In Vitro Evaluation of Prebiotic Potential of Red Ginger (Zingiber officinale var. rubrum) Rhizome Ethanol Extract on Lactobacillus acidophilus and Escherichia coli

Fadil Rido Gumelar (1) , Farendina Suarantika (2) , Bertha Rusdi (3)
(1) Bandung Islamic University , Indonesia
(2) Bandung Islamic University , Indonesia
(3) Bandung Islamic University , Indonesia

Abstract

Prebiotics, including carbohydrates and phenols, promote beneficial gut bacteria (probiotics). Red ginger (Zingiber officinale var. rubrum) rhizomes, rich in these compounds, have been traditionally used in medicine but their prebiotic potential remains unexplored. This study investigated the in vitro prebiotic effects of Z. officinale var. rubrum rhizomes on Lactobacillus acidophilus (beneficial) and Escherichia coli (opportunistic) bacteria. Prebiotic activity was assessed using a turbidimetric method, measuring bacterial growth via UV-Vis spectrophotometry at 600 nm. The prebiotic index and percentage inhibition were calculated to evaluate the impact on bacterial growth. Additionally, total phenol content was determined using the Folin-Ciocalteu method. Results indicate that Z. officinale var. rubrum rhizomes exhibit prebiotic properties, stimulating L. acidophilus growth (prebiotic index of 156.035 and percentage inhibition value of -153.128%) while inhibiting E. coli growth (54.343% inhibition). The rhizomes contained 31.15 mg GAE/g extract of total phenols and 23.55% carbohydrates. These findings suggest that Z. officinale var. rubrum rhizomes possess prebiotic potential, warranting further investigation for potential applications in gut health management.

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Authors

Fadil Rido Gumelar
Farendina Suarantika
Bertha Rusdi
bertha.rusdi78@gmail.com (Primary Contact)
Author Biographies

Fadil Rido Gumelar, Bandung Islamic University

Department of Pharmacy, Universitas Islam Bandung, Bandung, West Java, Indonesia

Farendina Suarantika, Bandung Islamic University

Department of Pharmacist Profession, Universitas Islam Bandung, Bandung, West Java, Indonesia

Bertha Rusdi, Bandung Islamic University

Department of Pharmacist Profession, Universitas Islam Bandung, Bandung, West Java, Indonesia

1.
Gumelar FR, Suarantika F, Rusdi B. In Vitro Evaluation of Prebiotic Potential of Red Ginger (Zingiber officinale var. rubrum) Rhizome Ethanol Extract on Lactobacillus acidophilus and Escherichia coli. Borneo J Pharm [Internet]. 2024Aug.30 [cited 2024Dec.5];7(3):290-6. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/6029

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