The Combination of Turmeric (Curcuma domestica) Rhizome Extract and Collagen in A Serum Formulation as an Antioxidant
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Submitted
7 August 2020
7 August 2020
Published
28 February 2021
28 February 2021
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Abstract
Turmeric (Curcuma domestica) has nutritious compounds called curcuminoids, which can be used as antioxidants. As an antioxidant, C. domestica extract can be used to ward off free radicals that damage collagen and elastin, a protein that keeps skin moist. This study aimed to determine the antioxidant activity of serum combined with collagen's addition using the DPPH method. The DPPH was made at a concentration of 80 μg/mL, and the absorption was read at a wavelength of 520 nm using a microplate rider. The study was conducted by making six formulations: F0, F1, F2, F3, F4, and F5 obtained the results of serum made from C. domestica extract that could inhibit free radicals and meet the physical evaluation test requirements of serum. Furthermore, the formula was made using only one active ingredient and only collagen to determine the extract or collagen's antioxidant activity. The results obtained indicate that collagen had a supporting role in adding antioxidant activity apart from its extract. The highest % inhibition value at F5 with 90.526% could ward off free radicals.
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References
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23. Maharani BC, Lindriati T, Diniyah N. Pengaruh Variasi Waktu Blanching dan Konsentrasi Asam Sitrat Terhadap Karakteristik dan Aktivitas Ekstrak Pigmen Ubi Jalar Ungu (Ipomoea batatas L.). JP2 Jurnal Penelitian Pangan Indones J Food Res. 2016;1(1):60-7.
24. Hasrawati A, Hardianti H, Qama A, Wais M. Pengembangan Ekstrak Etanol Limbah Biji Pepaya (Carica papaya L.) Sebagai Serum Antijerawat. Jurnal Fitofarmaka Indonesia. 2020;7(1):1-8. doi:10.33096/jffi.v7i1.458
25. Yumas M. Formulasi Sediaan Krim Wajah Berbahan Aktif Ekstra Metanol Biji Kakao Non Fermentasi (Theobroma cacao L) Kombinasi Madu Lebah. Jurnal Industri Hasil Perkebunan. 2016;11(2):75-87. doi:10.33104/jihp.v11i2.3414
26. Directorate General of Drug and Food Control, Ministry of Health, Republic of Indonesia. Formularium Kosmetika Indonesia. Jakarta, Indonesia: Ministry of Health, Republic of Indonesia; 1985.
27. Badan Standarisasi Nasional. Sediaan Tabir Surya. SNI 16-4399-1996. Jakarta, Indonesia: Badan Standarisasi Nasional; 1996.
28. Ardana M, Aeyni V, Ibrahim A. Formulasi dan Optimasi Basis Gel HPMC (Hidroxy Propyl Methyl Cellulose) dengan Berbagai Variasi Konsentrasi. J Trop Pharm Chem. 2015;3(2):101-8. doi:10.25026/jtpc.v3i2.95
29. Tambunan S, Sulaiman TNS. Gel Formulation of Lemongrass Essential Oil with HPMC and Carbopol Bases. Majalah Farmaseutik. 2018;14(2):87-95. doi:10.22146/farmaseutik.v14i2.42598
30. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011;48(4):412-22. doi:10.1007/s13197-011-0251-1
31. Pratiwi D, Wardaniati I. Pengaruh Variasi Perlakuan (Segar dan Simplisia) Rimpang Kunyit (Curcuma domestica) terhadap Aktivitas Antioksidan dan Kadar Fenol Total. Jurnal Farmasi Higea. 2019;11(2):159-65.
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2. Phaniendra A, Jestadi DB, Periyasamy L Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian J Clin Biochem. 2015;30(1):11-26. doi:10.1007/s12291-014-0446-0
3. Silva SAM, Michniak-Kohn B, Leonardi GR. An overview about oxidation in clinical practice of skin aging. An Bras Dermatol. 2017;92(3):367-74. doi:10.1590/abd1806-4841.20175481
4. Poljšak B, Dahmane R. Free Radicals and Extrinsic Skin Aging. Dermatol Res Pract. 2012;2012:135206. doi:10.1155/2012/135206
5. Zhang S, Duan E. Fighting against Skin Aging. The Way from Bench to Bedside. Cell Transplant. 2018;27(5):729-38. doi:10.1177/0963689717725755
6. Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018;13:752-72. doi:10.2147/CIA.S158513
7. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-26. doi:10.4103/0973-7847.70902
8. Garre A, Narda M, Valderas-Martinez P, Piquero J, Granger C. Antiaging effects of a novel facial serum containing L-Ascorbic acid, proteoglycans, and proteoglycan-stimulating tripeptide: ex vivo skin explant studies and in vivo clinical studies in women. Clin Cosmet Investig Dermatol. 2018;11:253-63. doi:10.2147/CCID.S161352
9. Mardhiani YD, Yulianti H, Azhary DP, Rusdiana T. Formulasi dan Stabilitas Sediaan Serum dari Ekstrak Kopi Hijau (Coffea canephora var. Robusta) sebagai Antioksidan. Indones Nat Res Pharm J. 2017;2(2):19-33.
10. Amalraj A, Pius A, Gopi S, Gopi S. Biological activities of curcuminoids, other biomolecules from turmeric and their derivatives – A review. J Trad Complement Med. 2017;7(2):205-33. doi10.1016/j.jtcme.2016.05.005
11. Vaughn AR, Branum A, Sivamani RK. Effects of Turmeric (Curcuma longa) on Skin Health: A Systematic Review of the Clinical Evidence. Phytother Res. 2016;30(8):1243-64. doi:10.1002/ptr.5640
12. Winarsih W, Wintarsih I, Sulistyawati NP, Wahyudina I. Uji Toksisitas Akut Ekstrak Rimpang Kunyit pada Mencit: Kajian Histopatologis Lambung, Hati dan Ginjal. Jurnal Veteriner Jurnal Kedokteran Hewan Indonesia. 2012;13(4):402-9.
13. Hewlings SJ, Kalman DS. Curcumin: A Review of Its’ Effects on Human Health. Foods. 2017;6(10):92. doi:10.3390/foods6100092
14. Aguirre-Cruz G, León-López A, Cruz-Gómez V, Jiménez-Alvarado R, Aguirre-Álvarez G. Collagen Hydrolysates for Skin Protection: Oral Administration and Topical Formulation. Antioxidants. 2020;9(2):181. doi:10.3390/antiox9020181
15. Bolke L, Schlippe G, Gerß J, Voss W. A Collagen Supplement Improves Skin Hydration, Elasticity, Roughness, and Density: Results of a Randomized, Placebo-Controlled, Blind Study. Nutrients. 2019;11(10):2494. doi:10.3390/nu11102494
16. Budiarti E, Budiarti P, Aristri MA, Batubara I. Kolagen dari Limbah Tulang Ayam (Gallus gallus domesticus) terhadap Aktivitas Anti Aging secara In Vitro. Alchemy Jurnal Penelitian Kimia. 2019;15(1):44-56. doi:10.20961/alchemy.15.1.23046.44-56
17. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanarin J Sci Technol. 2004;26(2):211-9.
18. Fitriani L, Saputra F, Melisa M, Zaini E. Studi Awal Sediaan Gel Ekstrak Etanol Kayu Angin (Usnea Sp) untuk Penyembuhan Luka Bakar. JSFK Jurnal Sains Farmasi Klinis. 2018;5(2):83-7. doi:10.25077/jsfk.5.2.83-87.2018
19. Sarlina S, Razak AR, Tandah MR. Uji Aktivitas Antibakteri Sediaan Gel Ekstrak Daun Sereh (Cymbopogon nardus L. Rendle) terhadap Bakteri Staphylococcus aureus Penyebab Jerawat. Jurnal Farmasi Galenika Galenika J Pharm. 2017;3(2):143-9. doi:10.22487/j24428744.0.v0.i0.8770
20. Almurdani M, Jose C, Teruna HY. Uji Aktivitas Antioksidan dan Toksisitas Ekstrak Akar Tanaman Amaranthus spinosus. Indones Chemia Acta. 2013;4(1):7-11.
21. Pujimulyani D, Raharjo S, Marsono Y, Santoso U. Aktivitas Antioksidan dan Kadar Senyawa Fenolik pada Kunir Putih (Curcuma mangga Val.) Segar dan Setelah Blanching. Agritech. 2010;30(2):68-74. doi:10.22146/agritech.9675
22. Jantan I, Saputri FC, Qaisar MN, Buang F. Correlation between Chemical Composition of Curcuma domestica and Curcuma xanthorrhiza and Their Antioxidant Effect on Human Low-Density Lipoprotein Oxidation. Evid Based Complement Alternat Med. 2012;2012:438356. doi:10.1155/2012/438356
23. Maharani BC, Lindriati T, Diniyah N. Pengaruh Variasi Waktu Blanching dan Konsentrasi Asam Sitrat Terhadap Karakteristik dan Aktivitas Ekstrak Pigmen Ubi Jalar Ungu (Ipomoea batatas L.). JP2 Jurnal Penelitian Pangan Indones J Food Res. 2016;1(1):60-7.
24. Hasrawati A, Hardianti H, Qama A, Wais M. Pengembangan Ekstrak Etanol Limbah Biji Pepaya (Carica papaya L.) Sebagai Serum Antijerawat. Jurnal Fitofarmaka Indonesia. 2020;7(1):1-8. doi:10.33096/jffi.v7i1.458
25. Yumas M. Formulasi Sediaan Krim Wajah Berbahan Aktif Ekstra Metanol Biji Kakao Non Fermentasi (Theobroma cacao L) Kombinasi Madu Lebah. Jurnal Industri Hasil Perkebunan. 2016;11(2):75-87. doi:10.33104/jihp.v11i2.3414
26. Directorate General of Drug and Food Control, Ministry of Health, Republic of Indonesia. Formularium Kosmetika Indonesia. Jakarta, Indonesia: Ministry of Health, Republic of Indonesia; 1985.
27. Badan Standarisasi Nasional. Sediaan Tabir Surya. SNI 16-4399-1996. Jakarta, Indonesia: Badan Standarisasi Nasional; 1996.
28. Ardana M, Aeyni V, Ibrahim A. Formulasi dan Optimasi Basis Gel HPMC (Hidroxy Propyl Methyl Cellulose) dengan Berbagai Variasi Konsentrasi. J Trop Pharm Chem. 2015;3(2):101-8. doi:10.25026/jtpc.v3i2.95
29. Tambunan S, Sulaiman TNS. Gel Formulation of Lemongrass Essential Oil with HPMC and Carbopol Bases. Majalah Farmaseutik. 2018;14(2):87-95. doi:10.22146/farmaseutik.v14i2.42598
30. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011;48(4):412-22. doi:10.1007/s13197-011-0251-1
31. Pratiwi D, Wardaniati I. Pengaruh Variasi Perlakuan (Segar dan Simplisia) Rimpang Kunyit (Curcuma domestica) terhadap Aktivitas Antioksidan dan Kadar Fenol Total. Jurnal Farmasi Higea. 2019;11(2):159-65.
Authors
1.
Pratiwi D, Sidoretno WM, Aisah N. The Combination of Turmeric (Curcuma domestica) Rhizome Extract and Collagen in A Serum Formulation as an Antioxidant. Borneo J Pharm [Internet]. 2021Feb.28 [cited 2025Apr.16];4(1):36-42. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/1578
Copyright (c) 2021 Denia Pratiwi, Wahyu Margi Sidoretno, Nur Aisah
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