Characterization of Onchidiid Slug (Onchidium typhae) West Kalimantan Waters as Antibacterials and Antifungal
Article Sidebar
Submitted
25 November 2021
Published
28 February 2022
Keywords:
-
Onchidium typhae, Antibacterial, Antifungal, microdilution
Abstract
Onchidiid slug (Onchidium typhae) is a nudibranch that coastal communities in West Kalimantan have widely used as wounds. The study aims to characterize the West Kalimantan water O. typhae as antibacterial and antifungal. The study of O. typhae was carried out in several stages: preparation and optimization, extraction by Quinn method, characterization and identification of bioactive compounds, and antibacterial and antifungal assay using the microdilution method. The result of the proximate test showed that O. typhae powder contains high protein, namely 67.68%. Phytochemical screening results from methanol, ethyl acetate, and chloroform extracts contain alkaloids and amino acids. Methanol, chloroform, and ethyl acetate extract 1% of O. typhae showed inhibitory activity against Staphylococcus aureus, Escherichia coli, and Candida albicans. The most significant inhibition value was indicated by chloroform extract 1%, where the inhibition value against S. aureus, E. coli, and C. albicans was 82±0.01%; 85.8±0.01%; 85±0.01%, respectively. From these results, O. typhae powder can be developed as a wound medicine through its antibacterial and antifungal activity.
Full text article
Generated from XML file
References
1. Chen DL, Zheng W, Feng J, Liu YY, Wang XB. Muscle nutrition composition analysis of three Onchidium species from Hainan Island. Mar Sci. 2017; 41:104-9. doi:10.11759/hykx20170420001
2. Fisch KM, Hertzer C, Böhringer N, Wuisan ZA, Schillo D, Bara R, et al. The Potential of Indonesian Heterobranchs Found around Bunaken Island for the Production of Bioactive Compounds. Mar Drugs. 2017;15(12):384. doi:10.3390/md15120384
3. Shishui H, Xuqian W, Weiming T, Hongkai W, Yongxu C. Biochemical compositon and nutritional evaluation in the muscle of different size Onchidum struma. Oceanol Limnol Sin. 2012;43:43. doi:10.11693/hyhz201204012012
4. Rodriguez J, Riguera R, Debitus C. The natural polypropionate-derived esters of the mollusk Onchidium sp. J Org Chem. 1992; 57(17):4624-32. doi:10.1021/jo00043a018
5. Sun B, Chen C, Shen H, Zhang K, Zhou N, Qian J. Species diversity of Onchidiidae (Eupulmonata: Heterobranchia) on the mainland of China based on molecular data. Molluscan Res. 2014;34(1):62–70. doi:10.1080/13235818.2013.868860
6. Wang B, Chen D, Yu M, Liu Y, Liu P, Zhang X. A Review on Metabolites from Onchidium Genus: Chemistry and Bioactivity. Chem Biodivers. 2021;18(2):e2000580. doi:10.1002/cbdv.202000580
7. Khalifa SAM, Elias N, Farag MA, Chen L, Saeed A, Hegazy MEF, et al. Marine Natural Products: A Source of Novel Anticancer Drugs. Mar Drugs. 2019;17(9):491. doi:10.3390/md17090491
8. Chakraborty K, Joy M. High-value compounds from the molluscs of marine and estuarine ecosystems as prospective functional food ingredients: An overview. Food Res Int. 2020;137:109637. doi:10.1016/j.foodres.2020.109637
9. Tottoli EM, Dorati R, Genta I, Chiesa E, Pisani S, Conti B. Skin Wound Healing Process and New Emerging Technologies for Skin Wound Care and Regeneration. Pharmaceutics. 2020;12(8):735. doi:10.3390/pharmaceutics12080735
10. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound Healing: A Cellular Perspective. Physiol Rev. 2019;99(1):665-706. doi:10.1152/physrev.00067.2017
11. Gonzalez ACdO, Costa TF, Andrade ZdA, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016;91(5):614-20. doi:10.1590/abd1806-4841.20164741
12. Iqbal A, Jan A, Wajid MA, Tariq S. Management of Chronic Non-healing Wounds by Hirudotherapy. World J Plast Surg. 2017;6(1):9-17.
13. Frykberg RG, Banks J. Challenges in the Treatment of Chronic Wounds. Adv Wound Care. 2015;4(9):560-82. doi:10.1089/wound.2015.0635
14. Puca V, Marulli RZ, Grande R, Vitale I, Niro A, Molinaro G, et al. Microbial Species Isolated from Infected Wounds and Antimicrobial Resistance Analysis: Data Emerging from a Three-Years Retrospective Study. Antibiotics. 2021;10(10):1162. doi:10.3390/antibiotics10101162
15. Ekawati ER, Husnul SN, Herawati D. Identifikasi kuman Pada Pus dari Luka Infeksi Kulit. Jq SainHealth. 2018;2(1):31-5. doi:10.51804/jsh.v2i1.174.31-35
16. Hamzah H, Siregar KA, Nurwijayanto A, Wahyuningrum R, Sari S. Effectiveness of Oxalis corniculata L. Ethanol Extract against Mono-Species of Biofilm Staphylococcus aureus. Borneo J Pharm. 2021;4(3):184-91. doi:10.33084/bjop.v4i3.2418
17. Salam AM, Lyles JT, Quave CL. Methods in the Extraction and Chemical Analysis of Medicinal Plants. In: Albuquerque U, de Lucena R, Cruz da Cunha L, Alves R, editors. Methods and Techniques in Ethnobiology and Ethnoecology. Springer Protocols Handbooks. New York (US): Humana Press; 2019. p. 257-83. doi:10.1007/978-1-4939-8919-5_17
18. Helrich K, editor. Official Methods of Analysis of the Association of Official Analytical Chemists. 15th Ed. Virginia (US): Association of Official Analytical Chemist; 1990.
19. Wijianto B, Ritmaleni, Purnomo H, Nurrochmad A. Curcumin mono-carbonyl analogs as potent antibacterial compounds: synthesis, biological evaluation and docking simulation study. Rasayan J Chem. 2020; 13(2):1153–65. doi:10.31788/RJC.2020.1325554
20. Wijianto B, Ritmaleni R, Purnomo H, Nurrochmad A. In silico and in vitro assay of HGV analogue as antibacterial. Int J Pharm Pharm Sci. 2019;11(3):78–85. doi:10.22159/ijpps.2019v11i3.30581
21. Carbone M, Ciavatta ML, Wang JR, Cirillo I, Mathieu V, Kiss R, et al. Extending the record of bis-γ-pyrone polypropionates from marine pulmonate mollusks. J Nat Prod. 2013;76(11):2065-73. doi:10.1021/np400483c
22. Barchitta M, Maugeri A, Favara G, Lio RMS, Evola G, Agodi A et al. Nutrition and Wound Healing: An Overview Focusing on the Beneficial Effects of Curcumin. Int J Mol Sci. 2019;20(5):1119. doi:10.3390/ijms20051119
23. Darmawati D, Hasna C, Fitri A, Munira D. The Effectiveness of High Protein Nutrient to the Post Sectio Caesarea Healing Process. J Medica Vet. 2019;13(2):192-9. doi:10.21157/j.med.vet..v13i2.14090
24. Molnar JA, Underdown MJ, Clark WA. Nutrition and Chronic Wounds. Adv Wound Care. 2014;3(11):663-81. doi:10.1089/wound.2014.0530
25. Huang ZR, Lin YK, Fang JY. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. Molecules. 2009;14(1):540-54. doi:10.3390/molecules14010540
26. Zhao M, Ruan Q, Pan W, Tang Y, Zhao Z, Cui H. New polyketides and diterpenoid derivatives from the fungus Penicillium sclerotiorum GZU-XW03-2 and their anti-inflammatory activity. Fitoterapia. 2020;143:104561. doi:10.1016/j.fitote.2020.104561
27. Ciavatta ML, Lopez-Gresa MP, Gavagnin M, Nicoletti R, Manzo E, Mollo E, et al. Cytosporin-related compounds from the marine-derived fungus Eutypella scoparia. Tetrahedron. 2008;64(22):5365-9. doi:10.1016/j.tet.2008.03.016
28. Ireland C, Faulkner DJ. The defensive secretion of the opisthobranch mollusc Onchidella binneyi. Bioorg Chem. 1978;7(2):125-31. doi:10.1016/0045-2068(78)90043-3
2. Fisch KM, Hertzer C, Böhringer N, Wuisan ZA, Schillo D, Bara R, et al. The Potential of Indonesian Heterobranchs Found around Bunaken Island for the Production of Bioactive Compounds. Mar Drugs. 2017;15(12):384. doi:10.3390/md15120384
3. Shishui H, Xuqian W, Weiming T, Hongkai W, Yongxu C. Biochemical compositon and nutritional evaluation in the muscle of different size Onchidum struma. Oceanol Limnol Sin. 2012;43:43. doi:10.11693/hyhz201204012012
4. Rodriguez J, Riguera R, Debitus C. The natural polypropionate-derived esters of the mollusk Onchidium sp. J Org Chem. 1992; 57(17):4624-32. doi:10.1021/jo00043a018
5. Sun B, Chen C, Shen H, Zhang K, Zhou N, Qian J. Species diversity of Onchidiidae (Eupulmonata: Heterobranchia) on the mainland of China based on molecular data. Molluscan Res. 2014;34(1):62–70. doi:10.1080/13235818.2013.868860
6. Wang B, Chen D, Yu M, Liu Y, Liu P, Zhang X. A Review on Metabolites from Onchidium Genus: Chemistry and Bioactivity. Chem Biodivers. 2021;18(2):e2000580. doi:10.1002/cbdv.202000580
7. Khalifa SAM, Elias N, Farag MA, Chen L, Saeed A, Hegazy MEF, et al. Marine Natural Products: A Source of Novel Anticancer Drugs. Mar Drugs. 2019;17(9):491. doi:10.3390/md17090491
8. Chakraborty K, Joy M. High-value compounds from the molluscs of marine and estuarine ecosystems as prospective functional food ingredients: An overview. Food Res Int. 2020;137:109637. doi:10.1016/j.foodres.2020.109637
9. Tottoli EM, Dorati R, Genta I, Chiesa E, Pisani S, Conti B. Skin Wound Healing Process and New Emerging Technologies for Skin Wound Care and Regeneration. Pharmaceutics. 2020;12(8):735. doi:10.3390/pharmaceutics12080735
10. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound Healing: A Cellular Perspective. Physiol Rev. 2019;99(1):665-706. doi:10.1152/physrev.00067.2017
11. Gonzalez ACdO, Costa TF, Andrade ZdA, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016;91(5):614-20. doi:10.1590/abd1806-4841.20164741
12. Iqbal A, Jan A, Wajid MA, Tariq S. Management of Chronic Non-healing Wounds by Hirudotherapy. World J Plast Surg. 2017;6(1):9-17.
13. Frykberg RG, Banks J. Challenges in the Treatment of Chronic Wounds. Adv Wound Care. 2015;4(9):560-82. doi:10.1089/wound.2015.0635
14. Puca V, Marulli RZ, Grande R, Vitale I, Niro A, Molinaro G, et al. Microbial Species Isolated from Infected Wounds and Antimicrobial Resistance Analysis: Data Emerging from a Three-Years Retrospective Study. Antibiotics. 2021;10(10):1162. doi:10.3390/antibiotics10101162
15. Ekawati ER, Husnul SN, Herawati D. Identifikasi kuman Pada Pus dari Luka Infeksi Kulit. Jq SainHealth. 2018;2(1):31-5. doi:10.51804/jsh.v2i1.174.31-35
16. Hamzah H, Siregar KA, Nurwijayanto A, Wahyuningrum R, Sari S. Effectiveness of Oxalis corniculata L. Ethanol Extract against Mono-Species of Biofilm Staphylococcus aureus. Borneo J Pharm. 2021;4(3):184-91. doi:10.33084/bjop.v4i3.2418
17. Salam AM, Lyles JT, Quave CL. Methods in the Extraction and Chemical Analysis of Medicinal Plants. In: Albuquerque U, de Lucena R, Cruz da Cunha L, Alves R, editors. Methods and Techniques in Ethnobiology and Ethnoecology. Springer Protocols Handbooks. New York (US): Humana Press; 2019. p. 257-83. doi:10.1007/978-1-4939-8919-5_17
18. Helrich K, editor. Official Methods of Analysis of the Association of Official Analytical Chemists. 15th Ed. Virginia (US): Association of Official Analytical Chemist; 1990.
19. Wijianto B, Ritmaleni, Purnomo H, Nurrochmad A. Curcumin mono-carbonyl analogs as potent antibacterial compounds: synthesis, biological evaluation and docking simulation study. Rasayan J Chem. 2020; 13(2):1153–65. doi:10.31788/RJC.2020.1325554
20. Wijianto B, Ritmaleni R, Purnomo H, Nurrochmad A. In silico and in vitro assay of HGV analogue as antibacterial. Int J Pharm Pharm Sci. 2019;11(3):78–85. doi:10.22159/ijpps.2019v11i3.30581
21. Carbone M, Ciavatta ML, Wang JR, Cirillo I, Mathieu V, Kiss R, et al. Extending the record of bis-γ-pyrone polypropionates from marine pulmonate mollusks. J Nat Prod. 2013;76(11):2065-73. doi:10.1021/np400483c
22. Barchitta M, Maugeri A, Favara G, Lio RMS, Evola G, Agodi A et al. Nutrition and Wound Healing: An Overview Focusing on the Beneficial Effects of Curcumin. Int J Mol Sci. 2019;20(5):1119. doi:10.3390/ijms20051119
23. Darmawati D, Hasna C, Fitri A, Munira D. The Effectiveness of High Protein Nutrient to the Post Sectio Caesarea Healing Process. J Medica Vet. 2019;13(2):192-9. doi:10.21157/j.med.vet..v13i2.14090
24. Molnar JA, Underdown MJ, Clark WA. Nutrition and Chronic Wounds. Adv Wound Care. 2014;3(11):663-81. doi:10.1089/wound.2014.0530
25. Huang ZR, Lin YK, Fang JY. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. Molecules. 2009;14(1):540-54. doi:10.3390/molecules14010540
26. Zhao M, Ruan Q, Pan W, Tang Y, Zhao Z, Cui H. New polyketides and diterpenoid derivatives from the fungus Penicillium sclerotiorum GZU-XW03-2 and their anti-inflammatory activity. Fitoterapia. 2020;143:104561. doi:10.1016/j.fitote.2020.104561
27. Ciavatta ML, Lopez-Gresa MP, Gavagnin M, Nicoletti R, Manzo E, Mollo E, et al. Cytosporin-related compounds from the marine-derived fungus Eutypella scoparia. Tetrahedron. 2008;64(22):5365-9. doi:10.1016/j.tet.2008.03.016
28. Ireland C, Faulkner DJ. The defensive secretion of the opisthobranch mollusc Onchidella binneyi. Bioorg Chem. 1978;7(2):125-31. doi:10.1016/0045-2068(78)90043-3
Authors
1.
Wijianto B, Hamzah H, Nurhidayah AL, Kemuning GI, Dyas RAA. Characterization of Onchidiid Slug (Onchidium typhae) West Kalimantan Waters as Antibacterials and Antifungal. Borneo J Pharm [Internet]. 2022Feb.28 [cited 2024Nov.15];5(1):35-41. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/2936
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors continue to retain the copyright to the article if the article is published in the Borneo Journal of Pharmacy. They will also retain the publishing rights to the article without any restrictions.
Authors who publish in this journal agree to the following terms:
- Any article on the copyright is retained by the author(s).
- The author grants the journal the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share work with an acknowledgment of the work authors and initial publications in this journal.
- Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of published articles (e.g., post-institutional repository) or publish them in a book, with acknowledgment of their initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to and during the submission process. This can lead to productive exchanges and earlier and greater citations of published work.
- The article and any associated published material are distributed under the Creative Commons Attribution-ShareAlike 4.0 International License.
Article Details
