Standardized Herbal Extract for Wound Healing: A Comparative Study of Centella asiatica, Curcuma domestica, and Heterotrigona itama Honey Combination in Rabbits
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Submitted
20 September 2023
20 September 2023
Published
30 November 2024
30 November 2024
Abstract
Centella asiatica and Curcuma domestica are recognized for their wound-healing properties. This study investigated the synergistic effects of combining these plant extracts with stingless bee (Heterotrigona itama) honey on wound healing in rabbits. Ethanolic extracts of C. asiatica and C. domestica were prepared in a 1 : 10 ratio. Blended formulations containing 20% plant extracts and 80% honey were created in two ratios: 1 : 1 and 2 : 1. The total flavonoid and tannin content of the plant extracts were quantified. Male rabbits were subjected to a wound-healing model, and the formulations were applied topically for seven days. Wound healing was assessed, and the results were compared to a positive control. The ethanolic extracts of C. asiatica and C. domestica were rich in flavonoids and tannins. The blended honey formulations exhibited promising wound-healing effects, with Formula 2 (2 : 1 ratio of plant extracts) demonstrating slightly superior results compared to Formula 1 and the positive control. These findings suggest the potential of the combined extracts of C. asiatica, C. domestica, and H. itama honey as effective wound-healing agents.
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18. Mishra B. Assessment of plant diversity: a medicinal, conservational and environmental study. Asian J Biol. 2023;18(1):39–57. DOI: 10.9734/ajob/2023/v18i1336
19. Nakatani N. Phenolic antioxidants from herbs and spices. Biofactors. 2000;13(1-4):141–6. DOI: 10.1002/biof.5520130123; PMID: 11237173
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21. Swain SS, Sahoo G, Mahapatra PK, Panda SK. Disease burden and current therapeutical status of leprosy with special emphasis on phytochemicals. Curr Top Med Chem. 2022;22(19):1611–25. DOI: 10.2174/1568026621666210909162435
22. Taghizadeh M, Jalili S. Phytochemical content, antioxidant properties, and antibacterial activities of Centella asiatica L. Nat Prod Res. 2024;38(20):3693-8. DOI: 10.1080/14786419.2023.2258439; PMID: 37708329
23. Fahryl N, Carolia N. Kunyit (Curcuma domestica Val) sebagai Terapi artritis gout. Majority Med J Lampung Univ. 2019;8(1):251–5.
24. Esa NEF, Ansari MNM, Razak SIA, Ismail NI, Jusoh N, Zawawi NA, et al. A Review on Recent Progress of Stingless Bee Honey and Its Hydrogel-Based Compound for Wound Care Management. Molecules. 2022;27(10):3080. DOI: 10.3390/molecules27103080; PMCID: PMC9145090; PMID: 35630557
25. Kustiawan PM, Hanifa DNC, Nugraha ASD, Suwandi A, Monica A, Agustinur A. Edukasi dan Pelatihan Pembuatan Turunan Hasil Olahan dari Produk Lebah Kelulut pada Kelompok Peternak Lebah di Samarinda. PengabdianMu J Ilmu Pengabdian Masyarakat. 2023;8(1):21–6. DOI: 10.33084/pengabdianmu.v8i1.4200
26. Alsaedy NS, Almilly RF, Al-Shannag M. Testing of a Potentially Used Antiseptic Consists of Povidon Iodine, Hydrogen Peroxide and Aloe Vera. Iraqi J Chem Pet Eng. 2023;24(1):105–12. DOI: 10.31699/IJCPE.2023.1.12
27. Bouchama C, Zinedine A, Rocha JM, Chadli N, El Ghadraoui L, Chabir R, et al. Effect of phenolic compounds extracted from turmeric (Curcuma longa L.) and ginger (Zingiber officinale) on cutaneous wound healing in Wistar rats. Cosmetics. 2023;10(5):137. DOI: 10.3390/cosmetics10050137
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2. Gonzales ACdO, Costa TF, Andrade ZdA, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016;91(5):614-20. DOI: 10.1590/abd1806-4841.20164741; PMCID: PMC5087220; PMID: 27828635
3. Tottoli EM, Dorati R, Genta I, Chiesa E, Pisani S, Conti B. Skin wound healing process and new emerging technologies for skin wound care and regeneration. Pharmaceutics. 2020;12(8):735. DOI: 10.3390/pharmaceutics12080735; PMCID: PMC7463929; PMID: 32764269
4. Zhou S, Xie M, Su J, Cai B, Li J, Zhang K. New insights into balancing wound healing and scarless skin repair. J Tissue Eng. 2023;14:20417314231185850. DOI: 10.1177/20417314231185848; PMCID: PMC10388637; PMID: 37529248
5. Demidova-Rice TN, Hamblin MR, Herman IM. Acute and impaired wound healing: pathophysiology and current methods for drug delivery, part 1: normal and chronic wounds: biology, causes, and approaches to care. Adv Skin Wound Care. 2012;25(7):304-14. DOI: 10.1097/01.asw.0000416006.55218.d0; PMCID: PMC3428147; PMID: 22713781
6. Comino-Sanz IM, López-Franco MD, Castro B, Pancorbo-Hidalgo PL. The Role of Antioxidants on Wound Healing: A Review of the Current Evidence.J Clin Med. 2021;10(16):3558. DOI: 10.3390/jcm10163558; PMCID: PMC8397081; PMID: 34441854
7. Criollo-Mendoza MS, Contreras-Angulo LA, Leyva-López N, Gutiérrez-Grijalva EP, Jiménez-Ortega LA, Heredia JB. Wound Healing Properties of Natural Products: Mechanisms of Action. Molecules. 2023;28(2):598. DOI: 10.3390/molecules28020598; PMCID: PMC9867334; PMID: 36677659
8. Singh LR, Singh K. Holistic health potential of indian pennywort (centella asiatica (l.) urban). Int J Dev Res. 2017;7(12):17725–8.
9. Jantan I, Saputri FC, Qaisar MN, Buang F. Correlation between Chemical Composition of Curcuma domestica and Curcuma xanthorrhiza and Their Antioxidant Effect on Human Low-Density Lipoprotein Oxidation. Evid Based Complement Altern Med. 2012;2012:438356. DOI: 10.1155/2012/438356; PMCID: PMC3519093; PMID: 23243446
10. Bigliardi PL, Alsagoff SAL, El-Kafrawi HY, Pyon JK, Wa CTC, Villa MA. Povidone iodine in wound healing: A review of current concepts and practices. Int J Surg. 2017;44:260–8. DOI: 10.1016/j.ijsu.2017.06.073; PMID: 28648795
11. Batistuta MA, Aulia A, Kustiawan PM. Review : Potensi Aktivitas Anti Virus dari Produk Alami Lebah Kelulut. J Farmasi Udayana. 2021;10(2):144-8. DOI: 10.24843/JFU.2021.v10.i02.p06
12. Bian Y, Zhang Y, Zhou Y, Li GH, Feng XS. Progress in the pretreatment and analysis of flavonoids: an update since 2013. Sep Purif Rev. 2022;51(1):11–37. DOI: 10.1080/15422119.2020.1801469
13. Putri HA, Mulyanti D. Karakterisasi Simplisia dan Ekstrak Etanol Daun Pegagan (Centella asiatica (L.) Urban). J Riset Farmasi. 2023;3(1):43-8. DOI: 10.29313/jrf.v3i1.3120
14. Asfi D. Uji Efektifitas Sediaan Gel Ekstrak Etanol Herba Bandotan (Ageratum conyzoides L) Terhadap Luka Bakar Pada Hewan Uji Kelinci (Oryctolagus cuniculus). J Kesehatan Yamasi Makassar. 2019;3(1):1-4.
15. Effendi F, Halimatussa’diyah, Helmina S. Efektivitas Penyembuhan Luka Terbuka Pada Kelinci Dari Formulasi Salep Ekstrak Etanol Daun Babandotan (Ageratum conyzoides L.). J Farmamedika Pharmamedica J. 2017;2(1):7–16. DOI: 10.47219/ath.v2i1.26
16. Diniz LRL, Calado LL, Duarte ABS, de Sousa DP. Centella asiatica and Its Metabolite Asiatic Acid: Wound Healing Effects and Therapeutic Potential. Metabolites. 2023;13(2):276. DOI: 10.3390/metabo13020276; PMCID: PMC9966672; PMID: 36837896
17. Santoso TA, Diniatik, Kusuma AM. Efek Imunostimulator Ekstrak Etanol Daun Katuk (Sauropus androgynus L Merr) terhadap Aktivitas Fagositosis Makrofag. Pharmacy J Farmasi Indones Pharm J Indones. 2013;10(1):63-70. DOI: 10.30595/pji.v10i1.786
18. Mishra B. Assessment of plant diversity: a medicinal, conservational and environmental study. Asian J Biol. 2023;18(1):39–57. DOI: 10.9734/ajob/2023/v18i1336
19. Nakatani N. Phenolic antioxidants from herbs and spices. Biofactors. 2000;13(1-4):141–6. DOI: 10.1002/biof.5520130123; PMID: 11237173
20. Min DH, Yu YB, Kim TH, Kim H, Lee S. Pharmacological effects of pentacyclic triterpenoids isolated from Centella asiatica. Hortic Environ Biotechnol. 2024;65(2):189–97. DOI: 10.1007/s13580-023-00561-8
21. Swain SS, Sahoo G, Mahapatra PK, Panda SK. Disease burden and current therapeutical status of leprosy with special emphasis on phytochemicals. Curr Top Med Chem. 2022;22(19):1611–25. DOI: 10.2174/1568026621666210909162435
22. Taghizadeh M, Jalili S. Phytochemical content, antioxidant properties, and antibacterial activities of Centella asiatica L. Nat Prod Res. 2024;38(20):3693-8. DOI: 10.1080/14786419.2023.2258439; PMID: 37708329
23. Fahryl N, Carolia N. Kunyit (Curcuma domestica Val) sebagai Terapi artritis gout. Majority Med J Lampung Univ. 2019;8(1):251–5.
24. Esa NEF, Ansari MNM, Razak SIA, Ismail NI, Jusoh N, Zawawi NA, et al. A Review on Recent Progress of Stingless Bee Honey and Its Hydrogel-Based Compound for Wound Care Management. Molecules. 2022;27(10):3080. DOI: 10.3390/molecules27103080; PMCID: PMC9145090; PMID: 35630557
25. Kustiawan PM, Hanifa DNC, Nugraha ASD, Suwandi A, Monica A, Agustinur A. Edukasi dan Pelatihan Pembuatan Turunan Hasil Olahan dari Produk Lebah Kelulut pada Kelompok Peternak Lebah di Samarinda. PengabdianMu J Ilmu Pengabdian Masyarakat. 2023;8(1):21–6. DOI: 10.33084/pengabdianmu.v8i1.4200
26. Alsaedy NS, Almilly RF, Al-Shannag M. Testing of a Potentially Used Antiseptic Consists of Povidon Iodine, Hydrogen Peroxide and Aloe Vera. Iraqi J Chem Pet Eng. 2023;24(1):105–12. DOI: 10.31699/IJCPE.2023.1.12
27. Bouchama C, Zinedine A, Rocha JM, Chadli N, El Ghadraoui L, Chabir R, et al. Effect of phenolic compounds extracted from turmeric (Curcuma longa L.) and ginger (Zingiber officinale) on cutaneous wound healing in Wistar rats. Cosmetics. 2023;10(5):137. DOI: 10.3390/cosmetics10050137
Authors
1.
Diniatik D, Kustiawan PM, Suryadini H. Standardized Herbal Extract for Wound Healing: A Comparative Study of Centella asiatica, Curcuma domestica, and Heterotrigona itama Honey Combination in Rabbits. Borneo J Pharm [Internet]. 2024Nov.30 [cited 2025Mar.11];7(4):404-11. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/5803
Copyright (c) 2024 Diniatik Diniatik, Paula Mariana Kustiawan, Halida Suryadini
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