Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava)

Mohammad Rizki Fadhil Pratama (1) , Suratno Suratno (2) , Evi Mulyani (3)
(1) Universitas Muhammadiyah Palangkaraya , Indonesia
(2) Universitas Muhammadiyah Palangkaraya , Indonesia
(3) Universitas Muhammadiyah Palangkaraya , Indonesia

Abstract

This study aims to obtain the profile of Thin-Layer Chromatography (TLC) and Ultraviolet-Visible (UV-Vis) spectrophotometry from the ethanol extract of akar kuning stems (Arcangelisia flava) from Central Kalimantan. The TLC method is used with the orientation phase of the combination of polar-non-polar solvents resulting from orientation, while ethanol is used as the solvent for UV-Vis spectrophotometers. TLC results showed the formation of three stains on a mixture of polar solvents chloroform : methanol : water while in a non-polar solvent combination n-hexane : ethyl acetate did not show any stains. Comparison of retention factor (Rf) values shows the best combination of polar solvents to separate stains at a ratio of 5 : 2 : 1, respectively. Separation in 2-dimensional TLC with polar solvents showed a similar pattern with 1-dimensional separation in the form of three stains. UV-Vis spectrophotometer results showed four main peaks with wavelength 227.2; 267.4; 345.2; and 425.3 nm, respectively. The profile of the peak formed is very similar to that shown by berberine, one of the primary metabolites of akar kuning. TLC and UV-Vis spectrophotometers profiles obtained are expected to support further research using akar kuning stems, especially those from Central Kalimantan.

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References

Abarca-Vargas, R., Malacara, C.F.P., Petricevich, C.L. 2016. Characterization of Chemical Compounds with Antioxidant and Cytotoxic Activities in Bougainvillea x buttiana Holttum and Standl, (var. Rose) Extracts. Antioxidants. 5(4):pii:E45.
Azwanida, N.N. 2015. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal & Aromatic Plants. 4(3):1000196.
Behera, S., Ghanty, S., Ahmad, F., Santra, S., Banerjee, S. 2012. UV-Visible Spectrophotometric Method Development and Validation of Assay of Paracetamol Tablet Formulation. Journal of Analytical & Bioanalytical Techniques. 3(6):1000151.
Bele, A.A., Khale, A. 2011. An overview on thin layer chromatography. International Journal of Pharmaceutical Sciences and Research. 2(2):256-267.
Coskun, O. 2016. Separation techniques: Chromatography. Northern Clinics of Istanbul. 3(2):156-160.
Dai, J., Mumper, R.J. 2010. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 15(10):7313-7352.
Desmukh, S.A., Gaikwad, D.K. 2014. A review of the taxonomy, ethnobotany, phytochemistry and pharmacology of Basella alba (Basellaceae). Journal of Applied Pharmaceutical Science. 4(1):153-165.
Fahrianoor, Windari, T., Taharuddin, Ruslimar’I, Maryono. 2013. The Practice of Local Wisdom of Dayak People in Forest Conservation in South Kalimantan. Journal of Wetlands Environment Management. 1(1):33-41.
Guerriero, G., Berni, R., Munoz-Sanchez, J.A., Apone, F., Abdel-Salam, E.M., Qahtan, A.A., Alatar, A.A., Cantini, C., Cai, G., Hausman, J.F., Siddiqui, K.S., Hernandez-Sotomayor, S.M., Faisal, M. 2018. Review: Production of plant secondary metabolites: examples, tips and suggestions for biotechnologists. Genes. 9(6):pii:E309.
Haug, M. 2014. Resistance, Ritual Purification and Mediation: Tracing a Dayak Community's Sixteen-Year Search for Justice in East Kalimantan. The Asia Pacific Journal of Anthropology. 15(4):357-375.
Kagan, I.A., Flythe, M.D. 2014. Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds. Journal of Visualized Experiments. (85):51411.
Kaijanen, L., Paakkunainen, M., Pietarinen, S., Jernstrom, E., Reinikainen, S.P. 2015. Ultraviolet Detection of Monosaccharides: Multiple Wavelength Strategy to Evaluate Results after Capillary Zone Electrophoretic Separation. International Journal of Electrochemical Science. 10:2950-2961.
Koide, T., Iwata, M., Maekawa, K., Saito, H., Tanimoto, T., Okada, S. 2001. [Berberine hydrochloride reference standard (Control 001) of National Institute of Health Sciences]. Kokuritsu Iyakuhin Shokuhin Eiken Kenkyusho Hokoku. 119:97-100.
Kolb, C.A., Kaser, M.A., Kopecky, J., Zotz, G., Riederer, M., Pfundel, E.E. 2001. Effects of natural intensities of visible and ultraviolet radiation on epidermal ultraviolet screening and photosynthesis in grape leaves. Plant Physiology. 127(3):863-875.
Maryani, Marsoedi, Nursyam, H., & Maftuch. 2013. The Phytochemistry and The Anti-Bacterial Activity of Yellow Root (Arcangelisia flava Merr) against Aeromonas hydrophila. Journal of Biology and Life Science. 4(2):180-190.
Matsyik, E., Wozniak, A., Paduch, R., Rejdak, R., Polak, B., Donica, H. 2016. The new TLC method for separation and determination of multicomponent mixtures of plant extracts. Journal of Analytical Methods in Chemistry. 1813581.
Pratama, M.R.F. 2016. Akar kuning (Arcangelisia flava) sebagai inhibitor EGFR: Kajian in silico. Jurnal Farmagazine. 3(1):6-16.
Pratama, M.R.F. 2017. Akar Kuning (Arcangelisia flava) as Neuraminidase Inhibitor: Molecular Docking and Pharmacophore Optimization Approach. Advances in Health Sciences Research. 6:502-511.
Pratama, M.R.F., Pratomo, G.S. 2017. Pharmacophore optimization of berberine as HER2 inhibitor. Jurnal Farmasi Indonesia. 14(2):109-117.
Pundarikakshudu, K., Dave, H.N. 2010. Simultaneous determination of curcumin and berberine in their pure form and from the combined extracts of Curcuma longa and Berberis aristata. International Journal of Applied Science and Engineering. 8(1):19-26.
Setyowati, F.M. 2010. Etnofarmakologi dan Pemakaian Tanaman Obat Suku Dayak Tunjung di Kalimantan Timur. Media Litbang Kesehatan. 20(3):104-112.
Stoll, D.R., Li, X., Wang, X., Carr, P.W., Porter, S.E.G., Rutan, S.C. 2011. Fast, comprehensive two dimensional liquid chromatography. Journal of Chromatography A. 1168(1-2):3-2.
Subiandono, E. & Heriyanto, N.M. 2009. Kajian Tumbuhan Obat Akar Kuning (Arcangelisia flava Merr.) di Kelompok Hutan Gelawan, Kabupaten Kampar, Riau. Buletin Plasma Nutfah. 15(1):43-48.
Wahyuono, S., Setiadi, J., Santosa, D., Hartati, M.S., Soekotjo, Muslimah, S., Prihatiningtias, W. 2006. Identifikasi Struktur Senyawa Bioaktif dari Akar Kuning (Fibraurea chloroleuca Miers.) (#03-SBK-029) Koleksi dari Hutan Kalimantan Tengah. Majalah Obat Tradisional. 12(39):1-8.
Wang, H., Zhu, C., Ying, Y., Luo, L., Huang, D., Luo, Z. 2018. Metformin and berberine, two versatile drugs in treatment of common metabolic diseases. Oncotarget. 9(11):10135-10146.
Widi, R.K., Indriati, T. 2007. Penjaringan dan Identifikasi Senyawa Alkaloid dalam Batang Kayu Kuning (Arcangelisia flava Merr). Jurnal Ilmu Dasar. 8(1):24-29.

Authors

Mohammad Rizki Fadhil Pratama
mohammadrizkifadhilpratama@gmail.com (Primary Contact)
Suratno Suratno
Evi Mulyani
Author Biographies

Mohammad Rizki Fadhil Pratama, Universitas Muhammadiyah Palangkaraya

Suratno Suratno, Universitas Muhammadiyah Palangkaraya

Evi Mulyani, Universitas Muhammadiyah Palangkaraya

1.
Pratama MRF, Suratno S, Mulyani E. Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava). Borneo J Pharm [Internet]. 2018Nov.1 [cited 2024Nov.7];1(2):72-6. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/367

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