Antioxidant Activity of Edible Bird’s Nest (Aerodramus fuciphagus) from Central Borneo, Indonesia
DOI:
https://doi.org/10.33084/bjop.v8i1.6595Keywords:
DPPH, CUPRAC, FRAP, NO MethodAbstract
Edible bird's nests (EBNs) from Aerodramus fuciphagus have been traditionally used in Asia for their various health benefits, including antioxidant properties. Previous studies have reported antioxidant activity in EBN water extracts using methods like ABTS and ORAC. However, the antioxidant activity can vary significantly depending on factors such as the source of EBN, extraction methods, and the specific assay used. This study aimed to comprehensively evaluate the antioxidant potential of EBN water extract using a range of in vitro assays. The antioxidant activity was assessed using four different methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, nitric oxide (NO) radical scavenging, ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC). Results demonstrated that the EBN water extract exhibited moderate NO radical scavenging activity with an IC50 value of 116.86 μg/mL. While weak activity was observed in the DPPH assay, the FRAP and CUPRAC assays showed promising antioxidant potential with an ascorbic acid equivalent (AAE) of 4.567±0.30 and 3.487±0.095 μg AAE/mL, respectively. These findings suggest that the antioxidant activity of EBN water extract may be primarily attributed to its protein content. However, further investigations are warranted to elucidate the specific mechanisms of action and isolate the bioactive compounds responsible for the observed antioxidant effects. These findings have implications for the development of EBN-based functional foods, nutraceuticals, and cosmeceuticals with enhanced antioxidant properties.
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Lee TH, Wani WA, Lee CH, Cheng KK, Shreaz S, Wong S, et al. Edible Bird's Nest: The Functional Values of the Prized Animal-Based Bioproduct From Southeast Asia-A Review. Front Pharmacol. 2021;12:626233. DOI: 10.3389/fphar.2021.626233; PMCID: PMC8089372; PMID: 33953670
Thorburn C. The Edible Birds' Nest Boom in Indonesia and South-east Asia. Food Cult Soc. 2014;17(4):535-53. DOI: 10.2752/175174414X14006746101439
Yacoob MR, Khairy WIW, Munirah AR, Nabilah JNF, Zulhazman H. Suitable habitat and environmental conditions for successful edible bird nest swiftlet houses. AIP Conf Proc. 2021;2347:020197. DOI: 10.1063/5.0054174
Ito Y, Matsumoto K, Usup A, Yamamoto Y. A sustainable way of agricultural livelihood: edible bird’s nests in Indonesia. Ecosyst Health Sust. 2021;7(1):1960200. DOI: 10.1080/20964129.2021.1960200
Sandi DAD, Musfirah Y. Wound Healing Effects of Edible Bird’s Nests Oinment (Aerodramus fuciphagus) in Alloxan-Induced Male Rats. Majalah Obat Tradisional. 2019;24(1):33–9. DOI: 10.22146/mot.39072
Sandi DAD, Rahmatullah SW. Pengujian Efek Tonikum Sarang Burung Walet Putih (Aerodramus fuchipagus) pada Mencit Putih Jantan dengan Metode Ketahanan Lama Waktu Berenang. J Pharmascience. 2016;3(2):29–35. DOI: 10.20527/jps.v3i2.5735
Sandi DAD, Susiani EF. Formulation of edible bird’s nest (Aerodramus fuciphagus) from Central Kalimantan as skin whitening and moisturizing cream. J Pharm Bioallied Sci. 2021;13(1):39–45. DOI: 10.4103/jpbs.jpbs_276_19; PMCID: PMC8142912; PMID: 34084047
Sandi DAD, Rahmatullah SW. Stimulant Effect of Edible Bird’s Nest (Aerodramus fuchipagus) from Borneo on White Mice. Res J Pharm Biol Chem Sci. 2018;9:927–30.
Elfita L, Wientarsih I, Sajuthi D, Bachtiar I, Darusman HS. The diversity in nutritional profile of farmed edible bird’s nests from several regions in Indonesia. Biodiversitas. 2020;21(6):2362–8. DOI: 10.13057/biodiv/d210604
Chantakun K, Kishimura H, Kumagai Y, Benjakul S. Physicochemical properties of house and cave edible bird’s nest from Southern Thailand. ScienceAsia. 2022;48(2):136–43. DOI: 10.2306/scienceasia1513-1874.2022.017
Helmi, Subekti DT, Mranata B, Sudarnika E, Lukman DW, Wibawan IWT. Protein Profile of Edible Bird’s Nest Origin Kalimantan AndJava Islands Indonesia. IOSR J Agric Vet Sci. 2018;11(5):69–73. DOI: 10.9790/2380-1105026973
Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition. 2002;18(10):872–9. DOI: 10.1016/s0899-9007(02)00916-4; PMID: 12361782
Tung CH, Pan JQ, Chang HM, Chou SS. Authentic determination of bird’s nests by saccharides profile. J Food Drug Anal. 2008;16(4):86–91. DOI: 10.38212/2224-6614.2339
Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Razis AFA, Modu B, et al. Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Front Chem. 2023;11:1158198. DOI: 10.3389/fchem.2023.1158198; PMCID: PMC10206224; PMID: 37234200
Yida Z, Imam MU, Ismail M. In Vitro Bioaccessibility and Antioxidant Properties of Edible Bird’s Nest Following Simulated Human Gastro-Intestinal Digestion. BMC Complement Altern Med. 2014;14:468. DOI: 10.1186/1472-6882-14-468; PMCID: PMC4289220; PMID: 25475744
Maesaroh K, Kurnia D, Al Anshori J. Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC terhadap Asam Askorbat, Asam Galat dan Kuersetin. J Chim Nat Acta. 2018 Aug 26;6(2):93-100. DOI: 10.24198/cna.v6.n2.19049
Zamzani I, Triadisti N. Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method. J Profesi Medika J Kedokteran Kesehatan. 2021;15(1):60-8. DOI: 10.33533/jpm.v15i1.2820
Gulcin I, Alwasel SH. DPPH Radical Scavenging Assay. Processes. 2023;11(8):2248. DOI: 10.3390/pr11082248
Sadeer NB, Montesano D, Albrizio S, Zengin G, Mahomoodally MF. The Versatility of Antioxidant Assays in Food Science and Safety-Chemistry, Applications, Strengths, and Limitations. Antioxidants. 2020;9(8):709. DOI: 10.3390/antiox9080709; PMCID: PMC7464350; PMID: 32764410
Nwachukwu ID, Sarteshnizi RA, Udenigwe CC, Aluko RE. A Concise Review of Current In Vitro Chemical and Cell-Based Antioxidant Assay Methods. Molecules. 2021;26(16):4865. DOI: 10.3390/molecules26164865; PMCID: PMC8400447; PMID: 34443459
Gülçin I. Fe(3+)-Fe(2+) transformation method: an important antioxidant assay. Methods Mol Biol. 2015;1208:233-46. DOI: 10.1007/978-1-4939-1441-8_17; PMID: 25323511
Özyürek M, Güçlü K, Tütem E, Başkan KS, Erçağ E, Çelik SE, et al. A comprehensive review of CUPRAC methodology. Anal Methods. 2011;3(11):2439-53. DOI: 10.1039/C1AY05320E
Sukhtam T, Jones A, Soliven A, Dennis GR, Shalliker RA. A comparison of the performance of the cupric reducing antioxidant potential assay and the ferric reducing antioxidant power assay for the analysis of antioxidants using reaction flow chromatography. Microchem J. 2019;149:104046. DOI: 10.1016/j.microc.2019.104046
Klompong V, Benjakul S, Yachai M, Visessanguan W, Shahidi F, Hayes KD. Amino acid composition and antioxidative peptides from protein hydrolysates of yellow stripe Trevally (Selaroides leptolepis). J Food Sci. 2009;74(2):C126-33. DOI: 10.1111/j.1750-3841.2009.01047.x; PMID: 19323726
Varki A, Cummings RD, Esko JD, Freeze HH, Stanley P, Bertozzi CR, et al. Essential of Glycobiology. 2nd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009. PMID: 20301239; NBKID: NBK1908
Rifqi A. Perbandingan metode ekstraksi dan uji aktivitas antioksidan ekstrak sarang burung walet (collocalia i.-uciphaga) dengan metode DPPH (2,2-Difenil-l-l- Pikrihidrazil) [bachelor’s thesis]. Jakarta: Universitas Islam Negeri Syarif Hidayatullah; 2017. Available from: http://repository.uinjkt.ac.id/dspace/handle/123456789/37323
Quek MC, Chin NL, Yusof YA, Law CL, Tan SW. Characterization of edible bird’s nest of different production, species and geographical origins using nutritional composition, physicochemical properties and antioxidant activities. Food Res Int. 2018;109:35–43. DOI: 10.1016/j.foodres.2018.03.078; PMID: 29803459
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