Antiasthma Activities of Moringa oleifera Lam. Leaves Extract on the Eosinophil Count and Mast Cells in BALB/c Mice
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Submitted
11 December 2020
11 December 2020
Published
30 August 2021
30 August 2021
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Abstract
Asthma is a chronic inflammation of the respiratory tract. This study aimed to prove Moringa oleifera Lam. leaves extract's effect on reducing the eosinophil count and mast cells in asthmatic mice. Twenty BALB/c mice that met the inclusion criteria were divided into four groups of standard control (K1), negative control (K2), dexamethasone (K3), and M. oleifera leaves extract (K4). On days 0 and 7, intraperitoneal ovalbumin (OVA) was sensitized. On days 14, 16, and 18, mice were re-sensitized by inhalation using 1% OVA in sterile 0.9% NaCl. On days 18 to 25, K1 and K2 groups were given Na-CMC, the K3 group was given dexamethasone 1.3 µg/day, and the K4 group was given M. oleifera leaves extract 3.9 mg/day. On the 25th day, the mice were terminated to analyze the eosinophil count and stable bronchiolar mast cells. In conclusion, M. oleifera leaves extract was proven to decrease the eosinophil count with a p-value <0.05 and could stabilize bronchiolar mast cells with a p-value <0.05.
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References
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13. Meireles D, Gomes J, Lopes L, Hinzmann M, Machado J. A review of properties, nutritional and pharmaceutical applications of Moringa oleifera: integrative approach on conventional and traditional Asian medicine. Adv Tradit Med. 2020;20:495-515. doi:10.1007/s13596-020-00468-0
14. Bhattacharya A, Tiwari P, Sahu PK, Kumar S. A Review of the Phytochemical and Pharmacological Characteristics of Moringa oleifera. J Pharm Bioallied Sci. 2018;10(4):181-91. doi:10.4103/JPBS.JPBS_126_18
15. Hagiwara A, Hidaka M, Takeda S, Yoshida H, Kai H, Sugita C, et al. Anti-Allergic Action of Aqueous Extract of Moringa oleifera Lam. Leaves in Mice. Eur Med Plants. 2016;16(3):1-10. doi:10.9734/EJMP/2016/28566
16. Bush A. Pathophysiological Mechanisms of Asthma. Front Pediatr. 2019;7:68. doi:10.3389/fped.2019.00068
17. Agrawal B, Mehta A. Antiasthmatic activity of Moringa oleifera Lam: A clinical study. Indian J Pharmacol. 2008;40(1):28-31. doi:10.4103/0253-7613.40486
18. Goyal BR, Goyal RK, Mehta AA. Investigation into the mechanism of anti-asthmatic action of Moringa oleifera. J Diet Suppl. 2009;6(4):313-27. doi:10.3109/19390210903280199
19. Melgert BN, Postma DS, Kuipers I, Geerlings M, Luinge MA, van der Strate BWA, et al. Female mice are more susceptible to the development of allergic airway inflammation than male mice. Clin Exp Allergy. 2005;35(11):1496-503. doi:10.1111/j.1365-2222.2005.02362.x
20. Yardeni T, Eckhaus M, Morris HD, Huizing M, Hoogstraten-Miller S. Retro-orbital injections in mice. Lab Anim. 2011;40(5):155-60. doi:10.1038/laban0511-155
21. Puebla-Osorio N, Sarchio SNE, Ullrich SE, Byrne SN. Detection of Infiltrating Mast Cells Using a Modified Toluidine Blue Staining. Methods Mol Biol. 2017;1627:213-22. doi:10.1007/978-1-4939-7113-8_14
22. Choi Y, Lee Y, Park HS. Which Factors Associated With Activated Eosinophils Contribute to the Pathogenesis of Aspirin-Exacerbated Respiratory Disease? Allergy Asthma Immunol Res. 2019;11(3):320-9. doi:10.4168/aair.2019.11.3.320
23. McBrien CN, Menzies-Gow A. The Biology of Eosinophils and Their Role in Asthma. Front Med. 2017;4:93. doi:10.3389/fmed.2017.00093
24. Palupi DA, Martati E. Efek Pemberian Minyak Biji Kelor (Moringa Oliefera L.) Sebagai Terapi Asma Terhadap Gambaran Hispatologi Epitel Bronkiolus Mencit BALB/c. Cendekia J Pharm. 2019;3(1):37-64. doi:10.31596/cjp.v3i1.42
25. Wulandari MA, Solikhah LI, Wulan SN. Uji Toksisitas Subkronis Serbuk, Ekstrak Air, dan Ekstrak Pekat Suplemen Kalsium Daun Kelor (Moringa oleifera Lam.) pada Fungsi Hepar dan Ginjal Tikus Wistar (Rattus norvegicus). Jurnal Pangan dan Agroindustri. 2017;5(4):71-82.
26. Mwamatope B, Tembo D, Chikowe I, Kampira E, Nyirenda C. Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants. Sci Afr. 2020;9:e00481. doi:10.1016/j.sciaf.2020.e00481
27. Castillo JR, Peters SP, Busse WW. Asthma Exacerbations: Pathogenesis, Prevention, and Treatment. J Allergy Clin Immunol Pract. 2017;5(4):918-27. doi:10.1016/j.jaip.2017.05.001
28. Palupi DA, Sutanto H, Sadhana U, Dharmana E, Mahati E, Sasmito E, et al. The Effect of Nigella Sativa Oil on Number Eosinophils, Neutrophils, Serum IgE Levels to Asthma Mice Resistant of Corticosteroid. Int J Pharm Res. 2020;12(1):896-901. doi:10.31838/ijpr/2020.12.01.171
29. Méndez-Enríquez E, Hallgren J. Mast Cells and Their Progenitors in Allergic Asthma. Front Immunol. 2019;10:821. doi:10.3389/fimmu.2019.00821
30. Krystel-Whittemore M, Dileepan KN, Wood JG. Mast Cell: A Multi-Functional Master Cell. Front Immunol. 2016;6:620. doi:10.3389/fimmu.2015.00620
31. Suresh S, Chhipa AS, Gupta M, Lalotra S, Sisodia SS, Baksi R, et al. Phytochemical analysis and pharmacological evaluation of methanolic leaf extract of Moringa oleifera Lam. in ovalbumin induced allergic asthma. S Afr J Bot. 2020;130:484-93. doi:10.1016/j.sajb.2020.01.046
32. Abd Rani NZ, Kumolosasi E, Jasamai M, Jamal JA, Lam KW, Husain K. In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds. BMC Complement Altern Med. 2019;19(1):361. doi:10.1186/s12906-019-2776-1
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2. Fahy JV. Type 2 inflammation in asthma--present in most, absent in many. Nat Rev Immunol. 2015;15(1):57-65. doi:10.1038/nri3786
3. Kalolella AB. Sleeping position and reported night-time asthma symptoms and medication. Pan Afr Med J. 2016;24:59. doi:10.11604/pamj.2016.24.59.9159
4. Kaufman G. Asthma: pathophysiology, diagnosis and management. Nurs Stand. 2011;26(5):48-56. doi:10.7748/ns2011.10.26.5.48.c8744
5. Jackson DJ, Humbert M, Hirsch I, Newbold P, Gil EG. Ability of Serum IgE Concentration to Predict Exacerbation Risk and Benralizumab Efficacy for Patients with Severe Eosinophilic Asthma. Adv Ther. 2020;37(2):718-29. doi:10.1007/s12325-019-01191-2
6. Nakagome K, Nagata M. Involvement and Possible Role of Eosinophils in Asthma Exacerbation. Front Immunol. 2018;9:2220. doi:10.3389/fimmu.2018.02220
7. Tshabalala T, Ncube B, Madala NE, Nyakudya TT, Moyo HP, Sibanda M, et al. Scribbling the Cat: A Case of the “Miracle” Plant, Moringa oleifera. Plants. 2019;8(11):510. doi:10.3390/plants8110510
8. Xu YB, Chen GL, Guo MQ. Antioxidant and Anti-Inflammatory Activities of the Crude Extracts of Moringa oleifera from Kenya and Their Correlations with Flavonoids. Antioxidants. 2019;8(8):296. doi:10.3390/antiox8080296
9. Vergara-Jimenez M, Almatrafi MM, Fernandez ML. Bioactive Components in Moringa Oleifera Leaves Protect against Chronic Disease. Antioxidants. 2017;6(4):91. doi:10.3390/antiox6040091
10. Martínez-González CL, Martínez L, Martínez-Ortiz EJ, González-Trujano ME, Déciga-Campos M, Ventura-Martínez R, et al. Moringa oleifera, a species with potential analgesic and anti-inflammatory activities. Biomed Pharmacother. 2017;87:482-8. doi:10.1016/j.biopha.2016.12.107
11. Adelapo AA, Falayi OO, Oyagbemi AA. Evaluation of the analgesic, anti-inflammatory, anti-oxidant, phytochemical and toxicological properties of the methanolic leaf extract of commercially processed Moringa oleifera in some laboratory animals. J Basic Clin Physiol Pharmacol. 2015;26(5):491-9. doi:10.1515/jbcpp-2014-0105
12. Razis AFA, Ibrahim MD, Kntayya SB. Health benefits of Moringa oleifera. Asian Pac J Cancer Prev. 2014;15(20):8571-6. doi:10.7314/apjcp.2014.15.20.8571
13. Meireles D, Gomes J, Lopes L, Hinzmann M, Machado J. A review of properties, nutritional and pharmaceutical applications of Moringa oleifera: integrative approach on conventional and traditional Asian medicine. Adv Tradit Med. 2020;20:495-515. doi:10.1007/s13596-020-00468-0
14. Bhattacharya A, Tiwari P, Sahu PK, Kumar S. A Review of the Phytochemical and Pharmacological Characteristics of Moringa oleifera. J Pharm Bioallied Sci. 2018;10(4):181-91. doi:10.4103/JPBS.JPBS_126_18
15. Hagiwara A, Hidaka M, Takeda S, Yoshida H, Kai H, Sugita C, et al. Anti-Allergic Action of Aqueous Extract of Moringa oleifera Lam. Leaves in Mice. Eur Med Plants. 2016;16(3):1-10. doi:10.9734/EJMP/2016/28566
16. Bush A. Pathophysiological Mechanisms of Asthma. Front Pediatr. 2019;7:68. doi:10.3389/fped.2019.00068
17. Agrawal B, Mehta A. Antiasthmatic activity of Moringa oleifera Lam: A clinical study. Indian J Pharmacol. 2008;40(1):28-31. doi:10.4103/0253-7613.40486
18. Goyal BR, Goyal RK, Mehta AA. Investigation into the mechanism of anti-asthmatic action of Moringa oleifera. J Diet Suppl. 2009;6(4):313-27. doi:10.3109/19390210903280199
19. Melgert BN, Postma DS, Kuipers I, Geerlings M, Luinge MA, van der Strate BWA, et al. Female mice are more susceptible to the development of allergic airway inflammation than male mice. Clin Exp Allergy. 2005;35(11):1496-503. doi:10.1111/j.1365-2222.2005.02362.x
20. Yardeni T, Eckhaus M, Morris HD, Huizing M, Hoogstraten-Miller S. Retro-orbital injections in mice. Lab Anim. 2011;40(5):155-60. doi:10.1038/laban0511-155
21. Puebla-Osorio N, Sarchio SNE, Ullrich SE, Byrne SN. Detection of Infiltrating Mast Cells Using a Modified Toluidine Blue Staining. Methods Mol Biol. 2017;1627:213-22. doi:10.1007/978-1-4939-7113-8_14
22. Choi Y, Lee Y, Park HS. Which Factors Associated With Activated Eosinophils Contribute to the Pathogenesis of Aspirin-Exacerbated Respiratory Disease? Allergy Asthma Immunol Res. 2019;11(3):320-9. doi:10.4168/aair.2019.11.3.320
23. McBrien CN, Menzies-Gow A. The Biology of Eosinophils and Their Role in Asthma. Front Med. 2017;4:93. doi:10.3389/fmed.2017.00093
24. Palupi DA, Martati E. Efek Pemberian Minyak Biji Kelor (Moringa Oliefera L.) Sebagai Terapi Asma Terhadap Gambaran Hispatologi Epitel Bronkiolus Mencit BALB/c. Cendekia J Pharm. 2019;3(1):37-64. doi:10.31596/cjp.v3i1.42
25. Wulandari MA, Solikhah LI, Wulan SN. Uji Toksisitas Subkronis Serbuk, Ekstrak Air, dan Ekstrak Pekat Suplemen Kalsium Daun Kelor (Moringa oleifera Lam.) pada Fungsi Hepar dan Ginjal Tikus Wistar (Rattus norvegicus). Jurnal Pangan dan Agroindustri. 2017;5(4):71-82.
26. Mwamatope B, Tembo D, Chikowe I, Kampira E, Nyirenda C. Total phenolic contents and antioxidant activity of Senna singueana, Melia azedarach, Moringa oleifera and Lannea discolor herbal plants. Sci Afr. 2020;9:e00481. doi:10.1016/j.sciaf.2020.e00481
27. Castillo JR, Peters SP, Busse WW. Asthma Exacerbations: Pathogenesis, Prevention, and Treatment. J Allergy Clin Immunol Pract. 2017;5(4):918-27. doi:10.1016/j.jaip.2017.05.001
28. Palupi DA, Sutanto H, Sadhana U, Dharmana E, Mahati E, Sasmito E, et al. The Effect of Nigella Sativa Oil on Number Eosinophils, Neutrophils, Serum IgE Levels to Asthma Mice Resistant of Corticosteroid. Int J Pharm Res. 2020;12(1):896-901. doi:10.31838/ijpr/2020.12.01.171
29. Méndez-Enríquez E, Hallgren J. Mast Cells and Their Progenitors in Allergic Asthma. Front Immunol. 2019;10:821. doi:10.3389/fimmu.2019.00821
30. Krystel-Whittemore M, Dileepan KN, Wood JG. Mast Cell: A Multi-Functional Master Cell. Front Immunol. 2016;6:620. doi:10.3389/fimmu.2015.00620
31. Suresh S, Chhipa AS, Gupta M, Lalotra S, Sisodia SS, Baksi R, et al. Phytochemical analysis and pharmacological evaluation of methanolic leaf extract of Moringa oleifera Lam. in ovalbumin induced allergic asthma. S Afr J Bot. 2020;130:484-93. doi:10.1016/j.sajb.2020.01.046
32. Abd Rani NZ, Kumolosasi E, Jasamai M, Jamal JA, Lam KW, Husain K. In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds. BMC Complement Altern Med. 2019;19(1):361. doi:10.1186/s12906-019-2776-1
Authors
1.
Palupi DA, Prasetyowati TW, Murtiningsih D, Mahdiyah D. Antiasthma Activities of Moringa oleifera Lam. Leaves Extract on the Eosinophil Count and Mast Cells in BALB/c Mice. Borneo J Pharm [Internet]. 2021Aug.30 [cited 2025Apr.4];4(3):171-7. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/1916
Copyright (c) 2021 Dian Arsanti Palupi, Tri Wahyuni Prasetyowati, Dwi Murtiningsih, Dede Mahdiyah
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