Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava)

Mohammad Rizki Fadhil Pratama (1) , Suratno Suratno (2) , Evi Mulyani (3)
(1) Universitas Muhammadiyah Palangkaraya , Indonesia
(2) Universitas Muhammadiyah Palangkaraya , Indonesia
(3) Universitas Muhammadiyah Palangkaraya , Indonesia


This study aims to obtain the profile of Thin-Layer Chromatography (TLC) and Ultraviolet-Visible (UV-Vis) spectrophotometry from the ethanol extract of akar kuning stems (Arcangelisia flava) from Central Kalimantan. The TLC method is used with the orientation phase of the combination of polar-non-polar solvents resulting from orientation, while ethanol is used as the solvent for UV-Vis spectrophotometers. TLC results showed the formation of three stains on a mixture of polar solvents chloroform : methanol : water while in a non-polar solvent combination n-hexane : ethyl acetate did not show any stains. Comparison of retention factor (Rf) values shows the best combination of polar solvents to separate stains at a ratio of 5 : 2 : 1, respectively. Separation in 2-dimensional TLC with polar solvents showed a similar pattern with 1-dimensional separation in the form of three stains. UV-Vis spectrophotometer results showed four main peaks with wavelength 227.2; 267.4; 345.2; and 425.3 nm, respectively. The profile of the peak formed is very similar to that shown by berberine, one of the primary metabolites of akar kuning. TLC and UV-Vis spectrophotometers profiles obtained are expected to support further research using akar kuning stems, especially those from Central Kalimantan.

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Mohammad Rizki Fadhil Pratama (Primary Contact)
Suratno Suratno
Evi Mulyani
Author Biographies

Mohammad Rizki Fadhil Pratama, Universitas Muhammadiyah Palangkaraya

Suratno Suratno, Universitas Muhammadiyah Palangkaraya

Evi Mulyani, Universitas Muhammadiyah Palangkaraya

Pratama MRF, Suratno S, Mulyani E. Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava). Borneo J Pharm [Internet]. 2018Nov.1 [cited 2024Jun.14];1(2):72-6. Available from:

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