. Catlin, Gary A. Green, Inna Tregub. 2003.
Delanghe, Joris R. Mathieu Bollen and Monique Beullens. (2008). Testing for recombinant erythropoietin. Wiley-Liss, Inc.
Dennison, Thomas J, Julian Smith, Michael P. Hofmann, Charlotte E. Bland, Raj K. Badhan, Ali Al-Khattawi, Afzal R. Mohammed. (2016). Design of Experiments to Study the Impact of Process Parameters on Droplet Size and Development of Non-Invasive Imaging Techniques in Tablet Coating. PLOS ONE | DOI:10.1371/journal.pone.0157267
Detik com. (2016). Kasus Gagal Ginjal. https://health.detik.com/berita-detikhea lth/d-3129326/kasus-gagal-ginjal-naik- 4-kali-lipat-dalam-5-tahun
Durakovic, Benjamin. (2017). Design of Experiments Application, Concepts, Examples: State of the Art. Periodicals of Engineering and Natural Scinces Vol 5, No 3, December 2017, pp. 421‒439
Emanuelsson, Ida. Anna Karin Janson. Katarina Riso. (2008). Characterising of Chromatography Gels for Purification of Erythropoetin. Hogskolan I Boras.
Falck, David, Markus Haberger, Rosina Plomp, Michaela Hook, Patrick Bulau, Manfred Wuhrer & Dietmar Reusch. (2017). Affinity purification of erythropoietin from cell culture supernatant combinated with MALDITOF- MS analysis of erythropoietin N-glycosylation. Scientific Reports | 7: 532.
Farmakoterapi. (2017). Eritropoietin, Hormon Penghasil Sel Darah Merah. http://www.farmakoterapi.com/eritropo ietin-hormon-penghasil-sel-darah-mera h/
Farooqahmed S. Kittura, Elena Arthura, Maikhanh Nguyena,1, Chiu-Yueh Hunga, David C. Saneb, and Jiahua Xiea. (2015). Two-step purification procedure for recombinant human asialoerythropoietin expressed in transgenic plants. Int J Biol Macromol. 2015 January ; 72: 1111–1116.
Garcia, Osnel, Elias Nelson and Leonardo Gordinez. (2013). Improvement in an Ion Exchange Chromatography Proceure to Increase Recovery and Biological Activity of EPO in Preperation for Paharmaceutical Use. Latin America Journal of Pharmacy. 32(6): 852-9 (2013).
Hernandez, Lourdes. Diobel Stewart, Lourdes Zumalacárregui, Daniel Amaro. (2015). Comparison Among Three Anion Exchange Chromatographic Supports to Capture Erythropoietin From Cell Culture Supernatant. 2015 Jun;33(6):642-6
Homkhao Sakullapat Homkhao, Pongsatorn Poopisut & Dyah Wulandari. (2017). SDS Page. Suranaree University of Technology.
Hong, Zhi-Wei Hong,Yu-Chi Yang, Timothy Pan,, Huey-Fen Tzeng, Hua-Wen Fu. (2017). Differential effects of DEAE negative mode chromatography and gel-filtration chromatography on the charge status of Helicobacter pylori neutrophil-activating protein. Plos One https://doi.org/10.1371/journal.pone.01 73632
Huang, Tao, Mian Long and Bo Huo. (2010). Competitive Binding to Cuprous Ions of Protein and BCA in the Bicinchoninic Acid Protein Assay. The Open Biomedical Engineering Journal, 2010, 4, 271-278
Hussain, Maryam T, Neil Forbes and Yvonne Perrie. (2019). Comparative Analysis of Protein Quantification Methods for the Rapid Determination of Protein Loading in Liposomal Formulations. Pharmaceutics 2019, 11, 39
Jan, Sohail, Zabta Khan Shinwari, Malik Ashiq Rabbani, Sabir Hussain Shah, Muhamad Ishaq Ibrahim, Muhammad Ilyas. (2016). Optimization of an efficient SDS-PAGE protocol for rapid protein analysis of Brassica rapa. Journal of Biodiversity and Environmental Sciences (JBES) Vol. 9, No. 2, p. 17-24, 2016
Jawapos. (2017). Kasus Gagal Ginjal di Kepulauan Riau Melonjak Tajam. https://www.jawapos.com/jpg-today/1 0/07/2018/kasus-gagal-ginjal-di-kepula uan-riau-melonjak-tajam/
Jelkman Wolfgang. (2013). Physiology and Pharmacology of Erytropoetin. Transfusion Medicine and Hemotherapy. 2013;40:302-309
Kang Min-Jung, Sang Mi Shin, Hey Hyun Yoo, Oh-Seung Kwon and Changbae Jin. (2010). Characteristics of IEF Patterns and SDS-PAGE Results of Korean EPO Biosimilars. Bull. Korean Chem. Soc. 2010, Vol. 31, No. 9
Kaselvoya Bibiana & Michal Tkac. (2014). Design of Experiments in Truck Company. KVALITA INOVÁCIA PROSPERITA 19/1 – 2015
Khoury, Graziella El Khoury, Basmah Khogeer, Chen Chen, Kheng T Ng, Shaleem I Jacob & Christopher R Lowe1. (2015). Bespoke affinity ligands for the Kittur.
LIPI. (2015). Eritropoetin.
www.biotek.lipi.go.id/publication/berit a/biotek-dalam-berita/1552-erythropoie tin-epo-dari-ragi-dan-barley
Muller, Egbert. (2005). Properties and Characterization of High Capacity Resin for Biochromatographyc. Chem. Eng. 2005. 28, 11.
Ostrihonova, Marta, Jana Adamíková, Tomáš Molnár, Monika Antošová, Milan Polakovič. (2019). Recombinant human erythropoietin separation using a cation-exchange multimodal adsorbent. Acta Chimica Slovaca, Vol. 12, No. 1, 2019, pp. 103—107
Pergande, Melissa R. and Stephanie M. Cologna. (2017). Isoelectric Point Separations of Peptides and Proteins. Proteomes 2017, 5, 4; doi:10.3390/proteomes5010004.
Reichel. C, R. Kulovics, V. Jordan, M. Watzinger, T. Geisendorfer. (2008). SDS-PAGE of recombinant and endogenous erythropoietins: benefits and limitations of the method for application in doping control. Sport und Buch Strauß - Köln 2008.
Republika. (2018). Kasus Gagal Ginjal Meningkat Tajam. https://www.republika.co.id/berita/gaya-hidup/info-sehat/18/05/06/p8ap02399-jumlah-pasien-penyakit-ginjal-kronis-t erus-meningkat.
Rozycki Mirosław, Ewa Chmurzyńska, Ewa Bilska-Zając, Jacek Karamon, Tomasz Cencek. (2018). Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat. J Vet Res 62, 151-159, 2018
Rubiyana, Yana, Adi Santoso, and Irmanida Batubara. (2015). Comparison of Immobilized Metal Affinity Chromatography Ni-NTA and Co-TALON for the Purification of Recombinant Human Erythropoietin. Makara Journal of Science 19/4 (2015) 137-142
Sartika, I Nyoman. Wayan Retayasa, Made Kardana, Ida Bagus Mudita. (2008). Peran Eritropoetin pada Anemia Prematuritas. Sari Pediatri, Vol. 9, No. 6, April 2008
Schagger, Hermann. (2006). Tricine–SDS-PAGE. Nature Publishing Group.
Schmidt Cleber A., Cristiane F. CODEVILLA, Marcio FRONZA, Renata Girardi CASALI & Sérgio L. DALMORA. (2003). Physico-Chemical Characterization and Biological Evaluation of Recombinant Human Erythropoietin in Pharmaceutical Products. Lat. Am. J. Pharm. 22 (4): 343-50 (2003)
Smejkal Gary B and Darren J. Bauer. (2012). High Speed Isoelectric Focusing of Proteins Enabling Rapid Two-Dimensional Gel Electrophoresis. University of New Hampshire
Stanley,William. (2016). Peptide Afinity Adsorbents for Purification of High Value Biotherapics. Disertation. North California State.
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