Anti-Inflammatory and Analgesic Activity of Musa balbisiana Peels In Vivo
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Submitted
21 January 2022
Published
31 May 2022
Keywords:
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Anti-Inflammatory, Analgesic, Musa balbisiana peels
Abstract
Musa balbisiana Peels (MBP) contains high levels of flavonoids, alkaloids, tannins, saponins, and triterpenoids. Flavonoids function to slow down the inflammatory process by inhibiting the arachidonic acid, forming prostaglandins, and releasing histamine. This study aimed to examine the anti-inflammatory and analgesic effects of MBP decoction. This study used the Winter method for anti-inflammatory assay by induction of carrageenan on the soles of rat's feet and Sigmund's method for analgesic assay with intraperitoneal induction of acetic acid in mice. Group I as a negative control, group II as a positive control with diclofenac sodium, group III as a low dose (200 mg/kg BW of MBP), group IV as a medium dose (400 mg/kg BW of MBP), and group V as a high dose (800 mg/kg BW of MBP decoction). The percentage of inhibition in the anti-inflammatory test in rats for groups II, III, IV, and V was 34.43%, 17.68%, 25.53%, and 25.4%, and the percentage of effectiveness for the anti-inflammatory test, respectively, was 51.35%, 74.15%, and 74.01%. The results of the percentage inhibition of the analgesic test in mice for groups II, III, IV, and V were 55.25%, 38.52%, 44.53%, and 49.31%, and the percentage of effectiveness for the analgesic test, respectively, followed by 69.71%, 80.59%, and 89.24%. Based on the results, it can be concluded that the decoction of the MBP has an anti-inflammatory and analgesic effect.
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References
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34. Jijith US, Jayakumari S. An Apparatus for the determination of rat paw Edema during In vivo Evaluation of Anti-inflammatory agents. Res J Pharm Technol. 2020;13(5):2373-5. doi:10.5958/0974-360X.2020.00426.6
35. Sukmawati S, Yuliet Y, Hardani R. Uji Aktivitas Antiinflamasi Ekstrak Etanol Daun Pisang Ambon (Musa paradisiaca L.) terhadap Tikus Putih (Rattus norvegicus L.) yang Diinduksi Karagenan. J Farmasi Galenika Galenika J Pharm. 2015;1(2):126-32. doi:10.22487/j24428744.2015.v1.i2.6244
36. Ayertey F, Ofori-Attah E, Antwi S, Amoa-Bosompem M, Djameh G, Lartey NL, et al. Anti-inflammatory activity and mechanism of action of ethanolic leaf extract of Morinda lucida Benth. J Tradit Complement Med. 2020;11(3):249-58. doi:10.1016/j.jtcme.2020.07.001
37. Gan TJ. Diclofenac: an update on its mechanism of action and safety profile. Curr Med Res Opin. 2010;26(7):1715-31. doi:10.1185/03007995.2010.486301
38. de la Puente B, Romero-Alejo E, Vela JM, Merlos M, Zamanillo D, Portillo-Salido E. Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice. J Pain Res. 2015;8:663. doi:10.2147/jpr.s91230
39. Lavin DN, Joesting JJ, Chiu GS, Moon ML, Meng J, Dilger RN, et al. Fasting induces an anti-inflammatory effect on the neuroimmune system which a high-fat diet prevents. Obesity. 2011;19(8):1586-94. doi:10.1038/oby.2011.73
40. Faujdar S, Sharma S, Sati B, Pathak AK, Paliwal SK. Comparative analysis of analgesic and anti-inflammatory activity of bark and leaves of Acacia ferruginea DC. Beni-Suef Univ J Basic Appl Sci. 2016;5(1):70-8. doi:10.1016/j.bjbas.2016.02.002
41. Varalakshmi T, Hemalatha M, Sridevi K, Krishnan SA, Manivasagam GA. In Vitro Evaluation of Anti-Oxidant and Anti-Inflammatory Activity by using Ethanol Extract of Banana Peel. J Emerg Technol Innov Res. 2020;7(10):1671-8.
42. Bellik Y, Boukraâ L, Alzahrani HA, Bakhotmah BA, Abdellah F, Hammoudi SM, et al. Molecular mechanism underlying anti-inflammatory and anti-allergic activities of phytochemicals: an update. Molecules. 2012;18(1):322-53. doi:10.3390/molecules18010322
2. Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M, Taufiq-Yap YH, Hezmee MNM. The crucial roles of inflammatory mediators in inflammation: A review. Vet World. 2018;11(5):627-35. doi:10.14202/vetworld.2018.627-635
3. Sproston NR, Ashworth JJ. Role of C-Reactive Protein at Sites of Inflammation and Infection. Front Immunol. 2018;9:754. doi:10.3389/fimmu.2018.00754
4. Woolf CJ. What is this thing called pain? J Clin Invest. 2010;120(11):3742-4. doi:10.1172/jci45178
5. Sugimoto MA, Sousa LP, Pinho V, Perretti M, Teixeira MM. Resolution of Inflammation: What Controls Its Onset? Front Immunol. 2016;7:160. doi:10.3389/fimmu.2016.00160
6. Raja SN, Carr DB, Cohen M, Finnerup NB, Flor H, Gibson S, et al. The revised International Association for the Study of Pain definition of pain: concepts, challenges, and compromises. Pain. 2020;161(9):1976-82. doi:10.1097/j.pain.0000000000001939
7. Siler S, Borneman T, Ferrell B. Pain and Suffering. Semin Oncol Nurs. 2019;35(3):310-4. doi:10.1016/j.soncn.2019.04.013
8. Wongrakpanich S, Wongrakpanich A, Melhado K, Rangaswami J. A Comprehensive Review of Non-Steroidal Anti-Inflammatory Drug Use in The Elderly. Aging Dis. 2018;9(1):143-50. doi:10.14336/ad.2017.0306
9. Gunaydin C, Bilge SS. Effects of Nonsteroidal Anti-Inflammatory Drugs at the Molecular Level. Eurasian J Med. 2018;50(2):116-21. doi:10.5152/eurasianjmed.2018.0010
10. Bindu S, Mazumder S, Bandyopadhyay U. Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: A current perspective. Biochem Pharmacol. 2020;180:114147. doi:10.1016/j.bcp.2020.114147
11. Yuan H, Ma Q, Ye L, Piao G. The Traditional Medicine and Modern Medicine from Natural Products.Molecules. 2016;21(5):559. doi:10.3390/molecules21050559
12. Sofowora A, Ogunbodede E, Onayade A. The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med. 2013;10(5):210-29. doi:10.4314/ajtcam.v10i5.2
13. Swargiary A, Boro H, Roy MK, Akram M. Phytochemistry and Pharmacological Property of Musa balbisiana Colla: A Mini-Review. Pharmacogn Rev. 2021;15(29):91-5. doi:10.5530/phrev.2021.15.11
14. Lumowa SVT, Bardin S. Uji Fitokimia Kulit Pisang Kepok (Musa paradisiaca L.) Bahan Alam Sebagai Pestisida Nabati Berpotensi Menekan Serangan Serangga Hama Tanaman Umur Pendek. J Sains Kesehatan. 2018;1(9):465-9. doi:10.25026/jsk.v1i9.87
15. Daimari M, Swargiary A. Study of Phytochemical Content and Antioxidant Properties of Musa Balbisiana Corm Extract. Indian J Pharm Sci. 2020;82(4):707-12. doi:10.36468/pharmaceutical-sciences.698
16. Phuaklee P, Ruangnoo S, Itharat A. Anti-inflammatory and antioxidant activities of extracts from Musa sapientum peel. J Med Assoc Thai. 2012;95(Suppl 1):S142-6.
17. Panchee AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016;5:e47. doi:10.1017/jns.2016.41
18. Yahfoufi N, Alsadi N, Jambi M, Matar C. The Immunomodulatory and Anti-Inflammatory Role of Polyphenols. Nutrients. 2018;10(11):1618. doi:10.3390/nu10111618
19. Nunes CDR, Arantes MB, Pereira SMdF, da Cruz LL, Passos MdS, de Moraes LP, et al. Plants as Sources of Anti-Inflammatory Agents. Molecules. 2020;25(16):3726. doi:10.3390/molecules25163726
20. Sharifi-Rad M, Kumar NAV, Zucca P, Varoni EM, Dini L, Panzarini E, et al. Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases. Front Physiol. 2020;11:694. doi:10.3389/fphys.2020.00694
21. Carbone L, Austin J. Pain and Laboratory Animals: Publication Practices for Better Data Reproducibility and Better Animal Welfare. PLoS One. 2016;11(5):e0155001. doi:10.1371/journal.pone.0155001
22. Ganguly A, Al Mahmud Z, Uddin MMN, Rahman SMA. In-vivo anti-inflammatory and anti-pyretic activities of Manilkara zapota leaves in albino Wistar rats. Asian Pac J Trop Dis. 2013;3(4):301-7. doi:10.1016/S2222-1808(13)60073-0
23. Gawade SP. Acetic acid induced painful endogenous infliction in writhing test on mice. J Pharmacol Pharmacother. 2012;3(4):348. doi:10.4103/0976-500x.103699
24. McHugh ML. Multiple comparison analysis testing in ANOVA. Biochem Med. 2011;21(3):203-9. doi:10.11613/bm.2011.029
25. Pereira A, Maraschin M. Banana (Musa spp) from peel to pulp: Ethnopharmacology, source of bioactive compounds and its relevance for human health. J Ethnopharmacol. 2015;160:149-63. doi:10.1016/j.jep.2014.11.008
26. Li L, Wang Y, Liu F, Xu Y, Bao H. Study on the Effect of Deep Eutectic Solvent Liquid Phase Microextraction on Quality Standard, Antitussive, and Expectorant of Sangbaipi Decoction. J Anal Methods Chem. 2021;2021:9999406. doi:10.1155/2021/9999406
27. Abubakar AR, Haque M. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. J Pharm Bioallied Sci. 2020;12(1):1-10. doi:10.4103/jpbs.jpbs_175_19
28. De Luna SLR, Ramírez-Garza RE, Saldivar SOS. Environmentally Friendly Methods for Flavonoid Extraction from Plant Material: Impact of Their Operating Conditions on Yield and Antioxidant Properties. Sci World J. 2020;2020:6792069. doi:10.1155/2020/6792069
29. Yuei LP, Singaram N, Hassan H. Study of Anti-inflammatory and Analgesic Activity of Musa spp. Peel. ResearchGate: Preprint. 2016;46-53. doi:10.13140/RG.2.2.33612.10884
30. Deuis JR, Dvorakova LS, Vetter I. Methods Used to Evaluate Pain Behaviors in Rodents. Front Mol Neurosci. 2017;10:284. doi:10.3389/fnmol.2017.00284
31. Regmi B, Shah MK. Possible implications of animal models for the assessment of visceral pain. Animal Model Exp Med. 2020;3(3):215-28. doi:10.1002/ame2.12130
32. Fehrenbacher JC, Vasko MR, Duarte DB. Models of inflammation: Carrageenan- or complete Freund's Adjuvant (CFA)-induced edema and hypersensitivity in the rat. Curr Protoc Pharmacol. 2012;5:5.4. doi:10.1002/0471141755.ph0504s56
33. Patil KR, Mahajan UB, Unger BS, Goyal SN, Belemkar S, Surana SJ, et al. Animal Models of Inflammation for Screening of Anti-inflammatory Drugs: Implications for the Discovery and Development of Phytopharmaceuticals. Int J Mol Sci. 2019;20(18):4367. doi:10.3390/ijms20184367
34. Jijith US, Jayakumari S. An Apparatus for the determination of rat paw Edema during In vivo Evaluation of Anti-inflammatory agents. Res J Pharm Technol. 2020;13(5):2373-5. doi:10.5958/0974-360X.2020.00426.6
35. Sukmawati S, Yuliet Y, Hardani R. Uji Aktivitas Antiinflamasi Ekstrak Etanol Daun Pisang Ambon (Musa paradisiaca L.) terhadap Tikus Putih (Rattus norvegicus L.) yang Diinduksi Karagenan. J Farmasi Galenika Galenika J Pharm. 2015;1(2):126-32. doi:10.22487/j24428744.2015.v1.i2.6244
36. Ayertey F, Ofori-Attah E, Antwi S, Amoa-Bosompem M, Djameh G, Lartey NL, et al. Anti-inflammatory activity and mechanism of action of ethanolic leaf extract of Morinda lucida Benth. J Tradit Complement Med. 2020;11(3):249-58. doi:10.1016/j.jtcme.2020.07.001
37. Gan TJ. Diclofenac: an update on its mechanism of action and safety profile. Curr Med Res Opin. 2010;26(7):1715-31. doi:10.1185/03007995.2010.486301
38. de la Puente B, Romero-Alejo E, Vela JM, Merlos M, Zamanillo D, Portillo-Salido E. Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice. J Pain Res. 2015;8:663. doi:10.2147/jpr.s91230
39. Lavin DN, Joesting JJ, Chiu GS, Moon ML, Meng J, Dilger RN, et al. Fasting induces an anti-inflammatory effect on the neuroimmune system which a high-fat diet prevents. Obesity. 2011;19(8):1586-94. doi:10.1038/oby.2011.73
40. Faujdar S, Sharma S, Sati B, Pathak AK, Paliwal SK. Comparative analysis of analgesic and anti-inflammatory activity of bark and leaves of Acacia ferruginea DC. Beni-Suef Univ J Basic Appl Sci. 2016;5(1):70-8. doi:10.1016/j.bjbas.2016.02.002
41. Varalakshmi T, Hemalatha M, Sridevi K, Krishnan SA, Manivasagam GA. In Vitro Evaluation of Anti-Oxidant and Anti-Inflammatory Activity by using Ethanol Extract of Banana Peel. J Emerg Technol Innov Res. 2020;7(10):1671-8.
42. Bellik Y, Boukraâ L, Alzahrani HA, Bakhotmah BA, Abdellah F, Hammoudi SM, et al. Molecular mechanism underlying anti-inflammatory and anti-allergic activities of phytochemicals: an update. Molecules. 2012;18(1):322-53. doi:10.3390/molecules18010322
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1.
Sandhiutami NMD, Khairani S, Dewi RS, Hakim ZR, Pradani AR. Anti-Inflammatory and Analgesic Activity of Musa balbisiana Peels In Vivo. Borneo J Pharm [Internet]. 2022May31 [cited 2024Nov.17];5(2):81-92. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/3169
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