Nanoemulsion Mouthwash Formulation of Bajakah Tampala (Spatholobus littoralis Hassk.) Skin Extract Against Candida albicans

Hasyrul Hamzah (1) , Dede Reza Gunawan (2) , Sylvia Utami Tunjung Pratiwi (3) , Muh. Irham Bakhtiar (4) , Virgiawan Yoga Pratama (5) , Muhammad Subhan (6) , Riza Maulana (7)
(1) Universitas Muhammadiyah Kalimantan Timur , Indonesia
(2) Universitas Muhammadiyah Kalimantan Timur , Indonesia
(3) Universitas Gadjah Mada , Indonesia
(4) Universitas Muhammadiyah Kalimantan Timur , Indonesia
(5) Universitas Muhammadiyah Kalimantan Timur , Indonesia
(6) Khon Kaen University , Thailand
(7) Muhammadiyah University of Surakarta , Indonesia

Abstract

Candida albicans can cause two infections in humans: superficial and systemic. The ability of C. albicans to infect the host is influenced by virulence factors and character changes so that it can fool the immune system. From the character change factor, C. albicans can form a biofilm. This study aims to determine the good concentration in inhibiting and determine the antifungal and antibiofilm activity of nanoemulsion mouthwash formulation of bajakah tampala (Spatholobus littoralis Hassk) skin extract against C. albicans. This research was conducted with an experimental method. The formulation used a spontaneous magnetic stirrer technique to make nanoemulsion preparations. Antifungal and antibiofilm tests were carried out by dilution method using a 96-well plate and a microplate reader with a wavelength of 620 nm to determine the percentage inhibition against C. albicans and determine MIC50 and MBIC50. The results showed that the nanoemulsion mouthwash formulation of S. littoralis inhibited the planktonic and biofilm of C. albicans. The concentration of 1% is expressed as MIC50 and MBIC50. Therefore, the nanoemulsion formulation of S. littoralis extract could inhibit the growth of C. albicans in the oral cavity.

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Authors

Hasyrul Hamzah
hh241@umkt.ac.id (Primary Contact)
Dede Reza Gunawan
Sylvia Utami Tunjung Pratiwi
Muh. Irham Bakhtiar
Virgiawan Yoga Pratama
Muhammad Subhan
Riza Maulana
Author Biographies

Hasyrul Hamzah, Universitas Muhammadiyah Kalimantan Timur

Department of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan, Indonesia

Indonesian Biofilm Research Collaboration Center, Sleman, Special Region of Yogyakarta, Indonesia

Dede Reza Gunawan, Universitas Muhammadiyah Kalimantan Timur

Department of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan, Indonesia

Indonesian Biofilm Research Collaboration Center, Sleman, Special Region of Yogyakarta, Indonesia

Sylvia Utami Tunjung Pratiwi, Universitas Gadjah Mada

Department of Pharmaceutical Biology, Universitas Gadjah Mada, Sleman, Special Region of Yogyakarta, Indonesia

Indonesian Biofilm Research Collaboration Center, Sleman, Special Region of Yogyakarta, Indonesia

Muh. Irham Bakhtiar, Universitas Muhammadiyah Kalimantan Timur

Department of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan, Indonesia

Indonesian Biofilm Research Collaboration Center, Sleman, Special Region of Yogyakarta, Indonesia

Virgiawan Yoga Pratama, Universitas Muhammadiyah Kalimantan Timur

Department of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan, Indonesia

Indonesian Biofilm Research Collaboration Center, Sleman, Special Region of Yogyakarta, Indonesia

Muhammad Subhan, Khon Kaen University

Master of Sciences Program In Pharmaceutical Chemistry and Natural Product, Khon Kaen University, Khon Kaen, Khon Kaen, Thailand

Riza Maulana, Muhammadiyah University of Surakarta

Department of Pharmacy, Universitas Muhammadiyah Surakarta, Surakarta, Central Java, Indonesia

1.
Hamzah H, Gunawan DR, Pratiwi SUT, Bakhtiar MI, Pratama VY, Subhan M, Maulana R. Nanoemulsion Mouthwash Formulation of Bajakah Tampala (Spatholobus littoralis Hassk.) Skin Extract Against Candida albicans . Borneo J Pharm [Internet]. 2024Feb.29 [cited 2024May24];7(1):1-13. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/5548

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