Tyrosinase Inhibition Activity and Phytochemical Screening of Melaleuca leucadendron L. Leaves
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Phytochemical screening, Tyrosinase, Melaleuca leucadendron L.
Abstract
Melaleuca leucadendron L. is a plant whose almost all parts (bark, leaves, twigs, and fruit) can be used as medicine, such as antioxidants, antifungals, sedative effects, and anti-hyaluronidase. This research was conducted to determine tyrosinase inhibition activity and compound content of M. leucadendron leaves. Maceration of M. leucadendron leaves was done in methanol, then carried out liquid-liquid fractionation with n-butanol, chloroform, and water. Methanol extract, butanol fraction, chloroform fraction, and water fraction were tested for phytochemical screening and tyrosinase inhibition using L-DOPA substrate with an ELISA plate well reader. The results of the tyrosinase inhibition activity test at concentrations of 100, 1000 and 10000 μg/mL respectively showed that methanol extract 29.532%, 55.227%, 89.583%; butanol fraction 29.313%, 59.174%, 94.737%, chloroform fraction 21.820%, 24.671%; 53.765%; water fraction 24,086%, 47.661%, 91.118%. Inhibition of the tyrosinase enzyme is shown through the IC50 value from methanol extract, butanol fraction and water fraction, and kojic acid as a positive control, respectively 645.438 μg/mL, 517.935 μg/mL, 669.403 μg/mL, 50.064 μg/mL. Phytochemical screening showed that the extract and fraction contained tannins, flavonoids, saponins, terpenes, and steroids. These results indicate that the butanol fraction is more potent as an anti-tyrosinase agent than the others.
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References
2. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017;2017:8416763. doi:10.1155/2017/8416763
3. Upadhyay PR, Starner RJ, Swope VB, Wakamatsu K, Ito S, Abdel-Malek ZA. Differential Induction of Reactive Oxygen Species and Expression of Antioxidant Enzymes in Human Melanocytes Correlate with Melanin Content: Implications on the Response to Solar UV and Melanoma Susceptibility. Antioxidants. 2022;11(6):1204. doi:10.3390/antiox11061204
4. Xing X, Dan Y, Xu Z, Xiang L. Implications of Oxidative Stress in the Pathogenesis and Treatment of Hyperpigmentation Disorders. Oxd Med Cell Longev. 2022;2022:7881717. doi:10.1155/2022/7881717
5. Desai SR. Hyperpigmentation therapy: a review. J Clin Aesthet Dermatol. 2014;7(8):13-7.
6. da Silva AP, Silva NdF, Andrade EHA, Gratieri T, Setzer WN, Maia JGS, et al. Tyrosinase inhibitory activity, molecular docking studies and antioxidant potential of chemotypes of Lippia origanoides (Verbenaceae) essential oils. PLoS One. 2017;12(5):e0175598. doi:10.1371/journal.pone.0175598
7. Pillaiyar T, Manickam M, Namasivayam V. Skin whitening agents: medicinal chemistry perspective of tyrosinase inhibitors. J Enzyme Inhib Med Chem. 2017;32(1):403-25. doi:10.1080/14756366.2016.1256882
8. Ito S, Wakamatsu K. Chemistry of mixed melanogenesis--pivotal roles of dopaquinone. Photochem Photobiol. 2008;84(3):582-92. doi:10.1111/j.1751-1097.2007.00238.x
9. Zolghadri S, Bahrami A, Khan MTH, Munoz-Munoz J, Garcia-Molina F, Garcia-Canovas F, et al. A comprehensive review on tyrosinase inhibitors. J Enzyme Inhib Med Chem. 2019;34(1):279-309. doi:10.1080/14756366.2018.1545767
10. David S, Hamilton JP. Drug-induced Liver Injury. US Gastroenterol Hepatol Rev. 2010;6:73-80.
11. Boo YC. Arbutin as a Skin Depigmenting Agent with Antimelanogenic and Antioxidant Properties. Antioxidants. 2021;10(7):1129. doi:10.3390/antiox10071129
12. Owolabi JO, Fabiyi OS, Adelakin LA, Ekwerike MC. Effects of Skin Lightening Cream Agents - Hydroquinone and Kojic Acid, on the Skin of Adult Female Experimental Rats. Clin Cosmet Investig Dermatol. 2020;13:283-9. doi:10.2147/ccid.s233185
13. Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016;5:e47. doi:10.1017/jns.2016.41
14. El-Nashar HAS, El-Din MIG, Hritcu L, Eldahshan OA. Insights on the Inhibitory Power of Flavonoids on Tyrosinase Activity: A Survey from 2016 to 2021. Molecules. 2021;26(24):7546. doi:10.3390/molecules26247546
15. Obaid RJ, Mughal U, Naeem N, Sadiq A, Alsantali RI, Jassas RS, et al. Natural and synthetic flavonoid derivatives as new potential tyrosinase inhibitors : a systematic review. RSC Advances. 2021;11:22159–98. doi:10.1039/D1RA03196A
16. Ashraf Z, Rafiq M, Nadeem H, Hassan M, Afzal S, Waseem M, et al. Carvacrol derivatives as mushroom tyrosinase inhibitors; synthesis, kinetics mechanism and molecular docking studies. PLoS One. 2017;12(5):e0178069. doi:10.1371/journal.pone.0178069
17. Wu B, Zhang X, Wu X. New lignan glucosides with tyrosinase inhibitory activities from exocarp of Castanea henryi. Carbohydr Res. 2012;355:45–9. doi:10.1016/j.carres.2012.04.009
18. Surh J, Yun JM. Antioxidant and anti-inflammatory activities of butanol extract of Melaleuca leucadendron L. Prev Nutr Food Sci. 2012;17(1):22–8. doi:10.3746/pnf.2012.17.1.022
19. Sembiring EN, Elya B, Sauriasari R. Phytochemical screening, total flavonoid and total phenolic content and antioxidant activity of different parts of Caesalpinia bonduc (L.) Roxb. Pharmacogn J. 2018;10(1):123–7. doi:10.5530/pj.2018.1.22
20. Arifianti AE, Anwar E, Nurjanah. Tyrosinase Inhibitor and Antioxidant Activity of Seaweed Powder from Fresh and Dried Sargassum plagyophyllum. J Pengolahan Hasil Perikanan Indones. 2017;20(3):488-93. doi:10.17844/jphpi.v20i3.19769
21. Uddin MS, Ferdosh S, Akanda MJH, Ghafoor K, Rukshana AH, Ali ME, et al. Techniques for the extraction of phytosterols and their benefits in human health: a review. Sep Sci Technol. 2018;53(14):2206-23. doi:10.1080/01496395.2018.1454472
22. Khongsai S, Vittaya L. Solvent Effect on Phytochemical Screening of Melaleuca leucadendra Linn. and Syzygium cinerea. Rajamangala Univ Technol Srivijaya Res J. 2019;12(1):112–9.
23. Song Y, Chen S, Li L, Zeng Y, Hu X. The Hypopigmentation Mechanism of Tyrosinase Inhibitory Peptides Derived from Food Proteins: An Overview. Molecules. 2022;27(9):2710. doi:10.3390/molecules27092710
24. Phasha V, Senabe J, Ndzotoyi P, Okole B, Fouche G, Chuturgoon A. Review on the Use of Kojic Acid—A Skin-Lightening Ingredient. Cosmetics. 2022;9(3):64. doi:10.3390/cosmetics9030064
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