Chemical Screening and Antioxidant Activity of Ethyl Acetate Fraction and Residue from Lansium domesticum Ethanolic Extract
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GC-MS Profile, Antioxidant activity, SPF Value, Lansium domesticum
Abstract
Langsat (Lansium domesticum) is a plant that thrives in the tropics. The search for photoprotective compounds derived from natural ingredients has been intensively conducted to develop cosmetic formulations to obtain safe and protective products against UV rays. This study aims to identify chemical compounds in the ethyl acetate fraction and residues of ethanol extract from L. domesticum bark using tube and gas chromatography-mass spectrophotometry tests. At the same time, the antioxidant activity and SPF values were assessed with the DPPH and FRAP methods. The qualitative GC-MS test obtained the percentage peak value and molecular weight. Furthermore, the antioxidant activity was tested using the compound DPPH measured with an ELISA reader at a wavelength of 515.5 nm with UV/vis spectrophotometer and FRAP method using FeCl3 and TPTZ compounds at 615 nm. The SPF value was assessed using Mansur's formula with UV/Vis spectrophotometer at 290 to 320 nm. The results obtained in the ethyl acetate fraction showed terpenoids, while the residue contains phenolics, flavonoids, tannins, and saponins. The GC-MS tests found that the ethyl acetate fraction had 47 types of chemical compounds, including terpenoids and fatty acids group; in the residue, four classes were found, with terpenoid and fatty acid groups being more predominant. Based on the results, the ethyl acetate fraction has an IC50 value of 341.25±26.45 µg/ml and 436.3±10.8 µg/ml; the residue was 94.72±34.22 µg/ml and 2602.79±11.8 µg/ml. Additionally, the SPF values for both were 2.87 and 3.9.
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References
2. Tilaar M, Wih WL, Ranti AS, Wasitaatmadja SM, Suryaningsih S, Junardy FD, et al. Review of lansium domesticum corrêa and its use in cosmetics. Bol Latinoam y del Caribe Plantas Med y Aromat. 2008;7(4):183–9.
3. Abdallah HM, Mohamed GA, Ibrahim SRM. Lansium domesticum—A Fruit with Multi-Benefits: Traditional Uses, Phytochemicals, Nutritional Value, and Bioactivities. Nutrients. 2022;14(7):1531. doi:10.3390/nu14071531
4. Apridamayanti P, Fajriaty I, Hatita E. Antioxidant activity and analgesic assessment of Lansium domesticum stem bark infusion. Nusantara Biosci. 2018;10(2):71–5. doi:10.13057/nusbiosci/n100201
5. Worang RL, Semuel MY, Pendong DF. Anti malarial and Antibacterial Bioactivity of Langsat (Lansium minahasae L.) Bark Extract. J Nat Sci Res. 2013;3(4):1–11.
6. Yamin Y, Ruslin R, Sabarudin S, Sida NA, Kasmawati H, Diman LOM. Determination of Antiradical Activity, Total Phenolic, and Total Flavonoid Contents of Extracts and Fractions of Langsat (Lansium domesticum Coor.) Seeds. Borneo J Pharm. 2020;3(4):249-56. doi:10.33084/bjop.v3i4.1500
7. Mota MD, Morte ANdB, Silva LCRCE, Chinalia FA. Sunscreen protection factor enhancement through supplementation with Rambutan (Nephelium lappaceum L) ethanolic extract. J Photochem Photobiol B Biol. 2020;205:111837. doi:10.1016/j.jphotobiol.2020.111837
8. Hoang HT, Moon JY, Lee YC. Natural Antioxidants from Plant Extracts in Skincare Cosmetics: Recent Applications, Challenges and Perspectives. Cosmetics. 2021;8(4):106. doi:10.3390/cosmetics8040106
9. Faccio G. Plant Complexity and Cosmetic Innovation. iScience. 2020;23(8):101358. doi:10.1016/j.isci.2020.101358
10. Giri DP, Rajbhandari M. Phytochemical Analysis and Constituents of Hexane Extract of Melastoma Malabathricum L. J Inst Sci Technol. 2018;23(1):18–25. doi:10.3126/jist.v23i1.22150
11. Maesaroh K, Kurnia D, Al Anshori J. Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC Terhadap Asam Askorbat, Asam Galat dan Kuersetin. Chim Nat Acta. 2018;6(2):93-100. doi:10.24198/cna.v6.n2.19049
12. Nur S, Rumiyati R, Lukitaningsih E. Screening of Antioxidants, Anti-Aging and Tyrosinase Inhibitory Activities of Ethanolic and Ethyl Acetate Extracts of Fruit Flesh and Fruit Peel Langsat (Lansium domesticum Corr) In Vitro. Majalah Obat Tradisional. 2017;22(1):63-72. doi:10.22146/tradmedj.24342
13. Mansur JS, Breder MNR, Mansur MCA, Azulay RD. Determinação Do Fator De Proteção Solar Por Espectrofotometria. An Bras Dermatol Rio De Janeiro. 1986;61:121-4.
14. Lim TK. Edible Medicinal and Non Medicinal Plants: Volume 3, Fruits. Dordrecht: Springer; 2012. doi:10.1007/978-94-007-2534-8
15. Bülow N, König WA. The role of germacrene D as a precursor in sesquiterpene biosynthesis: Investigations of acid catalyzed, photochemically and thermally induced rearrangements. Phytochemistry. 2000;55(2):141–68. doi:10.1016/s0031-9422(00)00266-1
16. Herrera-Calderon O, Chavez H, Enciso-Roca EC, Común-Ventura PW, Hañari-Quispe RD, Figueroa-Salvador L, et al. GC-MS Profile, Antioxidant Activity, and In Silico Study of the Essential Oil from Schinus molle L. Leaves in the Presence of Mosquito Juvenile Hormone-Binding Protein (mJHBP) from Aedes aegypti. Biomed Res Int. 2022;2022:5601531. doi:10.1155/2022/5601531
17. Mozaffari E, Abai MR, Khanavi M, Vatandoost H, Sedaghat MM, Moridnia A, et al. Chemical composition, larvicidal and repellency properties of cionura erecta (L.) Griseb. against malaria vector, Anopheles stephensi liston (Diptera: Culicidae). J Arthropod Borne Dis. 2014;8(2):147–55.
18. Hanum L, Kasiamdari RS. Tumbuhan Duku: Senyawa Bioaktif, Aktivitas Farmaklogis dan Prospeknya dalam Bidang Kesehatan. J Biol Papua. 2018;5(2):84–93. doi:10.31957/jbp.528
19. Liu Z, Ren Z, Zhang J, Chuang CC, Kandaswamy E, Zhou T, et al. Role of ROS and Nutritional Antioxidants in Human Diseases. Front Physiol. 2018;9:477. doi:10.3389/fphys.2018.00477
20. Lubis MF, Hasibuan PA, Syahputra H, Astyka R. A Review on Phytochemicals and Pharmacological Activities as Ethnomedicinal Uses of Duku (Lansium domesticum Corr.). Open Access Maced J Med Sci. 2022;10(F):57–65. doi:10.3889/oamjms.2022.8394
21. Munteanu IG, Apetrei C. Analytical methods used in determining antioxidant activity: A review. Int J Mol Sci. 2021;22(7):3380. doi:10.3390/ijms22073380
22. Rodríguez-Bonilla P, Gandía-Herrero F, Matencio A, García-Carmona F, López-Nicolás JM. Comparative Study of the Antioxidant Capacity of Four Stilbenes Using ORAC, ABTS+, and FRAP Techniques. Food Anal Methods. 2017;10(9):2994–3000. doi:10.1007/s12161-017-0871-9
23. Graßmann J. Terpenoids as Plant Antioxidants. Vitam Horm. 2005;72:505–35. doi:10.1016/s0083-6729(05)72015-x
24. Apridamayanti P, Pratiwi L, Sari R. Gas Chromatography Study of N-Hexane and Chloroform Fractions of Ethanol Extract of Melastoma Malabathricum L. Leaves: an in Vitro Study of Antioxidant and Spf Values. Int J Appl Pharm. 2022;14(3):40–6. doi:10.22159/ijpps.2022v14i3.43801
25. Parit SB, Dawkar V V., Tanpure RS, Pai SR, Chougale AD. Nutritional Quality and Antioxidant Activity of Wheatgrass (Triticum aestivum) Unwrap by Proteome Profiling and DPPH and FRAP assays. J Food Sci. 2018;83(8):2127–39. doi:10.1111/1750-3841.14224
26. Sethi S, Joshi A, Arora B, Bhowmik A, Sharma RR, Kumar P. Significance of FRAP, DPPH, and CUPRAC assays for antioxidant activity determination in apple fruit extracts. Eur Food Res Technol. 2020;246(3):591–8. doi:10.1007/s00217-020-03432-z
27. Mucha P, Skoczyńska A, Małecka M, Hikisz P, Budzisz E. Overview of the Antioxidant and Anti-Inflammatory Activities of Selected Plant Compounds and Their Metal Ions Complexes. Molecules. 2021;26(16):4886. doi:10.3390/molecules26164886
28. Rodrigues NDN, Staniforth M, Stavros VG. Photophysics of sunscreen molecules in the gas phase: a stepwise approach towards understanding and developing next-generation sunscreens. Proc Math Phys Eng Sci. 2016;472(2195):20160677. doi:10.1098/rspa.2016.0677
29. Donglikar MM, Deore SL. Sunscreens: A review. Pharmacogn J. 2016;8(3):171-9. doi:10.5530/pj.2016.3.1
30. Cotas J, Leandro A, Monteiro P, Pacheco D, Figueirinha A, Gonçalves AMM, et al. Seaweed Phenolics: From Extraction to Applications. Mar Drugs. 2020;18(8):384. doi:10.3390/md18080384
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