Standardization of Simplicia and Ethanol Extract of Purun Danau (Lepironia articulata (Retz.) Domin) Rhizome
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Submitted
6 October 2021
6 October 2021
Published
30 November 2021
30 November 2021
Abstract
Purun Danau (Lepironia articulata (Retz.) Domin) has been shown to have antimalarial and antioxidant activity. This study aimed to standardize simplicia and extract of L. articulata by determining the value of its specific and non-specific parameters. A sampling of L. articulata rhizome was carried out in Guntung Manggis, Haur Gading, and Halat. The standardization method used was based on the Indonesian Herbal Pharmacopoeia and the General Standard Parameter of Extract. The organoleptic observations showed that L. articulata simplicia was reddish-brown in color, chelated taste, and had a specific odor. Microscopic observation showed parts of this plant: epidermis, cortex, endodermis, parenchyma, bundle vessels, and scalariform vessels. Ethanol-soluble extract content was 10.00-12.66%, water-soluble extract content 8.03-10.87%, drying shrinkage 7.10-7.33%, total ash content 2.03-2.52%, acid-insoluble ash 0.33-0.42%, Pb content 5.698-9.989 ppm, Cd content 0.300-0.500 ppm, Hg content 0.070-0.090 ppm. Ethanol extract of L. articulata rhizome contained alkaloids, flavonoids, tannins, glycosides, and saponins. The yield obtained was 8.05-11.23%, total ash content was 1.58-1.67%, acid-insoluble ash was 0.23-0.33%, and water content was 7.10-8.50%. Standardization of simplicia and ethanol extract of L. articulata rhizome has met the criteria.
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References
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20. Jacquemin D, Preat J, Charlot M, Wathelet V, André JM, Perpète EA. Theoretical investigation of substituted anthraquinone dyes. J Chem Phys. 2004;121(4):1736-43. doi:10.1063/1.1764497
21. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010;2(12):1231-46. doi:10.3390/nu2121231
22. Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol. 2011;3(12):10-4.
23. Sutomo, Lestari HD, Arnida, Sriyono A. Simplicia and Extracts Standardization from Jualing Leaves (Micromelum minutum Wight & Arn.) from South Kalimantan. Borneo J Pharm. 2019;2(2):55-62. doi:10.33084/bjop.v2i2.898
24. Yang L, Wen KS, Ruan X, Zhao YX, Wei F, Wang Q. Response of Plant Secondary Metabolites to Environmental Factors. Molecules. 2018;23(4):762. doi:10.3390/molecules23040762
25. Marliana SD, Suryanti V, Suyono. The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.). Asian J Nat Prod Biochem. 2005;3(1):26-31. doi:10.13057/biofar/f030106
26. Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20. doi:10.1186/s13020-018-0177-x
27. Abubakar AR, Haque M. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. J Pharm Bioallied Sci. 2020;12(1):1-10. doi:10.4103/jpbs.JPBS_175_19
28. Badan Pengawas Obat dan Makanan Republik Indonesia. Peraturan Badan Pengawas Obat dan Makanan Nomor 32 Tahun 2019 tentang Persyaratan Keamanan dan Mutu Obat Tradisional. Jakarta, Indonesia: Badan Pengawas Obat dan Makanan Republik Indonesia; 2019.
29. Putranti W, Dewi NA, Widyastuti L. Standardization of extract and characterization of emulgel formula of lengkuas (Alpinia galanga (L.) Willd) rhizome extract. J Farm Sains Komun. 2018;15(2):81-91. doi:10.24071/jpsc.001612
30. Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KM. Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol. 2014;7(2):60-72. doi:10.2478/intox-2014-0009
31. Pavarini DP, Pavarini SP, Niehues M, Lopes NP. Exogenous influences on plant secondary metabolite levels. Anim Feed Sci Technol. 2012;176(1-4):5-16. doi:10.1016/j.anifeedsci.2012.07.002
32. Towaha J. Kandungan Senyawa Polifenol pada Biji Kakao dan Kontribusinya terhadap Kesehatan. Sirinov. 2014;2(1):1-16.
33. Kennedy DO, Wightman EL. Herbal Extracts and Phytochemicals: Plant Secondary Metabolites and the Enhancement of Human Brain Function. Adv Nutr. 2011;2(1):32-50. doi:10.3945/an.110.000117
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2. Kumar SJU, Chaitanya KMJ, Semotiuk AJ, Khrisna V. Indigenous knowledge on medicinal plants used by ethnic communities of South India. Ethnobot Res Appl. 2019;18(4):1-112. doi:10.32859/era.18.4.1-112
3. Salmerón-Manzano E, Garrido-Cardenas JA, Manzano-Agugliaro F. Worldwide Research Trends on Medicinal Plants. Int J Environ Res Public Health. 2020;17(10):3376. doi:10.3390/ijerph17103376
4. Domyos P, Te-Chato S. In vitro propagation of Lepironia articulata in Kuan Kreng peat lands, Nakhon Si Thammrat. Int J Agric Tech. 2013;9(6):1595-605.
5. Widhoyo H, Kurdiansyah, Yuniarti. Uji fitokimia pada tumbuhan purun danau (Lepironia articulata). J Sylva Scienteae. 2019;2(3):484-92. doi:10.20527/jss.v2i3.1828
6. Prychid CJ, Bruhl JJ. Floral ontogeny and gene protein localization rules out euanthial interpretation of reproductive units in Lepironia (Cyperaceae, Mapanioideae, Chrysitricheae). Ann Bot. 2013;112(1):161-77. doi:10.1093/aob/mct111
7. Sutomo, Hasanah N, Arnida, Sriyono A. Standardisasi simplisia dan ekstrak daun matoa (Pometia pinnata J.R Forst & G. Forst) asal kalimantan selatan. J Pharmascience. 2021;8(1):101-10. doi:10.20527/jps.v8i1.10275
8. Balekundri A, Mannur V. Quality control of the traditional herbs and herbal products: a review. Futur J Pharm Sci. 2020;6:67. doi:10.1186/s43094-020-00091-5
9. Arnida, Sutomo, Humairah SZA, Fadlilahturrahmah. Heme Polymerization Inhibitory Activity and Phytochemical Screening of Ethyl Acetate Fraction in Manuran (Coptosapelta tomentosa Valeton ex K. Heyne) Stem. J Jamu Indones. 2021;6(1):1-7.
10. Erickson MD, Giguere MT, Whitaker DA. Comparison of Common Solvent Evaporation Techniques in Organic Analysis. Anal Lett. 1981;14(11):841-57. doi:10.1080/00032718108081439
11. Kementerian Kesehatan Republik Indonesia. Farmakope Herbal Indonesia Edisi III. Jakarta, Indonesia: Kementerian Kesehatan Republik Indonesia; 2017.
12. Kementerian Kesehatan Republik Indonesia. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta, Indonesia: Kementerian Kesehatan Republik Indonesia; 2000.
13. Badan Standarisasi Nasional Republik Indonesia. Penentuan kadar logam berat timbal (Pb) dan cadmium (Cd) pada produk Perikanan. SNI 2354.5:2011. Jakarta, Indonesia: Badan Standarisasi Nasional Republik Indonesia; 2011.
14. Harborne JB. Phytochemical Methods: A guide to modern techniques of plant analysis. Dordrecht, Netherlands: Springer; 1984. doi:10.1007/978-94-009-5570-7
15. Vernanda RY, Puspitasari MR, Satya HN. Standarisasi Spesifik dan Non Spesifik Simplisia dan Ekstrak Etanol Bawang Putih Tunggal Terfermentasi (Allium sativum Linn.). J Farmasi Sains Terapan. 2019;6(2):74-83. doi:10.33508/jfst.v6i2.2234
16. Sutomo, Fahriah, Arnida. Skrining fitokimia dan uji aktivitas antibakteri ekstrak etanol daun racun ayam (Brucea javanica [L.] Merr.) asal kalimantan selatan. J Ilmiah Ibnu Sina. 2021;6(1):59-68. doi:10.36387/jiis.v6i1.607
17. Trase GE, Evans WC. Pharmacognosy 13th Edition. London, UK: Baillière Tindall. p. 176-80.
18. Yamin Y, Ruslin R, Sabarudin S, Sida N, Kasmawati H, Diman LOM. Determination of Antiradical Activity, Total Phenolic, and Total Flavonoid Contents of Extracts and Fractions of Langsat (Lansium domesticum Coor.) Seeds. Borneo J Pharm. 2020;3(4):249-56. doi:10.33084/bjop.v3i4.1500
19. Mughrbi HN, Auzi AA, Maghrbi H. Phytochemicals, Nutritional Value, Antioxidant, and Anticoagulant Activity of Lactuca sativa L. Leaves and Stems. Borneo J Pharm. 2020;3(3):152-61. doi:10.33084/bjop.v3i3.1394
20. Jacquemin D, Preat J, Charlot M, Wathelet V, André JM, Perpète EA. Theoretical investigation of substituted anthraquinone dyes. J Chem Phys. 2004;121(4):1736-43. doi:10.1063/1.1764497
21. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010;2(12):1231-46. doi:10.3390/nu2121231
22. Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol. 2011;3(12):10-4.
23. Sutomo, Lestari HD, Arnida, Sriyono A. Simplicia and Extracts Standardization from Jualing Leaves (Micromelum minutum Wight & Arn.) from South Kalimantan. Borneo J Pharm. 2019;2(2):55-62. doi:10.33084/bjop.v2i2.898
24. Yang L, Wen KS, Ruan X, Zhao YX, Wei F, Wang Q. Response of Plant Secondary Metabolites to Environmental Factors. Molecules. 2018;23(4):762. doi:10.3390/molecules23040762
25. Marliana SD, Suryanti V, Suyono. The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.). Asian J Nat Prod Biochem. 2005;3(1):26-31. doi:10.13057/biofar/f030106
26. Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20. doi:10.1186/s13020-018-0177-x
27. Abubakar AR, Haque M. Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. J Pharm Bioallied Sci. 2020;12(1):1-10. doi:10.4103/jpbs.JPBS_175_19
28. Badan Pengawas Obat dan Makanan Republik Indonesia. Peraturan Badan Pengawas Obat dan Makanan Nomor 32 Tahun 2019 tentang Persyaratan Keamanan dan Mutu Obat Tradisional. Jakarta, Indonesia: Badan Pengawas Obat dan Makanan Republik Indonesia; 2019.
29. Putranti W, Dewi NA, Widyastuti L. Standardization of extract and characterization of emulgel formula of lengkuas (Alpinia galanga (L.) Willd) rhizome extract. J Farm Sains Komun. 2018;15(2):81-91. doi:10.24071/jpsc.001612
30. Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KM. Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol. 2014;7(2):60-72. doi:10.2478/intox-2014-0009
31. Pavarini DP, Pavarini SP, Niehues M, Lopes NP. Exogenous influences on plant secondary metabolite levels. Anim Feed Sci Technol. 2012;176(1-4):5-16. doi:10.1016/j.anifeedsci.2012.07.002
32. Towaha J. Kandungan Senyawa Polifenol pada Biji Kakao dan Kontribusinya terhadap Kesehatan. Sirinov. 2014;2(1):1-16.
33. Kennedy DO, Wightman EL. Herbal Extracts and Phytochemicals: Plant Secondary Metabolites and the Enhancement of Human Brain Function. Adv Nutr. 2011;2(1):32-50. doi:10.3945/an.110.000117
Authors
1.
Arnida A, Maulidia M, Khairunnisa A, Sutomo S, Faisal F. Standardization of Simplicia and Ethanol Extract of Purun Danau (Lepironia articulata (Retz.) Domin) Rhizome. Borneo J Pharm [Internet]. 2021Nov.30 [cited 2025Apr.16];4(4):273-82. Available from: https://journal.umpr.ac.id/index.php/bjop/article/view/2794
Copyright (c) 2021 Arnida Arnida, Maulidia Maulidia, Amalia Khairunnisa, Sutomo Sutomo, Faisal Faisal
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