Antibiotics Resistance Pattern of Coliform Bacteria Isolated from Slaughterhouse Wastewater in Jega Town, Kebbi State, Nigeria
Article Sidebar
Submitted
12 July 2020
Published
31 August 2020
Keywords:
-
Antibiotics, Coliform bacteria, Wastewater, Jega, Slaughterhouse, Resistant
Abstract
Wastewater is an essential reservoir of pathogenic bacteria, which include resistant strains. This study determined the antibiotics resistance pattern of Coliform bacteria isolated from slaughterhouse wastewater in Jega local government. Five different samples of wastewater from different locations of a slaughterhouse were collected using standard sample collection techniques. Coliform bacteria were isolated using the standard microbiological method. The total bacterial count was determined using plate count agar. Suspected coliforms were identified based on morphological and biochemical tests. Antimicrobial susceptibility testing was carried out using the standard disc diffusion method. The highest bacterial count (8.4x102 CFU/ml) was observed from the wastewater collection point, and the lowest bacterial (1.2x102 CFU/ml) count from the slaughtering point. Three Gram-negative bacteria, Escherichia coli, Pseudomonas aeruginosa, and Enterobacter aerogenes, were identified. Escherichia coli was the most frequently isolated in slaughterhouse wastewater 39 (45.8%). Among the antibiotics tested against isolated bacteria, Septrin was the most resistant antibiotics recorded against E. coli and E. aerogenes with 84.61% and 88.89% resistant, respectively. Pseudomonas aeruginosa was found to show higher resistance to Chloramphenicol and Septrin with 84.21% resistance each. The occurrence of antibiotic-resistant bacteria from slaughterhouse wastewater showed the risks associated with antimicrobial drug resistance transferred from food-producing animals to humans. Management concerns, such as local government health officers and community development officers, should increase the sensitization of slaughterhouse workers by organizing conferences or conducting radio talk to educates these slaughterhouse workers on the treatment of slaughterhouse wastewater and health risk associated with antimicrobial-drug resistance transferred from animals to humans.
Full text article
Generated from XML file
References
Adebowale, O.O., Adedamola, J., Olanike, A., & Eniola, K. (2016). Potential Bacterial Zoonotic Pathogens Isolated from a Major Abattoir and its Receiving Surface Water in Abeokuta, Nigeria. Alexandria Journal of Veterinary Sciences, 50(1), 94-98. doi:10.5455/ajvs.222912
Ajibola, O., Omoleke, S.A., & Omisakin, O.A. (2018). Current status of cerebrospinal meningitis and impact of the 2015 meningococcal C vaccination in Kebbi, Northwest Nigeria. Vaccine, 36(11), 1423-1428. doi:10.1016/j.vaccine.2018.01.084
Aslam, B., Wang, W., Arshad, M.I., Khurshid, M., Muzammil, S., Rasool, M.H., Nisar, M.A., Alvi, R.F., Aslam, M.A., Qamar, M.U., Salamat, M.K.F., & Baloch, Z. (2018). Antibiotic resistance: a rundown of a global crisis. Infection and Drug Resistance, 11, 1645-1658. doi:10.2147/IDR.S173867
Atuanya, E.I., Nwogu, N.A., & Orah, C.U. (2018). Antibiotic Resistance and Plasmid Profiles of Bacteria Isolated from Abattoir Effluents around Ikpoba River in Benin City, Nigeria. Journal of Applied Sciences and Environmental Management, 22(11), 1749-1755. doi:10.4314/jasem.v22i11.7
Ayogu, T.E., Orji, J.O., Nwojiji, E.C., Umezurike, R.C., & Ibiam, U.U. (2018). Antibiotic Susceptibility Pattern of Salmonella and Pseudomonas Species Isolated from Meat Market and Ogoja Road Abattoir Effluents in Abakaliki Metropolis. World Journal of Medical Sciences, 15(1), 34-47. doi:10.5829/idosi.wjms.2018.34.47
Bashir, I., Adam, A.S., Yahaya, H.S., Makeri, D., Ntulume, I., Aliero, A.A., & Afolabi, R.O. (2018). Assessment of bacteriological quality of borehole water in Wamakko local government, Sokoto state, Nigeria. Novel Research in Microbiology Journal, 2(6), 175-184. doi:10.21608/NRMJ.2018.22710
Cheesbrough, M. (2006). District Laboratory Practice in Tropical Countries. 2nd Edition. Part 2. London, UK: Cambridge University Press. p.425.
Clinical and Laboratory Standards Institute. 2019. Performance standards for antimicrobial susceptibility testing. 29th edition. CLSI Supplement M100. Wayne, Pennsylvania, US: Clinical and Laboratory Standards Institute. p.26.
Egesi, C.O., Ezebuiro, V., Ekeleme, A.C., & Obiukwu, C.E. (2019). Compliance Monitoring of Microbiological and Physicochemical Parameters of Abattoirs’ Effluents Discharged into Water Bodies in Owerri, Nigeria. Microbiology Research Journal International, 28(6), 1-16. doi:10.9734/mrji/2019/v28i630147
Egwuenu, A., Obasanya, J., Okeke, I., Aboderin, O., Olayinka, A., Kwange, D., Ogunniyi, A., Mbadiwe, E., Omoniyei, L., Omotayo, H., Niyang, M., Abba, F., Kudla, F., TWG, A., & Ihekweazu, C. (2018). Antimicrobial use and resistance in Nigeria: situation analysis and recommendations, 2017. Pan African Medical Journal - Conference Proceedings, 8(8), 2. doi:10.11604/pamj.cp.2018.8.2.701
Flores-Mireles, A.L., Walker, J.N., Caparon, M., & Hultgren, S.J. (2015). Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nature Reviews Microbiology, 13(5), 269-284. doi:10.1038/nrmicro3432
Hiroi, M., Kawamori, F., Harada, T., Sano, Y., Miwa, N., Sugiyama, K., Hara-Kudo, Y., & Masuda, T. (2012). Antibiotic resistance in bacterial pathogens from retail raw meats and food-producing animals in Japan. Journal of Food Protection, 75(10), 1774-1782. doi:10.4315/0362-028x.jfp-11-479
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Stanley, J.T., & William, S.T. (1994) Bergey’s Manual of Determinative Bacteriology. Baltimore, US: Lippincott Williams & Wilkins. p.566.
Igbinosa, E.O., Odjadjare, E.E., Igbinosa, I.H., Orhue, P.O., Omoigberale, M.N.O., & Amhanre, N.I. (2012). Antibiotic Synergy Interaction against Multidrug-Resistant Pseudomonas aeruginosa Isolated from an Abattoir Effluent Environment. The Scientific World Journal, 2012, 308034. doi:10.1100/2012/308034
Jega, B.G., Adebisi, O.O., & Manga, S.S. (2018). Antibiotic Susceptibility of Bacteria Isolated from Abattoir Effluent-Impacted Tagangu River, Aliero, Kebbi State, North-Western Nigeria. South Asian Journal of Research in Microbiology, 2(4), 1-8. doi:10.9734/sajrm/2018/v2i430072
Johnson, O.E. & Etokidem, A.J. (2019). Occupational Hazards and Health Problems among Butchers in Uyo, Nigeria. Nigerian Medical Journal, 60(3), 106-112. doi:10.4103/nmj.NMJ_57_19
Kundu, P., Debsarkar, A., & Mukherjee, S. (2013). Treatment of Slaughter House Wastewater in a Sequencing Batch Reactor: Performance Evaluation and Biodegradation Kinetics. BioMed Research International, 2013, 134872. doi:10.1155/2013/134872
Manyi-Loh, C., Mamphweli, S., Meyer, E., & Okoh, A. (2018). Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. Molecules, 23(4), 795. doi:10.3390/molecules23040795
Nafarnda, W.D., Ajayi, I.E., Shawulu, J.C., Kawe, M.S., Omeiza, G.K., Sani, N.A., Tenuche, O.Z., Dantong, D.D., & Tags, S.Z. (2012). Bacteriological Quality of Abattoir Effluents Discharged into Water Bodies in Abuja, Nigeria. International Scholarly Research Notices, 2012, 515689. doi:10.5402/2012/515689
Ogbomida, E.T., Kubeyinje, B., & Ezemonye, L.I. (2016). Evaluation of bacterial profile and biodegradation potential of abattoir wastewater. African Journal of Environmental Science and Technology, 10(2), 50-57. doi:10.5897/AJEST2015.1945
de Oliveira, K.M.P., Júlio, P.D.S., & Grisolia, A.B. (2013). Antimicrobial susceptibility profile of Pseudomonas spp. isolated from a swine slaughterhouse in Dourados, Mato Grosso do Sul State, Brazil. Revista Argentina de Microbiología, 45(1), 57-60.
Rabiu, A.G. & Falodun, O.I. (2017). Multi-drug Resistant Pseudomonas Species Isolated from the Wastewater of an Abattoir in Ibadan, Nigeria. Journal of Applied Life Sciences International, 13(1), 1-9. doi:10.9734/JALSI/2017/34522
Santajit, S. & Indrawattana, N. (2016). Mechanisms of Antimicrobial Resistance in ESKAPE Pathogens. BioMed Research International, 2016, 2475067. doi:10.1155/2016/2475067
Savin, M., Bierbaum, G., Hammerl, J.A. Heinemann, C., Parcina, M., Sib, E., Voigt, A., & Kreyenschmidt, J. (2020). Antibiotic-resistant bacteria and antimicrobial residues in wastewater and process water from German pig slaughterhouses and their receiving municipal wastewater treatment plants. Science of The Total Environment, 727, 138788. doi:10.1016/j.scitotenv.2020.138788
Shrivastava, S.R., Shrivastava, P.S., & Ramasamy, J. (2018). World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. Journal of Medical Society, 32(1), 76-77. doi:10.4103/jms.jms_25_17
Tesfaye, H., Alemayehu, H., Desta, A.F., & Eguale, T. (2019). Antimicrobial susceptibility profile of selected Enterobacteriaceae in wastewater samples from health facilities, abattoir, downstream rivers and a WWTP in Addis Ababa, Ethiopia. Antimicrobial Resistance and Infection Control, 8(1), 134. doi:10.1186/s13756-019-0588-1
Tran, C.S., Rangel, S.M., Almbad, H., Kierbel, A., Givskov, M., Tolker-Nielsen, T., Hauser, A.R., & Engel, J.N. (2014). The Pseudomonas aeruginosa type III translocon is required for biofilm formation at the epithelial barrier. PLoS Pathogens, 10(11), e1004479. doi:10.1371/journal.ppat.1004479
World Health Organization. (2017). WHO publishes list of bacteria for which new antibiotics are urgently needed. https://www.who.int/news-room/detail/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed
Yayan, J., Ghebremedhin, B., & Rasche, K. (2015). Antibiotic Resistance of Pseudomonas aeruginosa in Pneumonia at a Single University Hospital Center in Germany over a 10-Year Period. PLoS One, 10(10), e0139836. doi:10.1371/journal.pone.0139836
Ajibola, O., Omoleke, S.A., & Omisakin, O.A. (2018). Current status of cerebrospinal meningitis and impact of the 2015 meningococcal C vaccination in Kebbi, Northwest Nigeria. Vaccine, 36(11), 1423-1428. doi:10.1016/j.vaccine.2018.01.084
Aslam, B., Wang, W., Arshad, M.I., Khurshid, M., Muzammil, S., Rasool, M.H., Nisar, M.A., Alvi, R.F., Aslam, M.A., Qamar, M.U., Salamat, M.K.F., & Baloch, Z. (2018). Antibiotic resistance: a rundown of a global crisis. Infection and Drug Resistance, 11, 1645-1658. doi:10.2147/IDR.S173867
Atuanya, E.I., Nwogu, N.A., & Orah, C.U. (2018). Antibiotic Resistance and Plasmid Profiles of Bacteria Isolated from Abattoir Effluents around Ikpoba River in Benin City, Nigeria. Journal of Applied Sciences and Environmental Management, 22(11), 1749-1755. doi:10.4314/jasem.v22i11.7
Ayogu, T.E., Orji, J.O., Nwojiji, E.C., Umezurike, R.C., & Ibiam, U.U. (2018). Antibiotic Susceptibility Pattern of Salmonella and Pseudomonas Species Isolated from Meat Market and Ogoja Road Abattoir Effluents in Abakaliki Metropolis. World Journal of Medical Sciences, 15(1), 34-47. doi:10.5829/idosi.wjms.2018.34.47
Bashir, I., Adam, A.S., Yahaya, H.S., Makeri, D., Ntulume, I., Aliero, A.A., & Afolabi, R.O. (2018). Assessment of bacteriological quality of borehole water in Wamakko local government, Sokoto state, Nigeria. Novel Research in Microbiology Journal, 2(6), 175-184. doi:10.21608/NRMJ.2018.22710
Cheesbrough, M. (2006). District Laboratory Practice in Tropical Countries. 2nd Edition. Part 2. London, UK: Cambridge University Press. p.425.
Clinical and Laboratory Standards Institute. 2019. Performance standards for antimicrobial susceptibility testing. 29th edition. CLSI Supplement M100. Wayne, Pennsylvania, US: Clinical and Laboratory Standards Institute. p.26.
Egesi, C.O., Ezebuiro, V., Ekeleme, A.C., & Obiukwu, C.E. (2019). Compliance Monitoring of Microbiological and Physicochemical Parameters of Abattoirs’ Effluents Discharged into Water Bodies in Owerri, Nigeria. Microbiology Research Journal International, 28(6), 1-16. doi:10.9734/mrji/2019/v28i630147
Egwuenu, A., Obasanya, J., Okeke, I., Aboderin, O., Olayinka, A., Kwange, D., Ogunniyi, A., Mbadiwe, E., Omoniyei, L., Omotayo, H., Niyang, M., Abba, F., Kudla, F., TWG, A., & Ihekweazu, C. (2018). Antimicrobial use and resistance in Nigeria: situation analysis and recommendations, 2017. Pan African Medical Journal - Conference Proceedings, 8(8), 2. doi:10.11604/pamj.cp.2018.8.2.701
Flores-Mireles, A.L., Walker, J.N., Caparon, M., & Hultgren, S.J. (2015). Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nature Reviews Microbiology, 13(5), 269-284. doi:10.1038/nrmicro3432
Hiroi, M., Kawamori, F., Harada, T., Sano, Y., Miwa, N., Sugiyama, K., Hara-Kudo, Y., & Masuda, T. (2012). Antibiotic resistance in bacterial pathogens from retail raw meats and food-producing animals in Japan. Journal of Food Protection, 75(10), 1774-1782. doi:10.4315/0362-028x.jfp-11-479
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Stanley, J.T., & William, S.T. (1994) Bergey’s Manual of Determinative Bacteriology. Baltimore, US: Lippincott Williams & Wilkins. p.566.
Igbinosa, E.O., Odjadjare, E.E., Igbinosa, I.H., Orhue, P.O., Omoigberale, M.N.O., & Amhanre, N.I. (2012). Antibiotic Synergy Interaction against Multidrug-Resistant Pseudomonas aeruginosa Isolated from an Abattoir Effluent Environment. The Scientific World Journal, 2012, 308034. doi:10.1100/2012/308034
Jega, B.G., Adebisi, O.O., & Manga, S.S. (2018). Antibiotic Susceptibility of Bacteria Isolated from Abattoir Effluent-Impacted Tagangu River, Aliero, Kebbi State, North-Western Nigeria. South Asian Journal of Research in Microbiology, 2(4), 1-8. doi:10.9734/sajrm/2018/v2i430072
Johnson, O.E. & Etokidem, A.J. (2019). Occupational Hazards and Health Problems among Butchers in Uyo, Nigeria. Nigerian Medical Journal, 60(3), 106-112. doi:10.4103/nmj.NMJ_57_19
Kundu, P., Debsarkar, A., & Mukherjee, S. (2013). Treatment of Slaughter House Wastewater in a Sequencing Batch Reactor: Performance Evaluation and Biodegradation Kinetics. BioMed Research International, 2013, 134872. doi:10.1155/2013/134872
Manyi-Loh, C., Mamphweli, S., Meyer, E., & Okoh, A. (2018). Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. Molecules, 23(4), 795. doi:10.3390/molecules23040795
Nafarnda, W.D., Ajayi, I.E., Shawulu, J.C., Kawe, M.S., Omeiza, G.K., Sani, N.A., Tenuche, O.Z., Dantong, D.D., & Tags, S.Z. (2012). Bacteriological Quality of Abattoir Effluents Discharged into Water Bodies in Abuja, Nigeria. International Scholarly Research Notices, 2012, 515689. doi:10.5402/2012/515689
Ogbomida, E.T., Kubeyinje, B., & Ezemonye, L.I. (2016). Evaluation of bacterial profile and biodegradation potential of abattoir wastewater. African Journal of Environmental Science and Technology, 10(2), 50-57. doi:10.5897/AJEST2015.1945
de Oliveira, K.M.P., Júlio, P.D.S., & Grisolia, A.B. (2013). Antimicrobial susceptibility profile of Pseudomonas spp. isolated from a swine slaughterhouse in Dourados, Mato Grosso do Sul State, Brazil. Revista Argentina de Microbiología, 45(1), 57-60.
Rabiu, A.G. & Falodun, O.I. (2017). Multi-drug Resistant Pseudomonas Species Isolated from the Wastewater of an Abattoir in Ibadan, Nigeria. Journal of Applied Life Sciences International, 13(1), 1-9. doi:10.9734/JALSI/2017/34522
Santajit, S. & Indrawattana, N. (2016). Mechanisms of Antimicrobial Resistance in ESKAPE Pathogens. BioMed Research International, 2016, 2475067. doi:10.1155/2016/2475067
Savin, M., Bierbaum, G., Hammerl, J.A. Heinemann, C., Parcina, M., Sib, E., Voigt, A., & Kreyenschmidt, J. (2020). Antibiotic-resistant bacteria and antimicrobial residues in wastewater and process water from German pig slaughterhouses and their receiving municipal wastewater treatment plants. Science of The Total Environment, 727, 138788. doi:10.1016/j.scitotenv.2020.138788
Shrivastava, S.R., Shrivastava, P.S., & Ramasamy, J. (2018). World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. Journal of Medical Society, 32(1), 76-77. doi:10.4103/jms.jms_25_17
Tesfaye, H., Alemayehu, H., Desta, A.F., & Eguale, T. (2019). Antimicrobial susceptibility profile of selected Enterobacteriaceae in wastewater samples from health facilities, abattoir, downstream rivers and a WWTP in Addis Ababa, Ethiopia. Antimicrobial Resistance and Infection Control, 8(1), 134. doi:10.1186/s13756-019-0588-1
Tran, C.S., Rangel, S.M., Almbad, H., Kierbel, A., Givskov, M., Tolker-Nielsen, T., Hauser, A.R., & Engel, J.N. (2014). The Pseudomonas aeruginosa type III translocon is required for biofilm formation at the epithelial barrier. PLoS Pathogens, 10(11), e1004479. doi:10.1371/journal.ppat.1004479
World Health Organization. (2017). WHO publishes list of bacteria for which new antibiotics are urgently needed. https://www.who.int/news-room/detail/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed
Yayan, J., Ghebremedhin, B., & Rasche, K. (2015). Antibiotic Resistance of Pseudomonas aeruginosa in Pneumonia at a Single University Hospital Center in Germany over a 10-Year Period. PLoS One, 10(10), e0139836. doi:10.1371/journal.pone.0139836
Authors
1.
Aliero AA, Jega NH, Bagudo AI, Manga SS, Hussaini K. Antibiotics Resistance Pattern of Coliform Bacteria Isolated from Slaughterhouse Wastewater in Jega Town, Kebbi State, Nigeria. Borneo J Pharm [Internet]. 2020Aug.31 [cited 2024Apr.26];3(3):170-8. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/1530
Authors continue to retain the copyright to the article if the article is published in the Borneo Journal of Pharmacy. They will also retain the publishing rights to the article without any restrictions.
Authors who publish with this journal agree to the following terms:
- Any article on the copyright is retained by the author(s).
- The author grants the journal, right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share work with an acknowledgment of the work authors and initial publications in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of published articles of work (eg, post-institutional repository) or publish it in a book, with acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to and during the submission process, as can lead to productive exchanges, as well as earlier and greater citation of published work.
- The article and any associated published material are distributed under the Creative Commons Attribution-ShareAlike 4.0 International License.
Article Details
