Characterization of Secondary Metabolites Profile from Methanol Fraction of Temurui (Murraya koenigii (Linn.) Spreng) Leaves Using UPLC-MS

Intania Permata (1) , Adlis Santoni (2) , Afrizal Afrizal (3) , Trisno Afandi (4)
(1) Politeknik ATI Padang , Indonesia
(2) Universitas Andalas , Indonesia
(3) Universitas Andalas , Indonesia
(4) Politeknik Teknologi Kimia Industri Medan , Indonesia


The leaves of the temurui (Murraya koenigii (Linn.) Spreng) plant have long been known and used by Indonesian and even Asian people as a traditional medicine to treat stomach aches and diabetes. This study aimed to determine the secondary metabolite profile of the methanol extract of M. koenigii leaves. Murraya koenigii leaves fine powder was extracted in stages using n-hexane, ethyl acetate, and methanol. Each extract was prepared using a mixture of water, formic acid, acetonitrile, and formic acid, then injected into the UPLC-MS, then analyzed with MassLynx and ChemSpider. The results showed that the metabolite profile of the methanol extract of M. koenigii leaves contained 13 compounds, including phenolic, steroid, and alkaloid groups. Those compounds could be tested to identify their bioactivity.

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1. Groom B, Palmer C, Sileci L. Carbon emissions reductions from Indonesia's moratorium on forest concessions are cost-effective yet contribute little to Paris pledges. Proc Natl Acad U S A. 2022;119(5):e2102613119. doi:10.1073/pnas.2102613119
2. Salmerón-Manzano E, Garrido-Cardenas JA, Manzano-Agugliaro F. Worldwide research trends on medicinal plants. Int J Environ Res Public Health. 2020;17(10):3376. doi:10.3390/ijerph17103376
3. Zaky AA, Simal-Gindara J, Eun JB, Shim JH, El-Aty AMA. Bioactivities, Applications, Safety, and Health Benefits of Bioactive Peptides From Food and By-Products: A Review. Front Nutr. 2022;8:815640. doi:10.3389/fnut.2021.815640
4. Mickymaray S. Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens. Antibiotics. 2019;8(4):257. doi:10.3390/antibiotics8040257
5. Khakurel D, Uprety Y, Ahn G, Cha YJ, Kim WY, Lee SH, et al. Diversity, distribution, and sustainability of traditional medicinal plants in Kaski district, western Nepal. Front Pharmacol. 2022;13:1076351. doi:10.3389/fphar.2022.1076351
6. Sharifi-Rad M, Mnayer D, Morais-Braga MFB, Carneiro JNP, Bezerra CF, Coutinho HDM, et al. Echinacea plants as antioxidant and antibacterial agents: From traditional medicine to biotechnological applications. Phytother Res. 2018;32(9):1653–63. doi:10.1002/ptr.6101
7. Erb M, Kliebenstein DJ. Plant Secondary Metabolites as Defenses, Regulators, and Primary Metabolites: The Blurred Functional Trichotomy. Plant Physiol. 2020;184(1):39-52. doi:10.1104/pp.20.00433
8. van Wyk AS, Prinsloo G. Medicinal plant harvesting, sustainability and cultivation in South Africa, Biol Conserv. 2018;227:335–42. doi:10.1016/j.biocon.2018.09.0185
9. Nayak A, Mandal S, Banerji A, Banerji J. Review on chemistry and pharmacology of Murraya koenigii Spreng (Rutaceae). J Chem Pharm Res. 2010;2(2):286-99.
10. Sindhu RK, Arora S. Evaluation of phenolic contents and antioxidant potential of Murraya koenigii (L) Spreng roots. J Appl Pharm Sci. 2012;2(11):120-2. doi:10.7324/JAPS.2012.21121
11. Upadhye MC, Deokate UA, Pujari RR. Antidiabetic effect of ethanolic extract of Murraya koenigii (Linn.) stem bark in alloxan induced diabetic rats. Int J Pharmacogn. 2019;6(6):193–201. doi:10.13040/IJPSR.0975-8232.IJP.6(6).193-01
12. Abeysinghe DT, Alwis DDDH, Kumara KAH, Chandrika UG. Nutritive Importance and Therapeutics Uses of Three Different Varieties ( Murraya koenigii, Micromelum minutum, and Clausena indica) of Curry Leaves: An Updated Review. Evid Based Complement Alternat Med. 2021;2021:5523252. doi:10.1155/2021/5523252
13. Fachraniah, Kurniasih E, Novilasi DT. Ekstraksi Antioksidan dari Daun Kari. J Sains Teknologi Reaksi. 2012;10:35-44.
14. Ali AI, Paul V, Chattree A, Prasad R, Paul A, Amiteye D. Evaluation of the use of different solvents for phytochemical constituents and antioxidants activity of the leaves of Murraya Koenigii (Linn.) Spreng. (rutaceae). Plant Arch. 2021;21(1):985-92. doi:10.51470/plantarchives.2021.v21.no1.137
15. Sanmugarajah V, Rajkumar G. A Review of Anti-hyperglycemic Effects of Curry Leaf Tree (Murraya koenigii). Borneo J Pharm. 2022;5(2):104-114. doi:10.33084/bjop.v5i2.3300
16. Samanta SK, Kandimalla R, Gogoi B, Dutta KN, Choudhury P, Deb PK, et al. Phytochemical portfolio and anticancer activity of Murraya koenigii and its primary active component, mahanine. Pharmacol Res. 2018;129:227–36. doi:10.1016/j.phrs.2017.11.024
17. Gaidhane AJ, Chandewar AB, Ambadkar BB, Shoeb S. Phytochemical screening and antimicrobial investigation of Murraya koenigii. World J Pharm Res. 2020;84(3):1206–20. doi:10.20959/wjpr20203-16899
18. Anshari MK, Rusdi B. Studi literatur senyawa aktif antibakteri dari ekstrak daun salam koja (Murraya koenigii (Linn) Spreng). J Riset Farmasi. 2021;1(2):156–65. doi:10.29313/jrf.v1i2.571
19. Sukma FF, Sahara D, Ihsan FN, Halimatussakdiah, Wahyuningsih P, Amna U. Skrining fitokimia ekstrak daun “temurui” (Murraya koenigii (L.) Spreng) Kota Langsa, Aceh. J Jeumpa J Pendidikan Sains Biol. 2018;5(1):34-9.
20. Malode GP, Parbat AY, Shaikh AR, Panchale WA, Manwar JV, Bakal RL. Phytochemistry, pharmacology and botanical aspects of Murraya koenigii in the search for molecules with bioactive potential - A review. GSC Adv Res Rev. 2021;6(3):143–55. doi:10.30574/gscarr.2021.6.3.005
21. Chakraborty M. Mumunine - A New Carbazole Alkaloid from Murraya koenigii (Linn.) Spreng. J Sci Res. 2020;12(4):665–72. doi:10.3329/jsr.v12i4.45499
22. Amna U, Halimatussakdiah H, Wahyuningsih P, Saidi N, Nasution R, Astryna SY. Phytochemical screening and in vitro Cytotoxic activity of Hexane extract of Temurui (Murraya koenigii (Linn.) Spreng) leaves against Human Cervical Cancer (HeLa) cell line. IOP Conf Ser Mater Sci Eng. 523:012018. doi:10.1088/1757-899X/523/1/012018
23. Arunkumar J. Evaluation of anti-inflammatory activity of methanolic extract of murraya koenigii leaves in experimental animals. Univ J Pre Para Clin Sci. 2021;7(1):17340.
24. Mathur A, Verma SK, Singh SK, Prasad GBKS, Dua VK. Investigation of the antimicrobial, antioxidant and anti-inflammatory activity of compound isolated from Murraya koenigii. Int J Appl Biol Pharm Technol. 2011;2(1):1-8.
25. Fiehn O. Metabolomics by Gas Chromatography-Mass Spectrometry: Combined Targeted and Untargeted Profiling. Cur Protoc Mol Biol. 2016;114:30.4.1-32. doi:10.1002/0471142727.mb3004s114
26. Pant DR, Pant ND, Saru DB, Yadav UN, Khanal DP. Phytochemical screening and study of antioxidant, antimicrobial, antidiabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh. J Intercult Ethnopharmacol. 2017;6(2):170-6. doi:10.5455/jice.20170403094055
27. Wutsqa YU, Sratman, Sari SLA. Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography. Asian J Nat Prod Biochem. 2021;19(2):66-9. doi:10.13057/biofar/f190204
28. Werner E, Croixmarie V, Umbdenstock T, Ezan E, Chaminade P, Tabet JC, et al. Mass spectrometry-based metabolomics: accelerating the characterization of discriminating signals by combining statistical correlations and ultrahigh resolution. Anal Chem. 2008;80(13):4918-32. doi:10.1021/ac800094p
29. Vijlder TD, Valkenborg D, Lemière F, Romjin EP, Laukens K, Cuyckens F. A tutorial in small molecule identification via electrospray ionization-mass spectrometry: The practical art of structural elucidation. Mass Spectrom Rev. 2018;37(5):607-29. doi:10.1002/mas.21551
30. Isnindar, Wahyuono S, Setyowati EP. Isolation and Identification of Antioxidant Compound of Persimmon Leaves (Diospyros kaki Thunb.) using DPPH (2,2 diphenyl-1-pikrilhidrazil) Method. Majalah Obat Tradisional. 2011;16(3):161- 9. doi:10.22146/tradmedj.8054
31. Ahlem CN, Auci DL, Nicoletti F, Pieters R, Kennedy MR, et al. Pharmacology and immune modulating properties of 5-androstene-3, 7,17-triol, a DHEA metabolite in the human metabolome. J Steroid Biochem Mol Biol. 2011;126(3-5):87-94. doi:10.1016/j.jsbmb.2011.04.010


Intania Permata
Adlis Santoni (Primary Contact)
Afrizal Afrizal
Trisno Afandi
Author Biographies

Intania Permata, Politeknik ATI Padang

Department of Chemistry, Universitas Andalas, Padang, West Sumatra, Indonesia

Department of Chemical Analysis, Politeknik ATI Padang, Padang, West Sumatra, Indonesia

Adlis Santoni, Universitas Andalas

Department of Chemistry, Universitas Andalas, Padang, West Sumatra, Indonesia

Afrizal Afrizal, Universitas Andalas

Department of Chemistry, Universitas Andalas, Padang, West Sumatra, Indonesia

Trisno Afandi, Politeknik Teknologi Kimia Industri Medan

Department of Chemical Engineering, Politeknik Teknologi Kimia Industri Medan, Medan, North Sumatra, Indonesia

Permata I, Santoni A, Afrizal A, Afandi T. Characterization of Secondary Metabolites Profile from Methanol Fraction of Temurui (Murraya koenigii (Linn.) Spreng) Leaves Using UPLC-MS. Borneo J Pharm [Internet]. 2023Aug.30 [cited 2023Dec.10];6(3):278-86. Available from:

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