Cessation of bleeding is influenced by many factors including the type of medication used. The use of drugs to stop bleeding can be done in various ways, one of which is the use of traditional medicine. One of the traditional plants that can be used as a stop bleeding is broccoli (Brassica oleracea L. var. italica) which has a fairly high vitamin K content, where is efficacious as hemostatic. This study aims to determine the effect of broccoli on the bleeding time of mice tail cuts. The first group was negative control (Na-CMC), the second group was positive control (tranexamic acid), while groups three, four, and five were broccoli extract dose of 20, 40, and 60 mg/kg BW, respectively. All treatments were given orally for seven days. Bleeding time was calculated from the initial blood loss until the blood stopped (tail bleeding I method) and the data were analyzed with the ANOVA one way test and the post hoc Least Significant Difference (LSD) statistical test. The results showed that there were differences in the time to stop bleeding in the treatment control group at a dose of 20 mg/kg BW (122.60 ± 29.535 seconds), 40 mg/kg BW (102.40 ± 9.607 seconds) and 60 mg/kg BW (90.40 ± 3.845 seconds). From these results, it can be concluded that the extract of broccoli at a dose of 60 mg/kg BW gives the best results as hemostatic, while the effect is almost similar to the positive control group.
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Batool, R., Khan, M.R., Sajid, M., Ali, S., & Zahra, Z. (2019). Estimation of phytochemical constituents and in vitro antioxidant potencies of Brachychiton populneus (Schott & Endl.) R.Br. BMC Chemistry, 13(1), 32. doi: 10.1186/s13065-019-0549-z
Chauhan, E.S., Tiwari, A., & Singh, A. (2016). Phytochemical screening of red cabbage (Brassica oleracea) powder and juice - A comparative study. Journal of Medicinal Plants Studies, 4(5), 196-199.
Chen, L., Deng, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Li, Y., Wang, X., & Zhao, L. (2018). Inflammatory responses and inflammation-associated diseases in organs. Oncotarget, 9(6), 7204-7218. doi: 10.18632/oncotarget.23208
Curnow, J., Pasalic, L., & Favaloro, E.J. (2016). Why Do Patients Bleed?. The Surgery Journal, 2(1), 29-43. doi: 10.1055/s-0036-1579657
Guo, S. & DiPietro, L.A. (2010). Factors Affecting Wound Healing. Journal of Dental Research, 89(3), 219-229. doi: 10.1177/0022034509359125
Hossain, M.A., Al-Raqmi, K.A.S., Al-Mijizy, Z.H., Weli, A.M., & Al-Riyami, Q. (2013). Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris. Asian Pacific Journal of Tropical Biomedicine, 3(9), 705-710. doi: 10.1016/S2221-1691(13)60142-2
Hussain, M., Debnath, B., Qasim, M., Bamisile, B.S., Islam, W., Hameed, M.S., Wang, L., & Qiu, D. (2019). Role of Saponins in Plant Defense Against Specialist Herbivores. Molecules, 24(11), 2067. doi: 10.3390/molecules24112067
Janarthanan, M. & Kumar, M.S. (2013). Qualitative and Quantitative Analysis of Phytochemical Studies on Selected Seaweeds Acanthopora Spicifera and Sargassum Wightii. International Journal of Engineering Research and Development, 7(3), 11-15.
Levy, J.H., Koster, A., Quinones, Q.J., Milling, T.J., & Key, N.S. (2018). Antifibrinolytic Therapy and Perioperative Considerations. Anesthesiology, 128(3), 657-670. doi: 10.1097/ALN.0000000000001997
Liu, J., Lin, S., Wang, Z., Wang, C., Wang, E., Zhang, Y., & Liu, J. (2012). Pharmacognosy and Pharmacobiotechnology. Maryland, United States: Willimans & Wilkins.
Mageney, V., Neugart, S., & Albach, D.C. (2017). A Guide to the Variability of Flavonoids in Brassica oleracea. Molecules, 22(2), 252. doi: 10.3390/molecules22020252
Panche, A.N., Diwan, A.D., & Chandra, S.R. (2016). Flavonoids: an overview. Journal of Nutritional Science, 5, 47. doi: 10.1017/jns.2016.41
Periayah, M.H., Halim, A.S., & Saad, A.Z.M. (2017). Mechanism Action of Platelets and Crucial Blood Coagulation Pathways in Hemostasis. International Journal of Hematology-Oncology and Stem Cell Research, 11(4), 319-327.
Pierce, A. & Pittet, J.F. (2014). Practical understanding of hemostasis and approach to the bleeding patient in the OR. Advances in Anesthesia, 32(1), 1-21. doi: 10.1016/j.aan.2014.08.009
Raiola, A., Errico, A., Petruk, G., Monti, D.M., Barone, A., & Rigano, M.M. (2017). Bioactive Compounds in Brassicaceae Vegetables with a Role in the Prevention of Chronic Diseases. Molecules, 23(1), 15. doi: 10.3390/molecules23010015
Rishavy, M.A. & Beckner, K.L. (2012). Vitamin K Oxygenation, Glutamate Carboxylation, and Processivity: Defining the Three Critical Facets of Catalysis by the Vitamin K–Dependent Carboxylase. Advances in Nutrition, 3(2), 135-148. doi: 10.3945/an.111.001719
Sacher, R.A. (2012). Tinjauan Klinis Hasil Pemeriksaan Laboratorium. 11th Edition. Jakarta, Indonesia: EGC.
Saghazadeh, S., Rinoldi, C., Schot, M., Kashaf, S.S., Sharifi, F., Jalilian, E., Nuutila, K., Giatsidis, G., Mostafalu, P., Derakhshandeh, H., Yue, K., Swieszkowski, W., Memic, A., Tamayol, A., & Khademhosseini, A. (2018). Drug Delivery Systems and Materials for Wound Healing Applications. Advanced Drug Delivery Reviews, 127, 138-166. doi: 10.1016/j.addr.2018.04.008
Samanta, R., Pattnaik, A.K., Pradhan, K.K., Mehta, B.K., Pattanayak, S.P., & Banerjee, S. (2016). Wound Healing Activity of Silibinin in Mice. Pharmacognosy Research, 8(4), 298-302. doi: 10.4103/0974-8490.188880
Sherwood, L. (2015). Fisiologi Manusia dari Sel ke Sistem. 9th Edition (pp. 430-431). Jakarta, Indonesia: EGC.
United States Department of Agriculture. (2012). Broccoli, Onion, Garlic and Coriander. In National Nutrient Database for Standard Reference (Ch. 26). United States: United States Department of Agriculture Nutrient Data Laboratory and Health.
Xu, Y., Xu, W., Wang, A., Meng, H., Wang, Y., Liu, S., Li, R., Lu, S., & Peng, J. (2019). Diagnosis and treatment of traumatic vascular injury of limbs in military and emergency medicine: A systematic review. Medicine, 98(18), 15406. doi: 10.1097/MD.0000000000015406
Yuan, H., Ma, Q., Ye L., & Piao, G. (2016). The Traditional Medicine and Modern Medicine from Natural Products. Molecules, 21(5), 559. doi: 10.3390/molecules21050559
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