An Initial Investigation of the Potential of Robusta Coffee, Arabica Coffee, and Caffeine in Asthma Treatment through the Evaluation of 5-Lipoxygenase Inhibition Activity

Tegar Achsendo Yuniarta (1) , Rosita Handayani (2)
(1) University of Surabaya , Indonesia
(2) Airlangga University , Indonesia


Numerous studies have documented the potential of coffee to aid in asthma prevention. Nevertheless, research into how coffee influences asthma management has not been available. One known mechanism by which asthma medications work involves inhibiting 5-lipoxygenase (5-LOX) activity. This study aims to determine the potency of Coffea canephora var. Robusta extract (CRE), Coffea arabica extract (CAE), and caffeine are the primary isolates against 5-LOX activity. Extraction was performed by a reflux procedure using 96% ethanol with a sample-total solvent ratio of 1 : 10, an extraction time of 1 hour, and the extraction was conducted in triplicate. Fractionation was carried out by liquid-liquid partition using a chloroform-water system. Caffeine further purification was performed by the sublimation method, and the inhibition of 5-LOX activity was evaluated using the spectrophotometric method at λ = 234 nm. Apigenin was used as a positive control. From the experiment conducted, the IC50 of the CRE, CAE, caffeine, and apigenin against 5-LOX was 32.2 ± 1.4, 42.1 ± 2.3, 14.3 ± 1.6, and 7.4 ± 1.7 µg/mL, respectively. Continued efforts to isolate bioactive compounds from coffee extract led to the discovery of caffeine, which exhibited a more potent inhibitory effect on 5-LOX. The inhibition of 5-LOX activity by caffeine occurs in a non-competitive manner.

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Tegar Achsendo Yuniarta
Rosita Handayani (Primary Contact)
Author Biographies

Tegar Achsendo Yuniarta, University of Surabaya

Department of Pharmacy, Universitas Surabaya, Surabaya, East Java, Indonesia

Rosita Handayani, Airlangga University

Department of Pharmacy, Universitas Airlangga, Surabaya, East Java, Indonesia

Department of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

Yuniarta TA, Handayani R. An Initial Investigation of the Potential of Robusta Coffee, Arabica Coffee, and Caffeine in Asthma Treatment through the Evaluation of 5-Lipoxygenase Inhibition Activity. Borneo J Pharm [Internet]. 2024Feb.29 [cited 2024May28];7(1):80-8. Available from:

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