Phytochemical Screening and Antioxidant Activity of Ethanolic Extract of Cawat Hanoman Stem (Bauhinia aculeata L.) using DPPH Method
Article Sidebar
-
Antioxidant, DPPH, Ethanolic extract, Bauhinia aculeata L., Cawat hanoman
Abstract
The free radical is an unstable molecule because contains one or two unpaired electrons. The antioxidant substance is a simple way to decrease the illness caused by free radicals. Cawat hanoman (Bauhinia aculeata L.) was known to contain tannin components one of the benefits as an antioxidant. This research aims to determine the antioxidant activity of the B. aculeata stem tested by qualitatively used thin-layer chromatography (TLC) and quantitatively using the DPPH method. Bauhinia aculeata stem was extracted using a maceration extract method with 96% ethanol. Antioxidant activity test was done qualitatively by eluent of ethyl acetate : methanol : purified water (6 : 2 : 1) using TLC and quantitatively using the DPPH method. The result of antioxidant activity from 96% ethanol extract of B. aculeata stem qualitatively showed the presence of yellow spots on a purple background at TLC after syringed DPPH 0.5 mM and quantitative test that resulted in an IC50 of 21.862 μg/mL. These results indicate that 96% ethanol extract of B. aculeata has very strong antioxidant activity.
Full text article
References
Ichikawa, K., Sasada, R., Chiba, K., & Gotoh, H. (2019). Effect of Side Chain Functional Groups on the DPPH Radical Scavenging Activity of Bisabolane-Type Phenols. Antioxidants, 8(3), 65. doi: 10.3390/antiox8030065
Liochev, S.I. (2013). Reactive oxygen species and the free radical theory of aging. Free Radical Biology and Medicine, 60, 1-4. doi: 10.1016/j.freeradbiomed.2013.02.011
Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118-126. doi: 10.4103/0973-7847.70902
Mahdi-Pour, B., Jothy, S.L., Latha, L.Y., Chen, Y., & Sasidharan, S. (2012). Antioxidant activity of methanol extracts of different parts of Lantana camara. Asian Pacific Journal of Tropical Biomedicine, 2(12), 960–965. doi: 10.1016/S2221-1691(13)60007-6
Margaretta, S., Handayani, S.D., Indraswati, N., & Hindarso, H. (2011). Ekstraksi Senyawa Phenolic Pandanus Amaryllifolius Roxb. sebagai Antioksidan Alami. Widya Teknik, 10(1), 20-30. doi: 10.33508/wt.v10i1.157
Mojzer, E.B., Hrnčič, M.K., Škerget, M., Knez, Z., & Bren, U. (2016). Polyphenols: Extraction Methods, Antioxidative Action, Bioavailability and Anticarcinogenic Effects. Molecules, 21(7), 901. doi: 10.3390/molecules21070901
Mustarichie, R., Runadi, D., & Ramdhani, D. (2017). The Antioxidant Activity and Phytochemical Screening of Ethanol Extract, Fractions of Water, Ethyl Acetate and N-Hexane from Mistletoe Tea (Scurrula atropurpureabl. Dans). Asian Journal of Pharmaceutical and Clinical Research, 10(2), 343-347. doi: 10.22159/ajpcr.2017.v10i2.15724
Muthia, R., Saputri, R., & Verawati, S.A. (2019). Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Mundar (Garcinia forbesii King.) Menggunakan Metode DPPH (2,2-diphenyl-1-picrylhydrazil). Jurnal Pharmascience, 6(1), 74-82. doi: 10.20527/jps.v6i1.6079
Muthia, R. & Wati, H. (2018). Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Kasturi (Mangifera casturi Kosterm.). Jurnal Ilmiah Ibnu Sina, 3(2), 215-223. doi: 10.36387/jiis.v3i2.171
Ningsih, N., Yasni, S., & Yuliani, S. (2017). Sintesis Nanopartikel Ekstrak Kulit Manggis Merah Dan Kajian Sifat Fungsional Produk Enkapsulasinya. Jurnal Teknologi Dan Industri Pangan, 28(1), 27-35. 10.6066/jtip.2017.28.1.27
Pandey, S., Agrawal, R.C., & Maheshwari, S. (2012). In-Vitro Antioxidant and Free Radical Scavenging Activity of Bauhinia Variegata Linn. International Journal of Scientific and Research Publications, 2(2), 1-5.
Pandey, A., Bigoniya, P., Raj, V., & Patel, K.K. (2011). Pharmacological screening of Coriandrum sativum Linn. for hepatoprotective activity. Journal of Pharmacy and BioAllied Sciences, 3(3), 435-441. doi: 10.4103/0975-7406.84462
Phaniendra, A., Jestadi, D.B., & Periyasamy, L. (2015). Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian Journal of Clinical Biochemistry, 30(1), 11-26. doi: 10.1007/s12291-014-0446-0
Pratiwi, D., Wahdaningsih, S., & Isnindar, I. (2013). The Test of Antioxidant Activity from Bawang Mekah Leaves (Eleutherine americana Merr.) using DPPH (2,2-Diphenyl-1-Picrylhydrazyl) Method. Majalah Obat Tradisional, 18(1), 9-16. doi: 10.22146/tradmedj.7755
Sharma, K.R., Kalauni, S.K., & Awale, S. (2015). Antioxidant, Phytotoxic and Antimicrobial Activities of Methanolic Extract of Bauhinia variegata Barks. Journal of Institute of Science and Technology, 20(2), 37-41. doi: 10.3126/jist.v20i2.13946
Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical Screening and Extraction: A Review. Internationale Pharmaceutica Sciencia, 1(1), 98-106.
Tungmunnithum, D., Thongboonyou, A., Pholboon, A., & Yangsabai, A. (2018). Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines, 5(3), 93. doi: 10.3390/medicines5030093
Wati, H., Muthia, R., Jumaryanto, P., Hayati, F., & Rasyida, P.M. (2018). Acute toxicity evaluation of the ethanolic extracts of Bauhinia aculeata L. using organization for economic cooperation and development guideline’s 425. Drug Invention Today, 10(Special issue 5), 3746-3749.
Authors
Copyright (c) 2020 Rahmi Muthia, Muhammad Hidayatullah, Rahmi Hidayati
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors continue to retain the copyright to the article if the article is published in the Borneo Journal of Pharmacy. They will also retain the publishing rights to the article without any restrictions.
Authors who publish in this journal agree to the following terms:
- Any article on the copyright is retained by the author(s).
- The author grants the journal the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share work with an acknowledgment of the work authors and initial publications in this journal.
- Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of published articles (e.g., post-institutional repository) or publish them in a book, with acknowledgment of their initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to and during the submission process. This can lead to productive exchanges and earlier and greater citations of published work.
- The article and any associated published material are distributed under the Creative Commons Attribution-ShareAlike 4.0 International License.
Article Details
