Pengaruh Waktu Penundaan Terhadap Hasil Pemeriksaan Serum Glutamic Oxaloacetic Transaminase (SGOT) dengan Metode IFCC (International Federation of Clinical Chemistry) The Effect of Delay Time on Serum Glutamic Oxaloacetic Transaminase (SGOT) Test Results Using the IFCC (International Federation of Clinical Chemistry) Method
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Abstract
Liver damage parameters can be identified from changes in the activity of blood enzymes by observing substances in the blood produced by liver cells, such as Serum Glutamic Oxaloacetic Transaminase (SGOT). In SGOT examinations, delays in the examination can affect the results. As in Good Laboratory Practice, SGOT activity will decrease by 10% in storage for >3 days at 4 °C. The purpose of this study was to determine the effect of delay time on Serum Glutamic Oxaloacetic Transaminase (SGOT) examinations in serum samples stored at 2-8 °C. This study was conducted using an experimental method on 17 randomly selected samples. The SGOT examination was carried out using the IFCC (International Federation of Clinical Chemistry) method using a BTS 350 photometer. The average results of the Serum Glutamic Oxaloacetic Transaminase (SGOT) examination in serum with variations of 0 days (immediately), 3 days, and 7 days were 18.1 U/L, 17.7 U/L, and 17.1 U/L, respectively. Based on the one-way ANOVA test, the significance value was 0.547 (>0.05). This indicates that there is no effect of delay time on the results of the SGOT examination.
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References
Agung, A., Kendran, S., Agung, A., Arjana, G., Agung, A., Istri, S., Patologi, L., Veteriner, K., & Veteriner, L. F. (2017). the the Activities of Alanine Aminotransferase and Aspartate Aminotransferase Enzymes in Male White Rats Treated With Extract Areca Nut Treatment.Buletin Veteriner Udayana, 2017(1), 132–138.
An, B., & Park, C.-E. (2014). Evaluation of Stability of Serum on Different Storage Temperatures for Routine Chemistry Analytes. Korean Journal of Clinical Laboratory Science, 46(4), 111–116.
Departemen Kesehatan Republik Indonesia. 2008. Pedoman Praktik Laboratorium Kesehatan Yang Benar. Jakarta: Departemen Kesehatan RI.
Dufour DR. Liver disease. In:Carl AB, Edward RA, David EB editors. Clinical chemistry and molecular diagnostics. Fourth ed. Missouri: Elsevier saunders; 2006. p. 1777-1827
Kift, R.L., Byrne, C. Liversidge, R. Babbington, F. Knox, C. Binns, J. and Barth, H. 2015. The effect of storage conditions on sample stability in the routine clinical laboratory. 52(6): 675-679
Kurniawan, L. B., Bahrun, U., & Darmawaty. (2012). Hubungan Kadar Transaminase terhadap Mortalitas dan Lama Perawatan Pasien Infark Miokard Correlation between Serum Transaminase and Mortality and Length of Hospitalization in Myocardial Infarction Patients. Jurnal Kedokteran Yarsi, 20(1), 29–35
Netter, Frank H. 2016. Atlas Anatomi Manusia Bahasa Latin/ Indonesia Edisi 6. Indonesia: Elsevier.
Nurmandari, Iswari and Nuryani, Siti and Supriyanta, Bambang (2019) Pengaruh Hemolisis Dalam Serum Terhadap Aktivitas Enzim Alanin Aminotransferase(Alt). thesis, Poltekkes Kemenkes Yogyakarta: Yogyakarta
Pahwa, M.B., Menaka K., Minakshi., Raj M. dan Singh V. 2015. Effect of storage time and temperature on serum clinical biochemistry analytes. 9(4): 150-156
Quiles, J. A., & Chesa, N. R. S. y J. L. B.(2014). Estabilidad de 27 magnitudes bioquímicas en muestras de suero conservadas en refrigeración. 7(1)
Rosida, A. (2016). Pemeriksaan Laboratorium Penyakit Hati. Berkala Kedokteran, 12(1), 123
Sardini, S. (2007). Penentuan Aktivitas Enzim GOT dan GPT dalam Serum dengan Metode Reaksi Kinetik Enzimatik Sesuai IFCC (International Federation of Clinical Chemistry and Laboratory Medicine). Prosiding Pertemuan Dan Presentasi Ilmiah Fungsional Pengembangan Teknologi Nuklir I, 310, 91–106
Tsani RA, Setiani O, Dewanti NAY. 2017. Hubungan Riwayat Pajanan Pestisida dengan Gangguan Fungsi Hati Pada Petani di Desa Sumberejo Kecamatan Ngablak Kabupaten Magelang.
Wanti, Hilda D, Fadhillah, F., & Taufiqurrohman, O. (2020). Journal Of Indonesian Medical Laboratory And Science. Pengaruh Hemolisis Dalam Serum Terhadap Aktivitas Enzim Aspartat Aminotransferase Dengan Metode Kinetik-Ifcc, 116(1), 32–47.