Aktivitas Antibakteri dan Penetapan Kadar Flavonoid Fraksi Daun Kalangkala (Litsea angulata) Serta Profil Kromatografi Lapis Tipis
Antibacterial Activity and Determination of Flavonoid Levels of Kalangkala Leaf Fraction (Litsea angulata) and Thin Layer Chromatography Profile
DOI:
https://doi.org/10.33084/jsm.v9i1.5194Keywords:
Kalangkala, Antibakteri, Flavonoid, FraksiAbstract
Traditionally the people of Kalimantan use kalangkala (Litsea angulata) to treat various diseases, one of which is diarrhea. It is known that kalangkala contains flavonoids that have antibacterial activity. Kalangkala leaf extract was tested for antibacterial activity against Escherichia coli which is one of the bacteria that causes diarrhea by diffusion and dilution. Then flavonoid levels are determined by the Spectophotometry UV-Vis method and see how thin layer chromatography profiles at the fraction level to find out how much flavonoid compounds there are. The results showed that kalangkala leaf extract has antibacterial activity against E.coli by diffusion obtained the diameter of the inhibitory zone already visible at a concentration of 20 mg / ml. Dilutionally obtained the value of KHM at a concentration of 50% and KBM at a concentration of 100%. Flavonoid levels in the extract were 71,367 mg QE(Quercetin Equialen)/g, at the n-Hexane fraction of 8,367 mg QE(Quercetin Equialen)/g, at the ethyl acetate fraction of 6,700 mg QE(Quercetin Equialen)/g and at the aquadest fraction of 5,700 mg QE(Quercetin Equialen)/g. Profile of Thin-Layered Chromatogaphy of the n-Hexane fraction with n-Hexane : Ethyl Acetate (8:12) eluene appeared 7 stains, ethyl acetate fraction with ethyl acetate : N-Hexane (3:7) eluene appears 5 stains and aquadest fraction with butanol: acetic acid : aquadest (4:1:5) eluent appears 2 stains.
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Amalia Sri, Wahdaningsih Sri dan Untari Eka Kartika. 2014. Uji Aktivitas Antibakteri Fraksi n-Heksan Kulit Buah Naga Merah (Hylocereus polyrhizus Britton & Rose) terhadap Bakteri Staphylococcus aureus ATCC 25923. Trad Med: Vol. II. - hal. ISSN 1410-5918.
Asmorowati H dan Lindawati N. 2019. Penetapan Kadar Flavonoid Total Alpukat (Persea americana Mill.) dengan Metode Spektrofotometri. Jurnal Ilmiah Farmasi: Vol. II. - hal. 51–63.
Dewi I, Saptawati T dan Rachma F. 2021. Skrining Fitokimia Ekstrak Etanol Kulit dan Biji Terong Belanda (Solanum betaceum Cav .). Prosiding Seminar Nasional UNIMUS: hal. 1210–1218.
Hapsari dan Maria Endah. 2015. Uji Aktivitas Antibakteri Ekstrak Herba Meniran (Phyllanthus ninuri) terhadap Pertumbuhan Bakteri Bacillus cereus dan Escherichia coli. Yogyakarta : Universitas Sanata Dharma
Harborne J. 1987. Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan. Bandung : ITB Bandung
Illing I, Safitri W dan Erfiana. 2017. Uji Fitokimia Ekstrak Buah Dengen. Jurnal Dinamika: hal. 66-84.
Jannah R, Darsono P, V dan Rohama. 2018. SCREENING OF PHYTOCHEMICALS AND ANTIBACTERIAL ACTIVITY OF DADANGKAK ROOTS EXTRACTS (Hydrolea spinosa L.) AGAINST Streptococcus mutans. International Conference on Health and Science: Vol. I.
Kurniawati, D. 2021. Formulasi dan Uji Aktivitas Antiseptik dari Bahan Alam Kulit Jeruk Nipis, Daun Sirih dan Tanaman Bundung terhadap Staphylococcus aureus dan Candida albican. FARMASIS: Jurnal Sains Farmasi, 2(1), 25-31.
Kuspradini H. 2018. Phytochemical, antioxidant and antimicrobial properties of litsea angulata extracts. National Library of Madicine: hal. 1-11.
Kuspradini H, Putri A, S dan Diana R. 2018. Potensi Tumbuhan Genus Litsea. Mulawarman University Press
Melviani, M., Rohama, R., & Noval, N. 2022. Penggunaan Tanaman Sebagai Obat pada Masyarakatan Suku Banjar, Dayak, dan Bugis di Kalimantan Selatan. Jurnal Surya Medika (JSM): 8(2), 171-177.
Mu'awwanah A dan Ulfah M. 2013. Uji Aktivitas Antioksidan Fraksi n-Heksan Ekstrak Etanol Daun Karika (Carica pubescens) dan Identifikasi Senyawa Alkaloid dan Flavonoidnya. Journal of Chemical Information and Modeling: Vol. 9. - hal. 1689-1699.
Ningsih Ayu Putri, Nurmiati dan Agustien. 2013. Anthoni Uji Aktivitas Antibakteri Ekstrak Kental Tanaman Pisang Kepok Kuning (Musa paradisiaca Linn.) terhadap Staphylococcus aureus dan Escherichia coli. Universitas Andalas Padang : Jurnal Biologi
Noval, N., Kurniawati, D., Rahmadani, R., Budi, S., & Nastiti, K. 2021. Activity and Stability Test of Antiseptic Preparations from The Formulation Combination of Betel Leaf (Piper betle L), Lime Peel (Citrus aurantifolia) and Bundung Plant (Actinoscirpus grossus). In International Conference on Health and Science: (Vol. 1, No. 1, pp. 703-721).
Noval, N., Melviani, M., Novia, N., & Syahrina, D. 2020. Formulasi Dan Evaluasi Sediaan Obat Kumur (Mouthwash) Dari Ekstrak Etanol Tanaman Bundung (Actinoscirpus Grossus) Sebagai Antiseptik Mulut. Jurnal Surya Medika (JSM): 6(1), 112-120.
Noval, N., Yuwindry, I., & Syahrina, D. 2019. Phytochemical Screening and Antimicrobial Activity of Bundung Plants Extract by Dilution Method. Jurnal Surya Medika (JSM): 5(1), 143-154.
Putri Nadya Haqque Santosa. 2019. Aktivitas Antibakteri Ekstrak Tangkai dan Daun Begonia multangula Blume. terhadap Porphyromonas gingivalis. Jurnal Biologi.
Rachmawati dan Umi Dhinarty. 2016. Uji Aktivitas Antibakteri Ekstrak Etanol, Etil Asetat dan Petroleum Eter Rambut Jagung Manis (Zea mays sacarata Strut) terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Malang : Universitas Islam Negeri
Retnowati Yuliana, Bialangi Nurhayati dan Posangi Nona Wingti. 2011. Pertumbuhan Bakteri Staphylococcus aureus pada Media yang Diekspos dengan Infus Daun Sambiloto (Andrographis paniculata). Jurnal Saintek: 6 : Vol. II.
Rohama dan Melviani. 2021. Formulasi dan Evaluasi Sediaan Obat Kumur (Mouthwash) dari Ekstrak Etanol Daun Kalangkala (Litsea angulata) sebagai Antiseptik Mulut. Jurnal Surya Medika (JSM): Vol. I. - hal. 248-256.
Rohama dan Zainuddin Identifikasi Senyawa Metabolit Sekunder pada Ekstrak Daun Gayam (Inocarpus Fagifer Fosb) dengan Menggunakan KLT. Jurnal Surya Medika (JSM: Vol. II. - hal. 125-129.
Sariadi K, Sembiring M dan Litbangkes B Kajian Pustaka: Uji Kepekaan Antibiotik pada Corynebacterium diphtheriae. Jurnal Biotek Medisiana Indonesia: hal. 121-133.
Vonna A dan Nurismi R. 2015. Wound Healing Activity of Unguentum Dosage Form of Ethanol Extracts of Areca catechu L. Nut in Mus musculus albinus. Jurnal Natural Unsyiah: Vol. 2. - hal. 115085.
Warsi dan Sholichah A. 2017. Phytochemical screening and antioxidant activity of ethanolic extract and ethyl acetate fraction from basil leaf (Ocimum basilicum L.) by DPPH radical scavenging method. IOP Conference Series: Materials Science and Engine: 59 : Vol.
Wulandari L. 2011. Kromatografi Lapis Tipis: In Taman Kampus Presindo.
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