Piper cubeba L. f. Nanoemulsion: Formulation and Effect on Antioxidant Activity in Rat's Hippocampus and Prefrontal Cortex
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19 November 2024
30 May 2025
Abstract
The previous study showed that Piper cubeba extract increased brain antioxidant capacity, alleviating cognitive impairment in rats. However, brain-targeted drug delivery is often limited by the blood-brain barrier. One of the approaches to overcome this obstacle is nanocarrier. This study aimed to formulate a nanoemulsion (NE) of P. cubeba extract and determine the antioxidant activity in the brain. The research begins with the determination of excipients and the formula of NE. The formula was then tested for globule size, polydispersity index, thermodynamic stability, zeta potential, pH, and viscosity. The globule's shape was determined using transmission electron microscopy (TEM). The antioxidant activity was tested by catalase and lipid peroxidase activity in the brain after giving NE orally for seven days. Formulations of NE resulted in nano-size globules and were stable thermodynamically. The NE formula significantly increased catalase and inhibited lipid peroxidase activities in the Rat's hippocampus and prefrontal cortex. This study resulted in a stable formula and low-cost production of P. cubeba extract NE, which is active as the brain's antioxidant and is feasible to develop as a neuroprotective agent for various neurodegenerative diseases.
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References
2. Almutary AG, Begum MY, Siddiqua A, Gupta S, Chauhan P, Wadhwa K, et al. Unlocking the Neuroprotective Potential of Silymarin: A Promising Ally in Safeguarding the Brain from Alzheimer’s Disease and Other Neurological Disorders. Mol Neurobiol. 2025;62(6):7975-97. DOI: 10.1007/s12035-024-04654-y; PMID: 39956886
3. Olufunmilayo EO, Gerke-Duncan MB, Holsinger RMD. Oxidative Stress and Antioxidants in Neurodegenerative Disorders. Antioxidants. 2023;12(2):517. DOI: 10.3390/antiox12020517; PMCID: PMC9952099; PMID: 36830075
4. Ashok A, Andrabi SS, Mansoor S, Kuang Y, Kwon BK, Labhasetwar V. Antioxidant Therapy in Oxidative Stress-Induced Neurodegenerative Diseases: Role of Nanoparticle-Based Drug Delivery Systems in Clinical Translation. Antioxidants. 2022;11(2):408. DOI: 10.3390/antiox11020408; PMCID: PMC8869281; PMID: 35204290
5. Mutoh T, Mutoh T, Taki Y, Ishikawa T. Therapeutic potential of natural product-based oral nanomedicines for stroke prevention. J Med Food. 2016;19(6):521–7. DOI: 10.1089/jmf.2015.3644; PMCID: PMC4904166; PMID: 27136062
6. Xu SY, Pan SY. The failure of animal models of neuroprotection in acute ischemic stroke to translate to clinical efficacy. Med Sci Monit Basic Res. 2013;19:37–45. DOI: 10.12659/MSMBR.883750; PMCID: PMC3638705; PMID: 23353570
7. Naqvi S, Panghal A, Flora SJS. Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs. Front Neurosci. 2020;14:494. DOI: 10.3389/fnins.2020.00494; PMCID: PMC7297271; PMID: 32581676
8. Dwita LP, Iwo MI, Elfahmi, Mauludin R. Brain Antioxidant Properties of Piper cubeba L. Extracts and Essential Oil. Farmacia. 2023;71(2):296–302. DOI: 10.31925/farmacia.2023.2.9
9. Azeem A, Rizwan M, Ahmad FJ, Iqbal Z, Khar RK, Aqil M, et al. Nanoemulsion components screening and selection: A technical note. AAPS PharmSciTech. 2009;10(1):69–76. DOI: 10.1208/s12249-008-9178-x; PMCID: PMC2663668; PMID: 19148761
10. Choudhury H, Fadhilah N, Zakaria B, Atdriann P, Tilang B, Tzeyung AS, et al. Formulation development and evaluation of rotigotine mucoadhesive nanoemulsion for intranasal delivery. J Drug Deliv Sci Technol. 2019;54:101301. DOI: 10.1016/j.jddst.2019.101301
11. Hadwan MH. Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC Biochem. 2018;19:7. DOI: 10.1186/s12858-018-0097-5
12. Shakeel F, Haq N, Alanazi FK, Alsarra IA. Impact of various nonionic surfactants on self-nanoemulsification efficiency of two grades of Capryol (Capryol-90 and Capryol-PGMC). J Mol Liq. 2013;182:57-63. DOI: 10.1016/j.molliq.2013.03.011
13. de Souza RF, de Moraes SRA, Augusto RL, Zanona AdF, Matos D, Aidar FJ, et al. Endurance training on rodent brain antioxidant capacity: A meta-analysis. Neurosci Res. 2019;145:1-9. DOI: 10.1016/j.neures.2018.09.002; PMID: 30326252
14. Davis CK, Vemuganti R. Antioxidant therapies in traumatic brain injury. Neurochem Int. 2022;152:105255. DOI: 10.1016/j.neuint.2021.105255; PMCID: PMC11884749; PMID: 34915062
15. Jomova K, Raptova R, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, et al. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Arch Toxicol. 2023;97(10):2499-574. DOI: 10.1007/s00204-023-03562-9; PMCID: PMC10475008; PMID: 37597078
16. Lalkovičová M, Danielisová V. Neuroprotection and antioxidants. Neural Regen Res. 2016;11(6):865–74. DOI: 10.4103/1673-5374.184447; PMCID: PMC4962567; PMID: 27482198
17. Gupta P, Kaur T, Lavisha M, Monika G. Role of inflammation and oxidative stress in chemotherapy ‑ induced neurotoxicity. Immunol Res. 2022;70(6):725-41. DOI: 10.1007/s12026-022-09307-7; PMID: 35859244
18. Rastegar-Moghaddam SH, Alipour F, Hosseini M, Ebrahimzadeh-bideskan A. Anti-apoptotic and neurogenic properties in the hippocampus as possible mechanisms for learning and memory improving impacts of vitamin D in hypothyroid rats during the growth period. Life Sci. 2023;312:121209. DOI: 10.1016/j.lfs.2022.121209; PMID: 36410409
19. Lee KH, Cha M, Lee BH. Neuroprotective Effect of Antioxidants in the Brain. Int J Mol Sci. 2020;21(19):7152. DOI: 10.3390/ijms21197152; PMCID: PMC7582347; PMID: 32998277
20. Kamat CD, Gadal S, Mhatre M, Williamson KS, Pye QN, Hensley K. Antioxidants in central nervous system diseases: preclinical promise and translational challenges. J Alzheimers Dis. 2008;15(3):473-93. DOI: 10.3233/jad-2008-15314; PMCID: PMC2669703; PMID: 18997301
21. Fachel FNS, Schuh RS, Veras KS, Bassani VL, Koester LS, Henriques AT, et al. An overview of the neuroprotective potential of rosmarinic acid and its association with nanotechnology-based delivery systems: A novel approach to treating neurodegenerative disorders. Neurochem Int. 2019;122:47–58. DOI: 10.1016/j.neuint.2018.11.003; PMID: 30439384
22. Subedi L, Gaire BP. Tanshinone IIA: A phytochemical as a promising drug candidate for neurodegenerative diseases. Pharmacol Res. 2021;169:105661. DOI: 10.1016/j.phrs.2021.105661; PMID: 33971269
23. Singh SK, Srivastav S, Castellani RJ, Plascencia-Villa G, Perry G. Neuroprotective and Antioxidant Effect of Ginkgo biloba Extract Against AD and Other Neurological Disorders. Neurotherapeutics. 2019;16(3):666–74. DOI: 10.1007/s13311-019-00767-8; PMCID: PMC6694352; PMID: 31376068
24. Nandi A, Yan L, Jana CK, Das N. Review Article Role of Catalase in Oxidative Stress- and Age-Associated Degenerative Diseases. Oxid Med Cell Longev. 2019;2019:9613090. DOI: 10.1155/2019/9613090; PMCID: PMC6885225; PMID: 31827713
25. Sigurdsson T, Duvarci S. Hippocampal-prefrontal interactions in cognition, behavior and psychiatric disease. Front Syst Neurosci. 2016;9:190. DOI: 10.3389/fnsys.2015.00190; PMCID: PMC4727104; PMID: 26858612
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Copyright (c) 2025 Lusi Putri Dwita, Maria Immaculata Iwo, Rachmat Mauludin, Elfahmi Elfahmi
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