Granulation Methods Dependent Physical Characteristics of Chitosan Effervescent Granule Suspension from Mangrove Crab (Scylla serrata) Shell
DOI:
https://doi.org/10.33084/bjop.v8i4.7334Keywords:
Chitosan, Effervescent Granule, Granulation Method, Physical Characteristic, Scylla serrataAbstract
The mangrove crab (Scylla serrata), a prevalent resource in coastal mangrove ecosystems, is widely utilized for consumption. Its shell offers a valuable source of chitosan, a biopolymer readily synthesized and recognized for its potential health benefits. Chitosan's unique structure, characterized by the presence of amine groups, imparts a strong positive charge, enabling it to effectively bind with negatively charged molecules such as oils and fats. This property makes chitosan a promising natural compound for managing cholesterol levels. Developing a palatable and convenient dosage form, such as an effervescent granule suspension, is crucial to enhance its public acceptance as a health supplement. This study investigated how different granulation methods (wet and dry) influence the physical properties of effervescent granules containing chitosan, both before and after reconstitution. Four distinct formulations were prepared: F1 and F2 employed dry and wet granulation, respectively, with a citric acid : tartaric acid ratio of 10% : 20%; while F3 and F4 utilized the same granulation methods but with a higher acid ratio of 13% : 26%. Comprehensive evaluation revealed that all formulations generally met quality requirements, except F3's pH (4.38 ± 0.57). Statistical analysis using an independent sample t-test indicated that the granulation method significantly affected parameters such as flow time, percentage of fines, dispersion time, and pH. Ultimately, the dry granulation method yielded the most favorable characteristics, with Formula I (F1) exhibiting superior flow properties, optimal pH, and desired viscosity.
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Raniah N, Henri H, Kurniawan K. On the abundance and occurrence of the mangrove crabs, Scylla spp. (Crustacea: Portunidae) from Munjang mangrove, Bangka Belitung Island. J Riset Biol Aplikasinya. 2022;4(2):75-82. DOI: 10.26740/jrba.v4n2.p75-82
Pati SG, Paital B, Panda F, Jena S, Sahoo DK. Impacts of Habitat Quality on the Physiology, Ecology, and Economical Value of Mud Crab Scylla sp.: A Comprehensive Review. Water. 2023;15(11):2029. DOI: 10.3390/w15112029
Kozma M, Acharya B, Bissessur R. Chitin, Chitosan, and Nanochitin: Extraction, Synthesis, and Applications. Polymers. 2022;14(19):3989. DOI: 10.3390/polym14193989; PMCID: PMC9571330; PMID: 36235937
Mohite P, Shah SR, Singh S, Rajput T, Munde S, Ade N, et al. Chitosan and chito-oligosaccharide: a versatile biopolymer with endless grafting possibilities for multifarious applications. Front Bioeng Biotechnol. 2023;11:1190879. DOI: 10.3389/fbioe.2023.1190879; PMCID: PMC10235636; PMID: 37274159
Zhang S, Ma DW, Yang X, Chen L, Xia H, Wang SK, et al. The effect of chitosan on blood lipids in patients with hypercholesterolemia and hyperlipidemia: a systematic review and meta-analysis. Food Med Homology. 2025;2(4):9420061. DOI: 10.26599/FMH.2025.9420061
Ahn SI, Cho S, Choi NJ. Effectiveness of Chitosan as a Dietary Supplement in Lowering Cholesterol in Murine Models: A Meta-Analysis. Mar Drugs. 2021;19(1):26. DOI: 10.3390/md19010026; PMCID: PMC7827691; PMID: 33435383
Imtihani HN, Permatasari SN, Prasetya RA. In Vitro Evaluation of Cholesterol-Reducing Ability of Chitosan from Mangrove Crab (Scylla serrata) Shell Solid Dispersion using PVP K-30 as a Carrier. J Farmasi Galenika Galenika J Pharm. 2021;7(2):99–109. DOI: 10.22487/j24428744.2021.v7.i2.15597
Aranaz I, Alcántara AR, Civera MC, Arias C, Elorza B, Caballero AH, et al. Chitosan: An Overview of Its Properties and Applications. Polymers. 2021;13(19):3256. DOI: 10.3390/polym13193256; PMCID: PMC8512059; PMID: 34641071
Adi-Dako O, Kumadoh D, Egbi G, Okyem S, Addo PY, Nyarko A, et al. Strategies for formulation of effervescent granules of an herbal product for the management of typhoid fever. Heliyon. 2021;7(10):e08147. DOI: 10.1016/j.heliyon.2021.e08147; PMCID: PMC8551464; PMID: 34746457
Thapa P, Choi DH, Kim MS, Jeong SH. Effects of granulation process variables on the physical properties of dosage forms by combination of experimental design and principal component analysis. Asian J Pharm Sci. 2019;14(3):287-304. DOI: 10.1016/j.ajps.2018.08.006; PMCID: PMC7032112; PMID: 32104459
Paganelli S, Brugnera E, De Michele A, Facchin M, Beghetto V. Chitosan as a Bio-Based Ligand for the Production of Hydrogenation Catalysts. Molecules. 2024;29(9):2083. DOI: 10.3390/molecules29092083; PMCID: PMC11085195; PMID: 38731574
Rani KC, Parfati N, Muarofah D, Sacharia SN. Formulasi Granul Effervescent Herba Meniran (Phyllanthus niruri L.) dengan Variasi Suspending Agent Xanthan Gum, CMC-Na, dan Kombinasi CMC-Na-Mikrokristalin Selulosa RC- 591. J Sains Farm Klinis. 2020;7(1):39-51. DOI: 10.25077/jsfk.7.1.39-51.2020
Atchade BC, Ouedraogo S, Traore TK, Bonou-Selegbe I, Semde R. Formulation and Characterization of Effervescent Tablets from a Traditional Medicine Recipe for Dental Care. Int J Appl Pharm. 2025;17(6):388-94. DOI: 10.22159/ijap.2025v17i6.53815
Janković B, Injac R, Dreu R, Srcic S. Formulation and evaluation of immediate release tablets with different types of paracetamol powders prepared by direct Compression. Afr J Pharm Pharmacol. 2011;5(1):31-41. DOI: 10.5897/AJPP10.274
Guo Z, Chen X, Liu H, Guo Q, Guo X, Lu H. Theoretical and experimental investigation on angle of repose of biomass–coal blends. Fuel. 2014;116:131-9. DOI: 10.1016/j.fuel.2013.07.098
Imtihani HN, Alfreeda S, Arif JRA. Pengaruh Variasi Disintegran Avicel PH-102 dan Primogel terhadap Karakteristik Co-Processed Excipient. J Ilmiah Medicamento. 2023;9(1):9–15. DOI: 10.36733/medicamento.v9i1.4635
Laurent O, Triyanti T, Suranda D, Chiuman L. Formulation and Evaluation of Effervescent Granules Ethanol Extract of Andaliman Fruit (Zanthoxylum acanthopodium DC) with Combination of Citric Acid-Tartaric Acid and Sodium Bicarbonate. Eureka Herba Indones. 2023;4(4):310-5. DOI: 10.37275/ehi.v4i4.89
Seyrekoğlu F, Efdal E. Chitosan-Based Edible Films as Innovative Preservation Tools for Fermented and Dairy Products. Fermentation. 2025;11(9):542. DOI: 10.3390/fermentation11090542
Sun J, Wang T, Liu L, Li Q, Liu H, Wang X, et al. Preparation and Application of Edible Chitosan Coating Incorporating Natamycin. Polymers. 2025;17(8):1062. DOI: 10.3390/polym17081062; PMCID: PMC12030211; PMID: 40284327
Baral KP. Formulation and Evaluation of Quality Parameters of Effervescent Granules from the Potent Antioxidant between Two Variants of the Adaptogenic Herb Ocimum tenuiflorum L. ScientificWorldJournal. 2023;2023:2050846. DOI: 10.1155/2023/2050846; PMCID: PMC10154098; PMID: 37144025
Fayed MH, Abdel-Rahman SI, Alanazi FK, Ahmed MO, Tawfeek HM, Al-Shedfat RI. High-Shear Granulation Process: Influence of Processing Parameters on Critical Quality Attributes of Acetaminophen Granules and Tablets Using Design of Experiment Approach. Acta Pharm Pol. 2017;74(1):235-48. PMID: 29474779
Maysarah H, Sari I, Faradilla M, Kwok K. Formulation of Effervescent Granule from Robusta Green Coffee Bean Ethanolic Extract (Coffea canephora). J Pharm Bioallied Sci. 2020;12(Suppl 2):S743-6. DOI: 10.4103/jpbs.jpbs_258_19; PMCID: PMC8021061; PMID: 33828371
Abdullah HS, Imtihani HN. Formulasi dan Evaluasi Granul Dispersi Padat Ekstrak Kitosan Cangkang Kepiting Bakau (Scylla serrata) dengan Perbandingan Kitosan:PVP K-30 1:2. J Akfarindo. 2022;7(1):45–51. DOI: 10.37089/jofar.vi0.119
Shanmugam S. Granulation techniques and technologies: recent progresses. Bioimpacts. 2015;5(1):55-63. DOI: 10.15171/bi.2015.04; PMCID: PMC4401168; PMID: 25901297
Kotamarthy L, Metta N, Ramachandran R. Understanding the Effect of Granulation and Milling Process Parameters on the Quality Attributes of Milled Granules. Processes. 2020;8(6):683. DOI: 10.3390/pr8060683
Shah DS, Moravkar KK, Jha DK, Lonkar V, Amin PD, Chalikwar SS. A concise summary of powder processing methodologies for flow enhancement. Heliyon. 2023;9(6):e16498. DOI: 10.1016/j.heliyon.2023.e16498; PMCID: PMC10245010; PMID: 37292344
Jones-Salkey O, Chu Z, Ingram A, Windows-Yule CRK. Reviewing the Impact of Powder Cohesion on Continuous Direct Compression (CDC) Performance. Pharmaceutics. 2023;15(6):1587. DOI: 10.3390/pharmaceutics15061587; PMCID: PMC10305442; PMID: 37376036
Adepu S, Ramakrishna S. Controlled Drug Delivery Systems: Current Status and Future Directions. Molecules. 2021;26(19):5905. DOI: 10.3390/molecules26195905; PMCID: PMC8512302; PMID: 34641447
Huynh DTM, Hai HT, Hau NM, Lan HK, Vinh TP, Tran VD, et al. Preparations and characterizations of effervescent granules containing azithromycin solid dispersion for children and elder: Solubility enhancement, taste-masking, and digestive acidic protection. Heliyon. 2023;9(6):e16592. DOI: 10.1016/j.heliyon.2023.e16592; PMCID: PMC10245243; PMID: 37292293
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