Analisis Hasil Deteksi SARS-COV2 Menggunakan Rapid Tes Antigen di Laboratorium Mikrobiologi Klinik RSUD dr. Doris Sylvanus Palangkaraya
Analysis of SARS-COV2 Detection Using Antigen Rapid Tes in the Laboratory Clinical Microbiology RSUD dr. Doris Sylvanus Palangkaraya
DOI:
https://doi.org/10.33084/jsm.v9i2.4417Keywords:
Rapid Test Antigen, Spesifisitas, Sensitivitas, Uji Diagnostik, COVID-19Abstract
The antigen rapid diagnostic test is used for screening COVID-19 patients and is easy to do anywhere and saves time. The sensitivity of various brands of rapid antigen test kits can vary, so medical personnel need to pay close attention to them. Testing for the SARS-CoV2 antigen kit needs to be done diagnostically. This study aims to determine the specificity, sensitivity, and accuracy of the Haelgen antigen rapid test through diagnostic tests. The study design was cross-sectional, with a total sample of 60 people who took swabs from the nasopharynx and oropharynx. The sample is placed in the viral transport medium (VTM). Samples were examined by RT-PCR and, at the same time, examined using an antigen rapid test. The results of the analysis were carried out by calculating the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of the SARS-Cov2 antigen test compared to standard RT-PCR using a diagnostic test formula. The most common characteristics of COVID-19 patients were female and 20–29 years old (28.3%). The results of the Haelgen rapid antigen diagnostic test showed a sensitivity of 92.31%, a specificity of 100%, a positive predictive value of 100%, a negative predictive value of 87.5%, and an accuracy of 95%. The sensitivity, specificity, and accuracy of the Haelgen rapid antigen are suitable for testing for COVID-19.
Downloads
References
CDC. (2020). Coronavirus Disease 2019 (COVID-19): Interim Guidelines for COVID-19 Antibody Testing in Clinical and Public Health Settings, US Department of Health and Human Services, diakses 16 September 2020: https://www.cdc.gov/coronavirus/2019-ncov/lab/resources/antibody-tests-guidelines.htm.
Chaimayo C, Kaewnaphan B, Tanlieng N, et al. (2020). Rapid Sars-CoV-2 antigen detection assay in comparison with real-time RT-PCR assay for laboratory diagnosis of COVID-19 in Thailand. Virol J. 2020;17(1):177.
Hirotsu Y, Maejima M, Shibusawa M, et al. (2020). Comparison of automated Sars-CoV-2 antigen test for COVID-19 infection with quantitative RT-PCR using 313 nasopharyngeal swabs, including from seven serially followed patients. Int J Infect Dis.2020; 99:397-402. doi:10.1016/j.ijid.2020.08.029
Hartantoro D, Nuryati A, Nuryani S. (2021). Uji diagnostik uji antigen Sars-CoV-2 pada pemeriksaan laboratorium Covid-19: Studi kasus di RS Beriman Kota Balikpapan, Indonesia. Puinovakesmas. 2021;2(2):55–61.
Kementerian Kesehatan Republik Indonesia. (2020). Pedoman Kesiapsiagaan Menghadapi Infeksi Novel Coronavirus (2019-nCov). Direktorat Jenderal Pencegah dan Pengendali Penyakit. Published online 2020:0-74.
Kurniawan, E., Raveinal, Fauzar dan Arsyad Z. (2017). Nilai Diagnostik Metode Real Tme PCR GeneXpert Pada Tuberkulosis Paru BTA Negatif. 12 Desember 2017, Padang.
Mak GC, Cheng PK, Lau SS, et al. (2020). Evaluation of rapid antigen test for detection of Sars-CoV-2 virus. Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology. 2020;129:104500-.
Multi Media Center Provinsi Kalimantan Tengah. (2022). Kasus Harian Covid-19 di Kalteng. Palangka Raya
Nagura-Ikeda M, Imai K, Tabata S, et al. (2020). Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription-Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19. J Clin Microbiol. 2020;58(9):e01438-20. doi:10.1128/JCM.01438-20
Porte L, Legarraga P, Vollrath V, et al. (2020). Evaluation of a novel antigen-based rapid detection test for the diagnosis of Sars-CoV-2 in respiratory samples. Int J Infect Dis. 2020;99:328-333. doi:10.1016/j.ijid.2020.05.098
Qin C, Zhou L, Hu Z, et al. (2020). Dysregulation of Immune Response in Patients With Coronavirus 2019 (COVID-19) in Wuhan, China. Clin Infect Dis. 2020;71(15):762-768. doi:10.1093/cid/ciaa248
Rahmadhaniati, Dinna Rakhmina, Tini Elyn Herlina. (2022). Perbandingan Hasil Diagnosis Laboratorium pada COVID-19 menggunakan Metode Real Time-Polymerase Chain Reaction (RT-PCR) dan Metode Rapid Test Antigen. Poltekkes Kemenkes Banjarmasin. Jurnal kesehatan. 2022;15(1).
Scohy A, Anantharajah A, Bodéus M, Kabamba-Mukadi B, Verroken A, Rodriguez-Villalobos H. (2020). Low performance of rapid antigen detection test as frontline testing for COVID-19 diagnosis. J Clin Virol. 2020;129:104455. doi:10.1016/j.jcv.2020.104455
WHO. Deteksi antigen dalam diagnosis infeksi Sars-CoV-2 menggunakan imunoasai cepat. 2020. https://www.who.int/docs/ default-source/searo /indonesia/covid19/deteks i-antigen-dalam-diagnosis- infeksi-Sars-CoV-2-menggunakan -imunoasai-cepat.pdf? sfvrsn=222f2be3_2
World Health Organization. Laboratory testing of 2019 novel coronavirus (2019-nCoV) in suspected human cases: interim guidance, 17 January 2020 [Internet]. World Health Organization; 2020. Available from: https://apps.who.int/iris/ handle/10665/330676
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Silvani Permatasari, Florence Felicia, Ni Putu W.S.W, Misbah Misbah, Arnino A, Dede Z. A, Yanuardi Ikhsan, Rini Fitri R, Chandra Chandra, Febrianto E. P
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
All rights reserved. This publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording.
