Phytochemical Analysis and Anti-Inflammatory Activity of The Combination of Trigona apicalis propolis Extract and Honey
DOI:
https://doi.org/10.33084/bjop.v6i2.4696Keywords:
Inflammatory, Propolis, Combination, In vivo, East KalimantanAbstract
Chronic inflammation is common in infectious diseases, rheumatoid arthritis, gout, and autoimmune diseases. However, using non-steroidal anti-inflammatory drugs (NSAIDs) is accompanied by dangerous side effects. Therefore, searching for safer alternative therapies without side effects is very important. A natural blend of ingredients produced by stingless bees from plants was potential as a remedy. Meanwhile, the potential of kelulut bee products from East Kalimantan as an anti-inflammatory is still unknown. This study aimed to compare the chemical composition of kelulut bee (Trigona apicalis) products and evaluate the anti-inflammatory effect of honey, propolis, and their combination. Propolis extract and honey were determined as secondary metabolites. An anti-inflammatory in vivo assay triggered the edema using carrageenan on male mice and measured its anti-inflammatory power value. Propolis extract and honey from T. apicalis have a promising anti-inflammatory effect and are significantly higher than the positive control. Meanwhile, combining propolis extract and honey did not enhance the anti-inflammatory effect. In addition, combining honey and propolis preparations with a ratio of 75 : 25 has a better effect on reducing edema volume than the other two combinations. Still, it is not better than the treatment with propolis extract or honey alone. The content of polyphenol compounds found in honey and propolis preparations is thought to have an important role in reducing edema volume.
Downloads
References
Michles N, van Aart C, Morisse J, Mullee A, Huybrechts I. Chronic inflammation towards cancer incidence: A systematic review and meta-analysis of epidemiological studies. Crit Rev Oncol Hematol. 2021;157:103177. doi:10.1016/j.critrevonc.2020.103177
Arozal W, Louisa M, Soetikno V. Selected Indonesian Medicinal Plants for the Management of Metabolic Syndrome: Molecular Basis and Recent Studies. Front Cardiovasv Med. 2020;7:82. doi:10.3389/fcvm.2020.00082
Syafizal, Bratawinata AA, Sila M, Marji D. Jenis Lebah Kelulut (Trigona spp.) di Hutan Pendidikan Lempake. Mulawarman Sci. 2012;11(1):11-8.
Goh LPW, Jawan R, Faik AAM, Gansau JA. A review of stingless bees' bioactivity in different parts of the world. J Med Life. 2023;16(1):16-21. doi:10.25122/jml-2022-0160
Pasupuleti VR, Sammugam L, Ramesh N, Gan SH. Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits. Oxid Med Cell Longev. 2017;2017:1259510. doi:10.1155/2017/1259510
Wagh VD. Propolis: a wonder bees product and its pharmacological potentials. Adv Pharmacol Sci. 2013;2013:308249. doi:10.1155/2013/308249
Bachevski D, Damevska K, Simeonovski V, Dimova M. Back to the basics: Propolis and COVID‐19. Dermatol Ther. 2020;33(4):e13780. doi:10.1111/dth.13780
Mulyati AF, Sulaeman A, Marliyati SA, Rafi M, Rokhmah UF. Macro and Micronutrient Content of Raw Propolis Collected from Different Regions in Indonesia. J Gizi Pangan. 2021;16(Suppl 1):109-14.
Syafrizal, Ramadhan R, Kusuma IW, Egra S, Shimizu K, Kanzaki M, et al. Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiversitas. 2020;21(10):4623-30. doi:10.13057/biodiv/d211021
Rozman AS, Hashim N, Maringgal B, Abdan K. A Comprehensive Review of Stingless Bee Products: Phytochemical Composition and Beneficial Properties of Honey, Propolis, and Pollen. Appl Sci. 2022;12(13):6370. doi:10.3390/app12136370
Dermiati T, Natalia CK, Tandi J. Uji Antiinflamasi Ekstrak Etanol Kulit Batang Ceremai (Phyllanthus acidus L.Skell) terhadap Edema Kaki Tikus. Farmakologika J Farmasi. 2018;15(1):98-105.
Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs. Proc Soc Exp Biol Med. 1962;111:544-7. doi:10.3181/00379727-111-27849
Fadzilah NH, Jaapar MF, Jajuli R, Omar WAW. Total phenolic content, total flavonoid and antioxidant activity of ethanolic bee pollen extracts from three species of Malaysian stingless bee. J Apic Res. 2017;56(2):130-5. doi:10.1080/00218839.2017.1287996
Kustiawan PM, Aziz A, Yuliawan VN. Antioxidant and Antibacterial Activity of Various Fractions of Heterotrigona itama Propolis Found in Kutai Kartanegara. Open Access Maced J Med Sci. 2022;10(A):531-4. doi:10.3889/oamjms.2022.7766
Ti H, Zhuang Z, Yu Q, Wang S. Progress of Plant Medicine Derived Extracts and Alkaloids on Modulating Viral Infections and Inflammation. Drug Des Devel Ther. 2021;15:1385-408. doi:10.2147/dddt.s299120
Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. ScientificWorldJournal. 2013;2013:162750. doi:10.1155/2013/162750
Divekar PA, Narayana S, Divekar BA, Kumar R, Gadratagi BG, Ray A, et al. Plant Secondary Metabolites as Defense Tools against Herbivores for Sustainable Crop Protection. Int J Mol Sci. 2022;23(5):2690. doi:10.3390/ijms23052690
Fraga-Corral M, Garcia-Oliveira P, Pereira AG, Lourenco-Lopes C, Jimenez-Lopez C, Proeto MA, et al. Technological application of tannin-based extract. Molecules. 2020;25(3):614. doi:10.3390/molecules25030614
Patil KR, Mahajan UB, Unger BS, Goyal SN, Belemkar S, Surana SJ, et al. Animal Models of Inflammation for Screening of Anti-inflammatory Drugs: Implications for the Discovery and Development of Phytopharmaceuticals. Int J Mol Sci. 2019;20(18):4367. doi:10.3390/ijms20184367
Murtaza G, Karim S, Akram MR, Khan SA, Azhar S, Mumtaz A, et al. Caffeic acid phenethyl ester and therapeutic potentials. Biomed Res Int. 2014;2014:145342. doi:10.1155/2014/145342
Pahlavani N, Malekahmadi M, Firouzi S, Rostami D, Sedaghat A, Moghadam AB, et al. Molecular and cellular mechanisms of the effects of Propolis in inflammation, oxidative stress and glycemic control in chronic diseases. Nutr Metab. 2020;17:65. doi:10.1186/s12986-020-00485-5
Olas B. Honey and Its Phenolic Compounds as an Effective Natural Medicine for Cardiovascular Diseases in Humans? Nutrients. 2020;12(2):283. doi:10.3390/nu12020283
Samarghandian S, Farkhondeh T, Samini F. Honey and health: a review of recent clinical research. Pharmacognosy Res. 2017;9(2):121-7. doi:10.4103/0974-8490.204647
Eteraf-Oskouei T, Najafi M. Traditional and modern uses of natural honey in human diseases: a review. Iran J Basic Med Sci. 2013;16(6):731-42.
Esa NEF, Ansari MNM, Abd Razak SI, Ismail NI, Jusoh N, Zamawi NA, et al. A review on recent progress of stingless bee honey and its hydrogel-based compound for wound care management. Molecules. 2022;27(10):3080. doi:10.3390/molecules27103080
Downloads
Published
Issue
Section
Categories
License
Copyright (c) 2023 Paula Mariana Kustiawan, Chaerul Fadly Mochtar Luthfi M, Sinta Ratna Dewi, Jati Pratiwi, Novia Misnawati Aisyiyah, Alfin Syahrian Dwi Nugraha, Irfan Muris Setiawan
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors continue to retain the copyright to the article if the article is published in the Borneo Journal of Pharmacy. They will also retain the publishing rights to the article without any restrictions.
Authors who publish in this journal agree to the following terms:
- Any article on the copyright is retained by the author(s).
- The author grants the journal the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share work with an acknowledgment of the work authors and initial publications in this journal.
- Authors can enter into separate, additional contractual arrangements for the non-exclusive distribution of published articles (e.g., post-institutional repository) or publish them in a book, with acknowledgment of their initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to and during the submission process. This can lead to productive exchanges and earlier and greater citations of published work.
- The article and any associated published material are distributed under the Creative Commons Attribution-ShareAlike 4.0 International License.